Evaluation of metallo-beta-lactamase production in multiple antibiotic-resistant Pseudomonas spp. and Acinetobacter baumannii strains

Abstract

This study aimed to evaluate the metallo-beta-lactamase (MBL) production in Pseudomonas spp. and Acinetobacter baumannii using phenotypic and genotypic methods and to determine the most appropriate phenotypic method. The study included 55 Pseudomonas spp. (53 Pseudomonas aeruginosa, 1 P. fluorescens and 1 P. putida) and 33 A. baumannii isolates which were resistant to imipenem (IMP) and/or meropenem (MEM). Six phenotypic and one genotypic (real-time polymerase chain reaction [RT-PCR]) methods were used. According to the phenotypic tests, the rates of MBL-positive Pseudomonas spp. and A. baumannii were, respectively: 25.5% and 39.4% by the gradient test; 21.8% and 21.2% by the Rosco rapid CARB screen test; 9.1% and 21.2% by the modified Hodge test (MHT); 32.7% and 66.7% by the combined EDTA disk diffusion test; 56.4% and 100% by IMP þ EDTA and 49.0% and 72.7% by MEM þ EDTA and 9.1% and 3.0% by IMP þ dipicolinic acid (DPA) for the Rosco MBL confirm test; 36.4% and 6.1% by IMP þ DPA and 54.5% and 6.1% MEM þ DPA for the double disk synergy test. MBL genes were detected only in three Pseudomonas spp. (bla IMP in two P. aeruginosa isolates and bla VIM in a P. fluorescens isolate). For Pseudomonas spp., the MBL positivity rate did not significantly differ between the RT-PCR and MHT and between the RT-PCR and Rosco MBL confirm test (with IMP þ DPA) (p > 0.10). In conclusion, the Rosco MBL confirm test (with IMP þ DPA) phenotypically predicted the MBL positivity most closely to the RT-PCR method for both Pseudomonas spp. and A. baumannii isolates. ARTICLE HISTORY

Biofilm and Pathogenesis-Related Proteins in the Foodborne P. fluorescens ITEM 17298 With Distinctive Phenotypes During Cold Storage

“…Only few species are classified opportunistic human pathogens and phytopathogens ( Pseudomonas aeruginosa, Pseudomonas syringae , and Pseudomonas cichorii ; de Bentzmann and Plésiat, 2011 ; Ichinose et al, 2013 ). However, an increasing number of Pseudomonas strains, without the issue of pathogenicity, have displayed multiple drug resistances, raised from horizontal transfer of genetic elements or inheritable mutations, which pose a severe threat to human health ( Guzel et al, 2018 ; Quintieri et al, 2019a ). Moreover, the isolation of non-pathogenic pseudomonads strains from clinical human cases has rapidly increased.…”

Nozokomi̇yal Acinetobacter Baumannii İzolatlarinda Metallo-Beta-Laktamaz Akti̇vi̇tesi̇ni̇n Çeşi̇tli̇ Fenoti̇pi̇k Yöntemlerle Araştirilmasi

“…Avrupa Antimikrobiyal Direnç Sürveyans Ağı (EARS-Net) Acinetobacter türleri için karbapenem direncini 2014 yılı raporunda %0-93, 2016 yılında ise %0-95.4 olarak bildirmiştir. Orta Asya ve Doğu Avrupa Antimikrobiyal Direnç Sürveyans Ağı (CAESAR), invaziv örneklerden elde edilen A. baumannii izolatlarının 2014 raporunda %11-93'ünün 2016 raporunda %11-95'inin karbapenem dirençli olduğunu bildirmiştir (10) . 2020 CAESAR raporunda Acinetobacter türleri için karbapenem direnci 38 ülkenin üçünde %1'in altında iken, çoğunlukla Güney ve Doğu Avrupa olmak üzere 21 ülkede %50'nin üzerinde çıkmıştır (23) .…”

“…Bu mekanizmalardan birisi bakteriler tarafından karbapenemaz üretimidir. Karbapenemazlar arasında ise, metallo-beta-laktamazlar (MBL) giderek daha önemli hale gelmektedir (2,10) . İlk plazmid aracılı MBL 1991'de Pseudomonas aeruginosa'da rapor edilmiştir (6) .…”

Retrospective study of incidence of metallo-β -lactamase-producing Non-fermenters in nosocomial infections of a tertiary care hospital, in Navi Mumbai

“…The Imipenem (IMP)-EDTA combined disc test was used. 5 The Imipenem (IMP)-EDTA combined disk test was performed as described by Pseudomonas aeruginosa ATCC 27853 used as the control. Test organisms were inoculated on to plates of Mueller-Hinton agar as recommended by the CLSI.…”

“…Pseudomonas aeruginosa and Acinetobacter baumannii are among the most important causes of healthcare-associated infections. 5 As a result of being difficult to detect, such organisms pose significant risks, particularly due to their role in unnoticed spread within institutions and their ability to participate in horizontal MBL gene transfer with other pathogens in the hospital. In recent years, MBL genes have spread from P. aeruginosa, Acinetobacter Spp.…”