Evaluation of metastatic potential of circulating tumor cells using quantitative phase imaging (QPI)

Василенко, И. А.; Метелин, В. Б.; Kardasheva, Ziver; Балканов, А. С.; Лифенко, Р. А.

doi:10.1117/12.2510383

Cited by 1 publication

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References 17 publications

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“…Similar approaches have been published. Vasilenko et al used laser-based QPI to address the morphology of circulating tumor cells in relationship to their metastatic potential 24 . We were more interested in drug screening and Murray et al 25 and Mir et al 26 successfully used QPI exactly for that purpose but only evaluated cell growth.…”

Section: Discussionmentioning

confidence: 99%

Addressing cancer invasion and cell motility with quantitative light microscopy

Zicha

2022

Sci Rep

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The incidence of death caused by cancer has been increasing worldwide. The growth of cancer cells is not the main problem. The majority of deaths are due to invasion and metastasis, where cancer cells actively spread from primary tumors. Our inbred rat model of spontaneous metastasis revealed dynamic phenotype changes in vitro correlating with the metastatic potential in vivo and led to a discovery of a metastasis suppressor, protein 4.1B, which affects their 2D motility on flat substrates. Subsequently, others confirmed 4.1B as metastasis suppressor using knock-out mice and patient data suggesting mechanism involving apoptosis. There is evidence that 2D motility may be differentially controlled to the 3D situation. Here we show that 4.1B affects cell motility in an invasion assay similarly to the 2D system, further supporting our original hypothesis that the role of 4.1B as metastasis suppressor is primarily mediated by its effect on motility. This is encouraging for the validity of the 2D analysis, and we propose Quantitative Phase Imaging with incoherent light source for rapid and accurate testing of cancer cell motility and growth to be of interest for personalized cancer treatment as illustrated in experiments measuring responses of human adenocarcinoma cells to selected chemotherapeutic drugs.

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Section: Discussionmentioning

confidence: 99%

Addressing cancer invasion and cell motility with quantitative light microscopy

Zicha

2022

Sci Rep

View full text Add to dashboard Cite

show abstract

Evaluation of metastatic potential of circulating tumor cells using quantitative phase imaging (QPI)

Cited by 1 publication

References 17 publications

Addressing cancer invasion and cell motility with quantitative light microscopy

Addressing cancer invasion and cell motility with quantitative light microscopy

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