Evaluation of Lymphocyte Subpopulations in Cord Blood of Bulgarian Newborns

Normal B-cell ranges in infants: A systematic review and meta-analysis

“…Of the 37 studies included in this SLR, 36 had an observational design, 5,[49][50][51] and 1 was a non-randomized study 48 with different feeding interventions (human milk vs fortified human milk vs formula). The geographic distribution covered Europe (20 studies), 5,17,18,20,22,26,27,29,31,32,34,36,38,40,42,43,[47][48][49]51 North America (6 studies), 16,19,35,37,44,46 Asia (5 studies), 23,30,33,45,50 Africa (4 studies), 24,25,31,41 South America (2 studies) 21,39 and Australasia (1 study) (see Table E3 in the Online Repository at www.jacionline.org). 28 The study populations included healthy infants in all cases, often defined as infants with no history of immunologic or hematologic diseases (see Table E3).…”

“…The most common gating strategy was forward and side scatter (16 studies), 5,16,[18][19][20][21][22]25,28,33,[35][36][37]46,47,49 in which case visible light scatter is measured in the forward and perpendicular directions to capture both the relative size and complexity of the cell. 53 Among the remaining studies, 7 used CD45 versus side scatter 17,23,24,26,34,38,44 ; for 14 studies, details of the gating strategy were unclear or not reported (see Tables E4 and E5 in the Online Repository at www.jacionline.org). 27,[29][30][31][32][39][40][41][42][43]45,48,50,51 Most articles (>70%) did not provide specific details about number of lymphocyte events collected or required for analysis; in the 10 articles that did provide this information, the number of events stated ranged from 6,500 to 10,000 (see Table E3).…”