Evaluation of mechanical and histological properties of cryopreserved human premolars under short-term preservation: A preliminary study

Lin, Shu Li; Lee, Sheng Yang; Lin, Yen Chuang; Huang, Yen Hua; Yang, Jen Chang; Huang, Haw Ming

doi:10.1016/j.jds.2013.04.010

Cited by 8 publications

(3 citation statements)

References 19 publications

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“…If degradation did occur, the effect of freezing on the mechanical properties was smaller than the natural variation of those properties across a sample before freezing (24). A similar study of dental pulp showed that storage in transportation solution for 24 h had no significant negative effects on the histological or mechanical properties of the pulp tissue extracted from the cryopreserved intact teeth (25). When evaluating the effects of freezing on the periodontal ligament, force/displacement curves generated to evaluate the behavior of the periodontal ligament in autopsy specimens were comparable with those previously derived by in vivo measurements (26).…”

Section: Discussionmentioning

confidence: 99%

Fracture Limits of Maxillary Fourth Premolar Teeth in Domestic Dogs Under Applied Forces

et al. 2019

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A cadaveric study was performed to investigate the external mechanical forces required to fracture maxillary fourth premolar teeth in domestic dogs and describe a clinically relevant model of chewing forces placed on functionally important teeth in which fracture patterns are consistent with those defined by the American Veterinary Dental College (AVDC). Twenty-four maxillary fourth premolar teeth were harvested from dog cadavers. Samples consisted of teeth with surrounding alveolar bone potted in polycarbonate cylinders filled with acrylic. The cylinders were held by an aluminum device at an angle of 60° with respect to the ground. An axial compression test was performed, creating a force upon the occluso-palatal aspects of the main cusps of the crowns of the teeth. The highest compressive force prior to failure was considered the maximum force sustained by the teeth. Results showed the mean maximum force (± SD) sustained by the tested teeth at the point of fracture was 1,281 N (± 403 N) at a mean impact angle (± SD) of 59.7° (± 5.2°). The most common fracture type that occurred among all samples was a complicated crown fracture (n = 12), followed by an uncomplicated crown fracture (n = 6), complicated crown-root fracture (n = 5), and uncomplicated crown-root fracture (n = 1). There was no statistically significant correlation between dog breed, age, weight, impact angle, crown height or crown diameter, and the maximum force applied at the point of fracture. The only independent variable that remained significantly associated with maximum force was the crown height to diameter ratio (p = 0.005), suggesting that a decreased ratio increases tooth fracture resistance. The methodology described herein has been successful in creating a pattern of fracture of maxillary fourth premolar teeth consistent with that defined by the AVDC under angled compression at forces within the maximum chewing capability of the average domestic dog.

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Section: Discussionmentioning

confidence: 99%

Fracture Limits of Maxillary Fourth Premolar Teeth in Domestic Dogs Under Applied Forces

et al. 2019

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“…In addition, tooth banking for tooth autotransplantation has also been successfully performed using this method, showing satisfactory implantation outcomes in patients [111,112]. At histological level, magnetic cryopreservation retained tissue architecture of tooth under storage, maintaining viable cells of odontoblastic region and cell-rich zone, where MSCs from dental pulp reside, while traditional cryopreservation disrupted and damaged the tissue [113,114].…”

Section: Cryopreservation and Bankingmentioning

confidence: 99%

Dental Pulp Stem Cells: Current Advances in Isolation, Expansion and Preservation

Rodas-Junco

Villicaña

2017

Tissue Eng Regen Med

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Dental pulp stem cells (DPSCs) are mesenchymal stem cells with high self-renewal potential that have the ability to differentiate into several cell types. Thus, DPSCs have become a promising source of cells for several applications in regenerative medicine, tissue engineering, and stem cell therapy. Numerous methods have been reported for the isolation, expansion, and preservation of DPSCs. However, methods are diverse and do not follow specific rules or parameters, which can affect stem cell properties, adding more variation to experimental results. In this review, we compare and analyze current experimental evidence to propose some factors that can be useful to establish better methods or improved protocols to prolong the quality of DPSCs. In addition, we highlight other factors related to biological aspects of dental tissue source (e.g., age, genetic background) that should be considered before tooth selection. Although current methods have reached significant advances, optimization is still required to improve culture stability and its maintenance for an extended period without losing stem cell properties. In addition, there is still much that needs to be done toward clinical application due to the fact that most of DPSCs procedures are not currently following good manufacturing practices. The establishment of optimized general or tailored protocols will allow obtaining well-defined DPSCs cultures with specific properties, which enable more reproducible results that will be the basis to develop effective and safe therapies.

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“…Such structures of spongy nature have been proven to allow and promote bone differentiation [ 38 ]. Although the produced scaffolds provided an excellent biocompatible 3D environment for in vitro biological evaluation, their elastic modulus values were mechanically inferior compared to the native dental pulp tissue [ 39 ], which may limit their use as substitutes. Regarding degradable materials, when cells are seeded onto scaffolds, two antagonistic procedures constantly take place: the degradation of the scaffold, and the de novo synthesis of the extracellular matrix by the cells.…”

Section: Discussionmentioning

confidence: 99%

Kappa-Carrageenan/Chitosan/Gelatin Scaffolds Provide a Biomimetic Microenvironment for Dentin-Pulp Regeneration

Loukelis

Machla

Bakopoulou

et al. 2023

IJMS

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This study aims to investigate the impact of kappa-carrageenan on dental pulp stem cells (DPSCs) behavior in terms of biocompatibility and odontogenic differentiation potential when it is utilized as a component for the production of 3D sponge-like scaffolds. For this purpose, we prepared three types of scaffolds by freeze-drying (i) kappa-carrageenan/chitosan/gelatin enriched with KCl (KCG-KCl) as a physical crosslinker for the sulfate groups of kappa-carrageenan, (ii) kappa-carrageenan/chitosan/gelatin (KCG) and (iii) chitosan/gelatin (CG) scaffolds as a control. The mechanical analysis illustrated a significantly higher elastic modulus of the cell-laden scaffolds compared to the cell-free ones after 14 and 28 days with values ranging from 25 to 40 kPa, showing an increase of 27–36%, with the KCG-KCl scaffolds indicating the highest and CG the lowest values. Cell viability data showed a significant increase from days 3 to 7 and up to day 14 for all scaffold compositions. Significantly increasing alkaline phosphatase (ALP) activity has been observed over time in all three scaffold compositions, while the KCG-KCl scaffolds indicated significantly higher calcium production after 21 and 28 days compared to the CG control. The gene expression analysis of the odontogenic markers DSPP, ALP and RunX2 revealed a two-fold higher upregulation of DSPP in KCG-KCl scaffolds at day 14 compared to the other two compositions. A significant increase of the RunX2 expression between days 7 and 14 was observed for all scaffolds, with a significantly higher increase of at least twelve-fold for the kappa-carrageenan containing scaffolds, which exhibited an earlier ALP gene expression compared to the CG. Our results demonstrate that the integration of kappa-carrageenan in scaffolds significantly enhanced the odontogenic potential of DPSCs and supports dentin-pulp regeneration.

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Evaluation of mechanical and histological properties of cryopreserved human premolars under short-term preservation: A preliminary study

Cited by 8 publications

References 19 publications

Fracture Limits of Maxillary Fourth Premolar Teeth in Domestic Dogs Under Applied Forces

Fracture Limits of Maxillary Fourth Premolar Teeth in Domestic Dogs Under Applied Forces

Dental Pulp Stem Cells: Current Advances in Isolation, Expansion and Preservation

Kappa-Carrageenan/Chitosan/Gelatin Scaffolds Provide a Biomimetic Microenvironment for Dentin-Pulp Regeneration

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