Evaluation of Mechanical Allodynia in an Animal Immobilization Model Using the Von Frey Method

Trierweiler, Jaqueline; Göttert, Débora Negrini; Gehlen, Günther

doi:10.1016/j.jmpt.2011.09.007

Cited by 21 publications

(7 citation statements)

References 51 publications

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“…These problems may be related to both the back pain and gastrointestinal problems reported by a majority of astronauts and bed-ridden human patients (Styf et al 2001). Furthermore, understanding limb immobilization-induced mechanical hyperalgesia in humans and animals (Hirose et al 2001;Terkelsen et al 2008;Ohmichi et al 2012;Trierweiler et al 2012), and pathogenesis of bone fracture and neuropathy risks in diabetes (Dobretsov et al 2007; Leslie et al 2007) provide additional rationale for this study.…”

Section: Introductionmentioning

confidence: 97%

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Animal model of simulated microgravity: a comparative study of hindlimb unloading via tail versus pelvic suspension

et al. 2013

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The aim of this study was to compare physiological effects of hindlimb suspension (HLS) in tail- and pelvic-HLS rat models to determine if severe stretch in the tail-HLS rats lumbosacral skeleton may contribute to the changes traditionally attributed to simulated microgravity and musculoskeletal disuse in the tail-HLS model. Adult male Sprague-Dawley rats divided into suspended and control-nonsuspended groups were subjected to two separate methods of suspension and maintained with regular food and water for 2 weeks. Body weights, food and water consumption, soleus muscle weight, tibial bone mineral density, random plasma insulin, and hindlimb pain on pressure threshold (PPT) were measured. X-ray analysis demonstrated severe lordosis in tail- but not pelvic-HLS animals. However, growth retardation, food consumption, and soleus muscle weight and tibial bone density (decreased relative to control) did not differ between two HLS models. Furthermore, HLS rats developed similar levels of insulinopenia and mechanical hyperalgesia (decreased PPT) in both tail- and pelvic-HLS groups. In the rat-to-rat comparisons, the growth retardation and the decreased PPT observed in HLS-rats was most associated with insulinopenia. In conclusion, these data suggest that HLS results in mild prediabetic state with some signs of pressure hyperalgesia, but lumbosacral skeleton stretch plays little role, if any, in these pathological changes.

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Section: Introductionmentioning

confidence: 97%

“…; Trierweiler et al. ), and pathogenesis of bone fracture and neuropathy risks in diabetes (Dobretsov et al. ; Leslie et al.…”

Section: Introductionmentioning

confidence: 99%

Animal model of simulated microgravity: a comparative study of hindlimb unloading via tail versus pelvic suspension

et al. 2013

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“…The remaining three rabbits were used as healthy controls. The surgery was performed under general anesthesia (intramuscular administration of 20 mg/ml xylazine (Rompun, Bayer, Kiel, Germany) and 50 mg/ml ketamine (Ketolar, Pfizer, Hameln, Germany) at 3:1 ratio), under aseptic conditions in the operating room 22 – 24 .After 2 weeks of surgery, CM was performed using ActivatorV at setting 2 with preload of 3.705 lb/inch spring rate applied to the tibial tubercle of the rabbit right hind limb (true manipulation, TM) at a 90° angle from medial to lateral side 20 , 25 . The contralateral left hind limb received a false manipulation (FM) consisting of ActivatorV firing in the air and gently touching the tibial tubercle.…”

Section: Methodsmentioning

confidence: 99%

Beneficial effects of manually assisted chiropractic adjusting instrument in a rabbit model of osteoarthritis

Conesa-Buendía

Mediero

Fujikawa

et al. 2020

Sci Rep

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Osteoarthritis (OA) is a degenerative disease characterized by injury of all joint tissues. Our previous study showed that in experimental osteoporosis, chiropractic manipulation (CM) exerts protective effects on bone. We here assessed whether CM might ameliorate OA by improving subchondral bone sclerosis, cartilage integrity and synovitis. Male New-Zealand rabbits underwent knee surgery to induce OA by anterior cruciate ligament injury. CM was performed using the chiropractic instrument ActivatorV 3 times/week for 8 weeks as follows: force 2 setting was applied to the tibial tubercle of the rabbit right hind limb (TM-OA), whereas the corresponding left hind limb received a false manipulation (FM-OA) consisting of ActivatorV firing in the air and slightly touching the tibial tubercle. After sacrifice, subchondral bone integrity was assessed in the tibiae by microCT and histology. Cartilage damage and synovitis were estimated by Mankin's and Krenn's scores, respectively, and histological techniques. Bone mineral density and content in both cortical and trabecular compartments of subchondral bone decreased in OA rabbits compared to controls, but partially reversed in the TM-OA group. Trabecular bone parameters in the latter group also showed a significant improvement compared to FM-OA group. Moreover RANKL, OPG, ALP and TRAP protein expression in subchondral bone significantly decreased in TM-OA rabbits with respect to FM-OA group. CM was associated with lower Mankin's and Krenn's scores and macrophage infiltrate together with a decreased protein expression of pro-inflammatory, fibrotic and angiogenic factors, in TM-OA rabbits with respect to FM-OA. Our results suggest that CM may mitigate OA progression by improving subchondral bone as well as cartilage and synovial membrane status. Osteoarthritis (OA) is one of the most common chronic diseases affecting all anatomical structures of the joint, namely cartilage, subchondral bone and synovial membrane 1. This disease affects about 15% of the population aged 25-75 years, and its prevalence significantly increases with age, affecting 70% of the population over 65 years 2. Although OA has been described as a cartilage disorder, changes in the underlying (subchondral) bone also occur in this disease 3. In this sense, different molecular alterations associated with the latter bone remodeling, e.g., in expression of nuclear factor ligand receptor kappa B (RANKL) and osteoprotegerin (OPG), have been described in OA 4-7. Preclinical and clinical studies point to the observed alterations in subchondral bone as an important OA pathogenic factor 8. In fact, studies in animal models of combined osteoporosis (OP) and OA (OPOA) demonstrate that OP induces cartilage damage 9. In this setting, the observed significant correlation between deterioration of subchondral bone and cartilage injury indicates that alterations in subchondral microstructure aggravate cartilage damage 10 .

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“…Von Frey tests were performed as previously described 44,45 . Briefly, rats were placed in small individual cages with mesh wire at the bottom.…”

Section: Assessment Of Functional Regenerationmentioning

confidence: 99%

“…For behavioral assessments, BBB tests were performed twice pre-injury and again at days 1, 4, 7, and then once weekly post-injury. Von Frey tests were performed once pre-injury and at days12,24,35,45, 56, 88, and 112 post-injury. At endpoint, animals were necropsied, and hearts were harvested for histological analyses.…”

mentioning

confidence: 99%

A Long-Term Pilot Study on Sex and Spinal Cord Injury Shows Sexual Dimorphism in Functional Recovery and Cardio-Metabolic Responses

Ghnenis¹,

Burns²,

Osimanjiang³

et al. 2020

Sci Rep

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More than a quarter of a million individuals in the US live with spinal cord injury (Sci). Sci disrupts neural circuitry to vital organs in the body. Despite severe incidences of long-term peripheral complications from Sci, the cardio-metabolic consequences and divergences in sex-related responses are not well described. We examined the effects of SCI on functional recovery, cardiac structure and function, body composition, and glucose metabolism on adult female and male Sprague Dawley (SD) rats. Sci was induced at T10 via contusion. Measured outcomes include behavioral assessment, body weight, dualenergy X-ray absorptiometry (DeXA) for body composition, echocardiography for cardiac structure and function, intraperitoneal glucose tolerance test (ipGtt) for glucose metabolism, insulin tolerance test (itt), and histology of cardiac structure at the endpoint. there was a decrease in body fat percentage in both sexes, with SCI females disproportionately affected in percent body fat change. Left ventricular internal diameter during systole (LViDs) was decreased in Sci females more than in Sci males. no significant differences in glucose metabolism were observed up to 20 weeks post-injury (PI). These data show significant cardio-metabolic differences as a consequence of SCI and, furthermore, that sex is an underlying factor in these differences.

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Evaluation of Mechanical Allodynia in an Animal Immobilization Model Using the Von Frey Method

Cited by 21 publications

References 51 publications

Animal model of simulated microgravity: a comparative study of hindlimb unloading via tail versus pelvic suspension

Animal model of simulated microgravity: a comparative study of hindlimb unloading via tail versus pelvic suspension

Beneficial effects of manually assisted chiropractic adjusting instrument in a rabbit model of osteoarthritis

A Long-Term Pilot Study on Sex and Spinal Cord Injury Shows Sexual Dimorphism in Functional Recovery and Cardio-Metabolic Responses

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