Evaluation of Maternal Reproductive Outcomes and Biochemical Analysis from Wistar Audiogenic Rats (WAR) and Repercussions in Their Offspring

Moraes-Souza, Rafaianne Queiroz; Sinzato, Yuri Karen; Antunes, Beatriz T; Umeoka, Eduardo Henrique de Lima; Oliveira, José Antônio Cortes de; García-Cairasco, Norberto; Karki, Barshana; Volpato, Gustavo Tadeu; Damasceno, Débora Cristina

doi:10.1007/s43032-020-00236-0

“…Previous data from our laboratory confirm these findings [35]. Although WAR animals do not differ in number of born pups as compared to Wistar [35], a study from our group revealed that the WAR strain presented hyperproteinemia, hypertriglyceridemia, and embryonic losses suggesting an inadequate intrauterine condition for embryonic development and fetal viability [36]. WARs present both reduced weight at birth and reduced body weight gain during the lactation period and adulthood [28,35] and a recent study revealed a higher percentage of skeletal anomalies in fetuses compared with Wistar [36].…”

Section: Discussionsupporting

confidence: 83%

Maternal behavior and the neonatal HPA axis in the Wistar Audiogenic Rat (WAR) strain: Early-life implications for a genetic animal model in epilepsy

Godoy

¹

,

García-Cairasco

²

2021

Epilepsy & Behavior

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Epileptogenesis is a multistage process and seizure susceptibility can be influenced by stress early in life. Wistar Audiogenic Rat (WAR) strain is an interesting model to study the association between stress and epilepsy, since it is naturally susceptible to seizures and present changes in the hypothalamus-pituitary-adrenal (HPA) axis activity. All these features are related to the pathogenic mechanisms usually associated to psychiatric comorbidities present in epilepsy. Therefore, the current study aimed to evaluate the neonate HPA axis function and maternal care under control and stress conditions in the WAR strain. Maternal behavior and neonate HPA axis were evaluated in Wistar and WAR strains under rest and after the presence of stressors. We observed that WAR pups present higher plasmatic corticosterone concentration as compared to Wistar pups. Although WAR dams do not show significant altered maternal behavior at rest, there is a higher latency to recover the litter in the pup retrieval test, while some did not recover all the litter. WAR dams presented similar behaviors to Wistar dams to a female intruder and maternal care with the pups in the maternal defense test. Taken together, these findings indicate that the WAR strain could show HPA axis disruption early in life and dams present altered maternal behavior under stressful events. Those alterations make the WAR strain an interesting model to evaluate vulnerability to epilepsy and its associated neuropsychiatric comorbidities.

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“…The placental efficiency was calculated by using the ratio between the fetal and the placental weight. 24…”

Section: Pregnancy Protocolsmentioning

confidence: 99%

Comparison of streptozotocin-induced diabetes at different moments of the life of female rats for translational studies

Sinzato

¹

,

Klöppel

²

,

Miranda

³

et al. 2021

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Animal models are widely used for studying diabetes in translational research. However, methods for induction of diabetes are conflicting with regards to their efficacy, reproducibility and cost. A comparison of outcomes between the diabetic models is still unknown, especially full-term pregnancy.To understand the comparison, we analyzed the streptozotocin (STZ)-induced diabetes at three life-different moments during the neonatal period in Sprague–Dawley female rats: at the first (D1), second (D2) and fifth (D5) day of postnatal life. At adulthood (90 days; D90), the animals were submitted to an oral glucose tolerance test (OGTT) for diabetic status confirmation. The diabetic and control rats were mated and sacrificed at full-term pregnancy for different analyses. Group D1 presented a higher mortality percentage after STZ administration than groups D2 and D5. All diabetic groups presented higher blood glucose levels as compared to those of the control group, while group D5 had higher levels of glycemia compared with other groups during OGTT. The diabetic groups showed impaired reproductive outcomes compared with the control group. Group D1 had lower percentages of mated rats and D5 showed a lower percentage of a full-term pregnancy. Besides that, these two groups also showed the highest percentages of inadequate fetal weight. In summary, although all groups fulfill the diagnosis criteria for diabetes in adult life, in our investigation diabetes induced on D5 presents lower costs and higher efficacy and reproducibility for studies involving diabetes-complicated pregnancy.

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“…Although WAR animals do not differ in number of born pups as compared to Wistar [35], a study from our group revealed that the WAR strain presented hyperproteinemia, hypertriglyceridemia, and embryonic losses suggesting an inadequate intrauterine condition for embryonic development and fetal viability [36]. WARs present both reduced weight at birth and reduced body weight gain during the lactation period and adulthood [28,35] and a recent study revealed a higher percentage of skeletal anomalies in fetuses compared with Wistar [36].…”

Section: Discussionmentioning

confidence: 99%

Maternal behavior and the neonatal HPA axis in the Wistar Audiogenic Rat (WAR) strain: early life implications for a genetic animal model in epilepsy

Godoy

¹

,

García-Cairasco

²

2021

Preprint

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1

0

View full text Add to dashboard Cite

Epileptogenesis is a multistage process and seizure susceptibility can be influenced by stress early in life. Wistar Audiogenic Rat (WAR) strain is an interesting model to study the association between stress and epilepsy, since it is naturally susceptible to seizures and present changes in the hypothalamus-pituitary-adrenal (HPA) axis activity. All these features are related to the pathogenic mechanisms usually associated to psychiatric comorbidities present in epilepsy. Therefore, the current study aimed to evaluate the neonate HPA axis function and maternal care under control and stress conditions in the WAR strain. Maternal behavior and neonate HPA axis were evaluated in Wistar and WAR strains under rest and after the presence of stressors. We observed that WAR pups present higher plasmatic corticosterone concentration as compared to Wistar pups. Although WAR dams do not show significant altered maternal behavior at rest, there is a higher latency to recover the litter in the pup retrieval test, while some did not recover all the litter. WAR dams presented similar behaviors to Wistar dams to a female intruder and maternal care with the pups in the maternal defense test. Taken together, these findings indicate that the WAR strain could show HPA axis disruption early in life and dams present altered maternal behavior under stressful events. Those alterations make the WAR strain an interesting model to evaluate vulnerability to epilepsy and its associated neuropsychiatric comorbidities.

show abstract

Evaluation of Maternal Reproductive Outcomes and Biochemical Analysis from Wistar Audiogenic Rats (WAR) and Repercussions in Their Offspring

Cited by 6 publications

References 47 publications

Maternal behavior and the neonatal HPA axis in the Wistar Audiogenic Rat (WAR) strain: Early-life implications for a genetic animal model in epilepsy

Maternal behavior and the neonatal HPA axis in the Wistar Audiogenic Rat (WAR) strain: Early-life implications for a genetic animal model in epilepsy

Comparison of streptozotocin-induced diabetes at different moments of the life of female rats for translational studies

Maternal behavior and the neonatal HPA axis in the Wistar Audiogenic Rat (WAR) strain: early life implications for a genetic animal model in epilepsy

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