Evaluation of manual and automatic segmentation of the mouse heart from CINE MR images

Heijman, Edwin; Aben, Jean-Paul; Penners, Cindy; Niessen, Petra; Guillaume, René; Eys, Guillaume van; Nicolay, Klaas; Strijkers, Gustav J.

doi:10.1002/jmri.21236

Cited by 34 publications

(41 citation statements)

References 32 publications

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“…Even though MRI is often considered the gold standard for the assessment of LV volumes and function, measurements of EDV, ESV, and EF are not without error and depend on the analysis software (23,29,30). Because MRI works on a stack of 2-dimensional slices and not on a true 3-dimensional dataset, the assessment of LV shortening can be a problem.…”

Section: Discussionmentioning

confidence: 99%

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Quantification of Left Ventricular Volumes and Ejection Fraction in Mice Using PET, Compared with MRI

Stegger

Heijman²,

Schäfers³

et al. 2008

J Nucl Med

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PET has become an important noninvasive imaging technique in cardiovascular research for the characterization of mouse models in vivo. This modality offers unique insight into biochemical changes on a molecular level, with excellent sensitivity. However, morphologic and functional changes may be of equal importance for a thorough assessment of left ventricular (LV) pathophysiology. Although echocardiography and MRI are widely considered the imaging techniques of choice for the assessment of these parameters, their use with PET considerably increases study complexity and decreases cost-and time-efficiency. In this study, a novel method for the additional quantification of LV volumes and ejection fraction (EF) from PET was evaluated using cardiac MRI as the reference method. Methods: The radiolabeled glucose derivative 18 F-FDG was injected into 33 mice (6 mice with previous permanent occlusion of the left anterior descending artery [LAD], 15 mice with a temporary 30-min occlusion of the LAD, and 12 mice without previous surgery). 18 F-FDG uptake within the LV myocardium was measured using a dedicated small-animal PET scanner. After we reconstructed the images into 16 electrocardiogram (ECG)-gated frames, we determined the LV cavity volumes in end-diastole (EDV) and end-systole (ESV) and the EF using a semiautomatic segmentation algorithm based on elastic surfaces. A 6.3-T cardiac MRI examination was performed in the same animals using an ECG-triggered and respiratory-gated multislice cine sequence. The MR images were segmented with a semiautomatic algorithm using commercially available software. Results: Overall, measurements from PET agreed well with those obtained by MRI. Mean EDV and ESV were slightly overestimated by PET (86 6 43 mL and 44 6 42 mL), compared with MRI (73 6 44 mL and 41 6 46 mL); mean (6SD) EF was similar (PET, 55 6 19 mL; MRI, 54 6 18 mL). Correlation between PET and MRI was excellent for EDV (0.97) and ESV (0.96) and good for EF (0.86). The slope of the regression line was nearly perfect for EDV (0.98) and EF (1.01) and slightly below 1 for ESV (0.90), indicating a good separation of abnormal and normal values with PET. The y-intercept was above zero for EDV (15 mL) and ESV (7 mL) and near to zero for EF (0.2%). Conclusion: The quantification of LV volumes and EF in mice with PET is both efficient and accurate. This method allows for combined molecular and functional imaging of the left ventricle within a single scan, obviating additional sophisticated MRI in many cases.

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Section: Discussionmentioning

confidence: 99%

“…The cine MR images were analyzed on a slice-by-slice basis using the semiautomatic validated segmentation software CAAS MRV FARM (Pie Medical Imaging) to obtain EDV, ESV, and EF (23,24). Free papillary muscles were not included into the myocardial compartment.…”

Section: Data Processingmentioning

confidence: 99%

Quantification of Left Ventricular Volumes and Ejection Fraction in Mice Using PET, Compared with MRI

Stegger

Heijman²,

Schäfers³

et al. 2008

J Nucl Med

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“…18 LV mass was calculated by multiplying the end-diastolic myocardial wall volume with a myocardial density of 1.05 g/mL. 19 End-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), and ejection fraction (EF) were calculated as described previously.…”

Section: Image Analysismentioning

confidence: 99%

“…19 End-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), and ejection fraction (EF) were calculated as described previously. 18 Cardiac output (CO) was calculated by multiplying the SV with the average heart rate. Peak ejection rate and peak filling rate were determined from the high temporal resolution cine series as, respectively, the minimum and maximum in the time-derivative of the LV cavity volume.…”

Section: Image Analysismentioning

confidence: 99%

Fasting-Induced Myocardial Lipid Accumulation in Long-Chain Acyl-CoA Dehydrogenase Knockout Mice Is Accompanied by Impaired Left Ventricular Function

Bakermans

Geraedts

Weeghel

et al. 2011

Circ: Cardiovascular Imaging

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Background-Lipotoxicity may be a key contributor to the pathogenesis of cardiac abnormalities in mitochondrial long-chain fatty acid ␤-oxidation (FAO) disorders. Few data are available on myocardial lipid levels and cardiac performance in FAO deficiencies. The purpose of this animal study is to assess fasting-induced changes in cardiac morphology, function, and triglyceride (TG) storage as a consequence of FAO deficiency in a noninvasive fashion. Methods and Results-MRI and proton magnetic resonance spectroscopy ( 1 H-MRS) were applied in vivo in long-chain acyl-CoA dehydrogenase (LCAD) knockout (KO) mice and wild-type (WT) mice (nϭ8 per genotype). Fasting was used to increase the heart's dependency on FAO for maintenance of energy homeostasis. In vivo data were complemented with ex vivo measurements of myocardial lipids. Left ventricular (LV) mass was higher in LCAD KO mice compared with WT mice (PϽ0.05), indicating LV myocardial hypertrophy. Myocardial TG content was higher in LCAD KO mice at baseline (PϽ0.001) and further increased in fasted LCAD KO mice (PϽ0.05). Concomitantly, LV ejection fraction (PϽ0.01) and diastolic filling rate (PϽ0.01) decreased after fasting, whereas these functional parameters did not change in fasted WT mice. Myocardial ceramide content was higher in fasted LCAD KO mice compared with fasted WT mice (PϽ0.05). Conclusions-Using

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“…Few results have been reported in mice (7,8). In our experience, user correction of errors arising from automated analysis requires that user interaction be intrinsic, intuitive, and minimal.…”

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confidence: 99%

Fast left ventricular mass and volume assessment in mice with three‐dimensional guide‐point modeling

Young

Barnes

Davison

et al. 2009

Magnetic Resonance Imaging

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Purpose: To investigate the accuracy (vs. standard manual analysis) and precision (scan-rescan reproducibility) of three-dimensional guide-point modeling (GPM) for the assessment of left ventricular (LV) function in mice. Methods:Six male wildtype C57/Bl6 mice (weight 26.2 Ϯ 1.1 g) were scanned twice, 3 days apart. Each scan was performed twice, at 0.2 mm/pixel with one average and at 0.1 mm/pixel with two averages. The 24 studies were anonymized and analyzed in blinded fashion using GPM and standard manual slice summation. Results:The average error between GPM and standard analysis was 2.3 Ϯ 5.8 mg in mass, 1.7 Ϯ 3.2 L in enddiastolic volume, 2.3 Ϯ 3.1 L in end-systolic volume, Ϫ2.7 Ϯ 4.3% in ejection fraction, Ϫ0.6 Ϯ 3.3 L in stroke volume, and Ϫ0.31 Ϯ 1.56 ml ⅐ min Ϫ1 in cardiac output (mean difference Ϯ SD of differences, n ϭ 24). The average time taken was 8.0 Ϯ 2.5 minutes for 3D GPM and 48.5 Ϯ 8.9 minutes for standard analysis (n ϭ 24). Scan-rescan reproducibility results were similar to the standard analysis. No significant differences were found using linear mixed effects modeling in either accuracy or precision between scan resolutions or analysis method. Conclusion:3D GPM enables fast analysis of mouse LV function, with similar accuracy and reproducibility to standard analysis. An image resolution of 0.2 mm/pixel with one average is adequate for LV function studies.

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Evaluation of manual and automatic segmentation of the mouse heart from CINE MR images

Cited by 34 publications

References 32 publications

Quantification of Left Ventricular Volumes and Ejection Fraction in Mice Using PET, Compared with MRI

Quantification of Left Ventricular Volumes and Ejection Fraction in Mice Using PET, Compared with MRI

Fasting-Induced Myocardial Lipid Accumulation in Long-Chain Acyl-CoA Dehydrogenase Knockout Mice Is Accompanied by Impaired Left Ventricular Function

Fast left ventricular mass and volume assessment in mice with three‐dimensional guide‐point modeling

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