Evaluation of MASTDISCS
            <i>combi Carba plus</i>
            for the identification of metallo‐β‐lactamases, KPC and OXA‐48 carbapenemase genes in
            <i>Enterobacteriaceae</i>
            clinical isolates

Li, M.; Lü, Wei; Guo, Shuliang; Li, J.

doi:10.1111/lam.13240

Cited by 17 publications

(23 citation statements)

References 17 publications

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“…In the current study, the following antimicrobials were tested: piperacillin and piperacillin-tazobactam (β-lactams and β-lactamase-inhibitor combination); ceftazidime, ceftriaxone, cefazoline, cefepime and cefoxitin (cephalosporins); gentamicin and amikacin (aminoglycosides); imipenem, ertapenem and meropenem (carbapenems); ciprofloxacin (quinolone); trimethoprim/sulphamethoxazole (sulphonamide); tigecycline (tetracycline); and nitrofurantoin (in urinary infection). They were determined for all these isolates using the Vitek-2 automated (GN-20 and 22 AST card) system (bioMerieux, USA) according to the manufacturer’s instructions [ 10 ]. The resistance profiles were categorised into MDR, XDR and PDR according to Algammal [ 1 ].…”

Section: Methodsmentioning

confidence: 99%

“…Carbapenemase production was examined phenotypically for all strains that showed resistance or intermediate resistance to carbapenem drugs, or demonstrated resistance to either third-generation cephalosporins or cefoxitin, using the MASTDISCS® Combi Carba plus (Carba plus) commercial combination disc assay, according to the manufacturer’s instructions [ 10 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…AmpC production with porin loss is indicated when both discs C and D show a zone difference of ≥5 mm compared to disc A. In the absence of such synergistic effects among discs A–D, the diameter of the inhibition zone of ≤10 mm around disc E indicated OXA-48 carbapenemase production [ 10 ]. K .…”

Section: Methodsmentioning

confidence: 99%

“…These Gram-negative bacteria are the main nosocomial pathogens and the most prevalent bacteria responsible for a range of hospital-acquired and community-acquired difficult to treat infections such as meningitis, pneumonia, peritonitis, septicaemia and urinary tract infections. Infection acquired by carbapenemase-producing Gram-negative bacilli is associated with high in-hospital mortality (up to 70%) due to the inefficiency of initial treatment regimens [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and characterisation of carbapenemase encoding genes in multidrug-resistant Gram-negative bacilli

2021

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Background Emerging worldwide in the past decade, there has been a significant increase in multidrug-resistant bacteria from serious nosocomial infections, especially carbapenemase-producing Gram-negative bacilli that have emerged worldwide. The objective of this study is to investigate carbapenem resistance in Gram-negative bacilli bacteria using phenotypic detection, antimicrobial resistance profiles and genotypic characterisation methods. Methods 200 Gram-negative bacilli isolates were collected from different clinical specimens. All clinical samples were exposed to isolation and identification of significant pathogens applying bacteriological examination and an automated Vitek-2 system. The isolates were subjected to susceptibility tests by the Vitek-2 automated system and those isolates that were resistant to beta-lactam drugs, including carbapenems, third-generation cephalosporines or cefoxitin, were selected for phenotyping using Carba plus disc system assay for detection of carbapenemase-producing isolates. These isolates were further confirmed by molecular detection. PCR was used for the detection carbapenem-resistant genes (OXA-48, IMP, NDM, VIM, and KPC). Results 110 (55%) of 200 Gram-negative bacilli were identified as beta-lactam-resistant isolates. The frequency of carbapenem-resistant isolates was calculated to be 30.9% (n = 34/110). A collection totalling 65/110 (59%) isolates were identified as carbapenemase producers by phenotypic method. Moreover, among the 65 carbapenemase-producing Gram-negative isolates with a positive phenotype-based result, 30 (46%), 20 (30%) and 18 (27%) isolates were positive for OXA-48, KPC and MBL enzymes, respectively, as well as the production of 27% of AmpC with porin loss. Tigecycline was the most effective antibiotic that affected 70% of MDR isolates, but high rates of resistance were detected to other tested antimicrobials. Of interest, a high incidence of MDR, XDR and PDR profiles were observed among all carbapenemase-producing isolates. 36% (24/65) of the tested isolates were MDR to 3 to 5 antimicrobial classes. 29% (17/65) of the recovered isolates were XDR to 6 to 7 antimicrobial classes. Alarmingly, 24% (16/65) of isolates displayed PDR to all the tested 8 antimicrobial classes. Genotype assay, including 53 phenotypically confirmed carbapenemase-producing isolates of Gram-negative bacilli, found 51(96%) isolates were harbouring one or more genes. The most common carbapenemase gene was bla NDM 83% (44/53) followed by bla OXA-48 75% (40/53), bla VIM 49% (26/53) and bla IMP 43% (23/53), while the gene bla KPC was least frequent 7% (4/53). 92% (46/51) of isolates were involved in the production of more than one carbapenemase gene. Conclusion This study demonstrated the emergence of carbapenemase-producing Gram-negative pathogens implicated in healthcare-related infections. Accurate identification of carbapenem-resistant bacterial pathogens is essential for patient treatment, as well as the development of appropriate contamination control measures to limit the rapid spread of pathogens. Tigecycline exhibited potent antimicrobial activity against MDR, XDR and PDR-producing strains that establish a threatening alert which indicates the complex therapy of infections caused by these pathogens.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and characterisation of carbapenemase encoding genes in multidrug-resistant Gram-negative bacilli

2021

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“…When both Discs C and D show zone differences of 5mm or more compared with Disc A, AmpC production is indicated. OXA-48 production is indicated when an inhibition zone diameter of 10mm or less around Disc E as the high-level resistance to Temocillin is characteristic of OXA-48 [10]. Quality control of the culture media, Gram stain, and antimicrobial sensitivity tests was checked using standardized reference strains of E. coli (ATCC 25922), S. aureus (ATCC 25923) and P. aeruginosa ATCC 27853 [9].…”

Section:  Antimicrobial Testingmentioning

confidence: 99%

Prevalence and antimicrobial resistance patterns of nosocomial pathogens causing Surgical Site Infections in an Egyptian University hospital.

Metwally

Aamir

2020

Microbes and Infectious Diseases

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Background: Surgical site infections (SSI) are a common type of health care associated infections. The emergence of multidrug resistant (MDR) nosocomial pathogens represents a major health burden. This study was conducted to determine the frequency of isolation and patterns of antimicrobial resistance of nosocomial pathogens causing SSI in Zagazig University Hospitals Methods: Samples obtained from the infected surgical wounds were subjected to microbiological identification and antibiotic susceptibility testing. The role of extended spectrum beta lactamase (ESBL) and carbapenemase in bacterial resistance to some antibiotic were evaluated. Results: The most frequently isolated species were S. aureus (31%) followed by Klebsiella pneumoniae (K. pneumonia) (22%), Escherichia coli (E. coli) (15%), Pseudomonas aeruginosa (P. aeruginosa) (11%), Coagulase negative staphylococci (CoNs) (8%), Proteus spp (7%) and Acinetobacter spp (6%). Methicillin resistance was detected in 38 (97 %) and 8(80 %) of S. aureus and CoNs isolates, respectively. Among Gramnegative organisms, 65.8% of isolates were ESBL producers, of which 60% were Carbapenem resistant. Metallo-β-lactamase was detected in 30% of Gram-negative isolates. Multi-drug resistance was observed in 50 isolates (68.5%), whereas extensively drug resistance (XDR) occurred in 23(31.5%) of Gram-negative isolates. Conclusions: Most of Gram-negative isolates were MDR or XDR. Antibiotic therapy of SSI must be guided by microbiological culture and antibiotic sensitivity testing. Infection prevention and control practice needs more improvement. Rationalization of antibiotic prescription must be carried out. Post discharge surveillance of SSI needs to be considered.

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Multiplex lateral flow test strip for detection of carbapenemase genes using barcoded tetrahedral DNA capture probe-based biosensing interface

Xu,

Luo,

Lai

et al. 2023

Microchim Acta

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Evaluation of MASTDISCS combi Carba plus for the identification of metallo‐β‐lactamases, KPC and OXA‐48 carbapenemase genes in Enterobacteriaceae clinical isolates

Cited by 17 publications

References 17 publications

Prevalence and characterisation of carbapenemase encoding genes in multidrug-resistant Gram-negative bacilli

Prevalence and characterisation of carbapenemase encoding genes in multidrug-resistant Gram-negative bacilli

Prevalence and antimicrobial resistance patterns of nosocomial pathogens causing Surgical Site Infections in an Egyptian University hospital.

Multiplex lateral flow test strip for detection of carbapenemase genes using barcoded tetrahedral DNA capture probe-based biosensing interface

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