Evaluation of Mannitol Intervention Effects on Ischemic Cerebral Edema in Mice Using Swept Source Optical Coherence Tomography

Abstract

Cerebral edema is a serious complication of ischemic cerebrovascular disease and mannitol is a commonly used dehydrating agent for relieving cerebral edema. However, the edema state and surrounding vascular perfusion level during mannitol treatment remains unclear, which affects the clinical application of the medicine. In this paper, we demonstrated the role of swept-source optical coherence tomography (OCT) in the evaluation of mannitol efficacy using mouse models. The OCT-based angiography and attenuation imaging technology were used to obtain the cerebral vascular perfusion level and cerebral edema state at different times. Vascular parameters and edema parameters were quantified and compared. Experimental results show that mannitol can significantly reduce the water content in the central region of edema, effectively inhibiting the rapid growth of the edema area, and restoring cerebral blood flow. On average, the edema area decreased by 33% after 2 h, and the vascular perfusion density increased by 12% after 5 h. This work helps to provide a valuable theoretical basis and research ideas for the clinical treatment of cerebral edema.

Multimodal OCT Control for Early Histological Signs of Vulvar Lichen Sclerosus Recurrence after Systemic PDT: Pilot Study

“…From a histological point of view, this is due to both the homogenization of the dermis ( Figure 2 A) and the formation of edema in severe VLS [ 35 ]. A similar effect was observed by other researchers in cerebral edema [ 41 ].…”

Probe fusion all-optic OCT-PAM dual-mode imaging system for biomedical imaging

“…8 (e-h). Based on the binarized image, three vascular parameters were quantified to evaluate the blood flow status, including vascular perfusion density (VPD), vascular diameter (VD) and vascular length (VL) [46] . VPD was defined as the ratio of "the number of blood vessel pixels" to "the total number of pixels in the angiogram".…”

“…Unlike PAM, the contrast provided by OCT is generated through the scattering of light within the tissue microstructure. In addition to the initial structural imaging, OCT has developed several extended functions, including DOCT [4] , OCTA [6] , and OCT attenuation imaging (OCT-AI) [46] , etc. The capacity of OCT and PAM to offer complementary information renders them the ideal modalities for multimodal imaging systems.…”