Evaluation of MALDI Biotyper Interpretation Criteria for Accurate Identification of Nontuberculous Mycobacteria

Rodríguez-Temporal, David; Rodríguez‐Sánchez, Belén; Alcaide, Fernando

doi:10.1128/jcm.01103-20

Cited by 29 publications

(35 citation statements)

References 28 publications

(30 reference statements)

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“…Mycobacterial misidentifications by MALDI-TOF MS have been reported in literature, even with high log(score) 20 . Fortunately, this is not a common phenomenon.…”

Section: Discussionmentioning

confidence: 98%

“…In summary, MALDI-TOF MS demonstrated an extraordinary inter-laboratory reliability for the identification of NTMs, reaching a high concordance among identifications obtained in participating centres. Besides, sonication of the bacterial pellet has shown to be a good alternative to the MycoEX procedure 20 . Misidentifications were scarce and they were considered as minor errors since the identifications provided by the MALDI-TOF MS corresponded to species from the same complex.…”

Section: Discussionmentioning

confidence: 99%

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Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

Rodríguez-Temporal

Alcaide

Mareković

et al. 2022

Sci Rep

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The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2–98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.

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“…Mycobacterial misidentifications by MALDI-TOF MS have been reported in literature, even with high log(score) 20 . Fortunately, this is not a common phenomenon.…”

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

Rodríguez-Temporal

Alcaide

Mareković

et al. 2022

Sci Rep

Self Cite

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“…Therefore, the rapid detection and identification of NTM species is very important for the diagnosis and treatment of NMLD. At present, most of the reported NTM strain identification methods are based on cultures to obtain isolates before strain identification (10)(11)(12). Due to the long cultivation time of mycobacteria, it takes 1-8 weeks and sometimes as long as 12 weeks to obtain a culture (13).…”

Section: Discussionmentioning

confidence: 99%

The value of gene chip detection of bronchoalveolar lavage fluid in the diagnosis of nontuberculous mycobacterial lung disease

Huang¹,

Lin²,

Chen³

et al. 2021

Ann Palliat Med

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Background: Nontuberculous mycobacteria (NTM) are mycobacteria other than mycobacterium tuberculosis complex (MTBC) and mycobacterium leprae. NTM can cause infection in many human tissues and organs and is most commonly seen in the lungs. Clinically, the symptoms and signs of nontuberculous mycobacteria lung disease (NMLD) are very similar to those of tuberculosis (TB). Because most NTMs are resistant to conventional anti-TB drugs, the rapid diagnosis of NMLD is the key to treatment. This study aimed to use gene chip technology to examine bronchoalveolar lavage fluid (BALF) from NMLD patients to explore the value of this technique for the rapid diagnosis of NMLD in BALF.Methods: A retrospective analysis of 308 patients with NMLD treated at Fuzhou Pulmonary Hospital from January 2018 to June 2020 was performed. BALF was collected from the patients. Gene chip detection (Capital Bio Corporation, Chengdu, China) and BACTEC MGIT960 (Becton, Dickinson and Company, MD, USA) liquid culture were performed to compare the NTM positive detection rates between the two methods. The NTM strain isolated from liquid culture were identified by rDNA sequencing and the results of identification were compared with those of gene chip detection using BALF specimens.Results: A total of 221 cases of NTM were detected in 308 BALF specimens by the gene chip method; the positive rate was 71.75% (221/308). A total of 218 cases of NTM were detected by the liquid culture method, and the positive rate was 70.78% (218/308). There was no significant difference in the positive rate of NTM detected in BALF specimens between the two methods (χ 2 =0.138 P=0.804>0.05); 187 cases were detected with both sequencing and gene chip detection, and the coincidence rate of strain identification with the two methods reached 96.79% (181/187). Sequencing of 218 strains of NTM was carried out; eight species were identified, and the top four species were M. intracellulare (131/218,60.09%), M. avium (48/218,22.02%), M. abscessus (27/218,12.38%), and M. kansasii (5/218,2.29%). Conclusions: Gene chip technology can rapidly detect NTM in BALF and accurately identify bacterial species. It has important clinical value in the early diagnosis and treatment of NMLD.

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“…For the Bruker systems, MALDI-TOF scores higher or equal to 2.0 are required for a high confidence identification. Nevertheless, due to the biological features of mycobacteria, previous studies have shown that lower scores can effectively be used for NTM identification (15,26). Therefore, MALDI-TOF scores higher or equal to 1.8 were considered high confidence identifications as described elsewhere (26) and were used to establish a positive NTM identification.…”

Section: Protein Extraction and Maldi-tof Ms Analysismentioning

confidence: 99%

“…However, due to the increased costs and technical and training requirements of WGS, its implementation in routine diagnosis of NTM is limited. As a result, many laboratories still use a combination of probes and Sanger sequencing as a reference method to identify NTM species (8,15,16).…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF Mass Spectrometry as a Rapid Screening Alternative for Non-tuberculous Mycobacterial Species Identification in the Veterinary Laboratory

et al. 2022

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Non-tuberculous mycobacteria (NTM) are difficult to identify by biochemical and genetic methods due to their microbiological properties and complex taxonomy. The development of more efficient and rapid methods for species identification in the veterinary microbiological laboratory is, therefore, of great importance. Although MALDI-TOF Mass Spectrometry (MS) has become a promising tool for the identification of NTM species in human clinical practise, information regarding its performance on veterinary isolates is scarce. This study assesses the capacity of MALDI-TOF MS to identify NTM isolates (n = 75) obtained from different animal species. MALDI-TOF MS identified 76.0% (n = 57) and 4% (n = 3) of the isolates with high and low confidence, respectively, in agreement with the identification achieved by Sanger sequencing of housekeeping genes (16S rRNA, hsp65, and rpoB). Thirteen isolates (17.3%) were identified by Sanger sequencing to the complex level, indicating that these may belong to uncharacterised species. MALDI-TOF MS approximated low confidence identifications toward closely related mycobacterial groups, such as the M. avium or M. terrae complexes. Two isolates were misidentified due to a high similarity between species or due to the lack of spectra in the database. Our results suggest that MALDI-TOF MS can be used as an effective alternative for rapid screening of mycobacterial isolates in the veterinary laboratory and potentially for the detection of new NTM species. In turn, Sanger sequencing could be implemented as an additional method to improve identifications in species for which MALDI-TOF MS identification is limited or for further characterisation of NTM species.

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Evaluation of MALDI Biotyper Interpretation Criteria for Accurate Identification of Nontuberculous Mycobacteria

Cited by 29 publications

References 28 publications

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

The value of gene chip detection of bronchoalveolar lavage fluid in the diagnosis of nontuberculous mycobacterial lung disease

MALDI-TOF Mass Spectrometry as a Rapid Screening Alternative for Non-tuberculous Mycobacterial Species Identification in the Veterinary Laboratory

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