Evaluation of maize inbred Iines for resistance to sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV)

Kuntze, L.; Fuchs, E.; Grüntzig, Maria; Schulz, Britta; Henning, U.; Hohmann, F.; Melchinger, A. E.

doi:10.1051/agro:19950714

Cited by 24 publications

(17 citation statements)

References 6 publications

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“…Investigation on resistant character of maize was initially conducted in USA against MDMV, a relative virus to SCMV (Kuntze et al 1995). Most experimental results were obtained from the same material inbred line Pa405, but the results showed variation in genetic basis according to different reports.…”

Section: Introductionmentioning

confidence: 99%

Molecular mapping of a major QTL conferring resistance to SCMV based on immortal RIL population in maize

2009

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Section: Introductionmentioning

confidence: 99%

Molecular mapping of a major QTL conferring resistance to SCMV based on immortal RIL population in maize

2009

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“…According to Louie (1986) and Kuntze et al (1995), mechanical inoculation was done twice within one week at the third to the fourth leaf stages, using sap from maize plants systemically infected by MDMV as inoculums. The disease incidence and symptom scale were investigated at the adult stage according to the standard proposed by Lin (1989).…”

Section: Virus Resistance Evaluationmentioning

confidence: 99%

Improvement of resistance to maize dwarf mosaic virus mediated by transgenic RNA interference

Zhang

Yang

Zhou

et al. 2011

Journal of Biotechnology

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“…On the basis of reports by Louie (1986) and Kuntze et al (1995), mechanical inoculation was done twice within 1 week at the three-to four-leaf stage, using inoculum prepared from …”

Section: Field Inoculated Evaluationmentioning

confidence: 99%

RNA Interference-Based Transgenic Maize Resistant to Maize Dwarf Mosaic Virus

Zhang

Gou

et al. 2010

J. Plant Biol.

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Maize dwarf mosaic virus (MDMV) is a widespread pathogenic virus that causes serious loss of yield in maize (Zea mays). RNA interference (RNAi) triggered by hairpin RNA (hpRNA) transcribed from a transgenic inverted-repeat sequence is an effective way to defend against viruses in plants. In this study, an hpRNA expression vector containing a sense arm and an antisense arm of 150 bp separated by an intron of the maize actin gene was constructed to target the P1 protein (protease) gene of MDMV and used to transform Agrobacterium tumefaciens strain EHA105. The transformed Agrobacterium strain was used to transform maize embryonic calli isolated from immature embryos by an improved culture technique. In all, 46 plants were regenerated after stringent hygromycin B selection, and 18 of them were certified to be positive by PCR amplification. Of these positive plants, 13 were grown to produce offspring, and nine were identified by Southern blotting to have the transgene integrated with one or two copies. The resistance of three T 2 lines was evaluated in a field trial of dual MDMV inoculation in two environments and was found to be improved compared with the non-transformed control. The disease indexes of the transgenic plant lines h2, 13, and h1 were not significantly different from the highly resistant control line H9-21. The viral titers of the inoculated plants were detected by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), and the result was in accord with the resistance evaluated in the field trial. The addition of uniconazole S3307 (0.25 mgl −1 ) and ABT rootpromoting powder (0.5 mg l −1 ) showed a significant improvement of hardening in regenerated plantlets, which were stronger and generated a better fibrous root system than the control. This improvement could facilitate the transgenic operation of maize.

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Evaluation of maize inbred Iines for resistance to sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV)

Cited by 24 publications

References 6 publications

Molecular mapping of a major QTL conferring resistance to SCMV based on immortal RIL population in maize

Molecular mapping of a major QTL conferring resistance to SCMV based on immortal RIL population in maize

Improvement of resistance to maize dwarf mosaic virus mediated by transgenic RNA interference

RNA Interference-Based Transgenic Maize Resistant to Maize Dwarf Mosaic Virus

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