Evaluation of low-cost SARS-CoV-2 RNA purification methods for viral quantification by RT-qPCR and next-generation sequencing analysis: Implications for wider wastewater-based epidemiology adoption

Reyes-Calderón, Alonso; Mindreau-Ganoza, Elías; Pardo-Figueroa, Braulio; Garcia-Luquillas, Katherine  R.; Yufra, Sonia P.; Romero, Pedro E.; Antonini, Claudia; Renom, Jose-Miguel; Mota, César R.; Santa-María, Mónica C.

doi:10.1016/j.heliyon.2023.e16130

Heliyon

2023

DOI: 10.1016/j.heliyon.2023.e16130

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Evaluation of low-cost SARS-CoV-2 RNA purification methods for viral quantification by RT-qPCR and next-generation sequencing analysis: Implications for wider wastewater-based epidemiology adoption

Alonso Reyes-Calderón

Elías Mindreau-Ganoza

Braulio Pardo-Figueroa

et al.

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2024

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Identifying spatiotemporal trends of SARS-CoV-2 RNA in wastewater: from the perspective of upstream and downstream wastewater-based epidemiology (WBE)

Tran,

You,

Liu

et al. 2024

Environ Sci Pollut Res

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Recently, many efforts have been made to address the rapid spread of newly identi ed COVID-19 virus variants . Wastewater-based epidemiology (WBE) is considered as a potential early warning tool for identifying the rapid spread of this virus. This study investigated the occurrence of SARS-CoV-2 in eight wastewater treatment plants (WWTPs) and their sewerage systems which serve most of the population in Taoyuan City, Taiwan. Across the entire study period, the wastewater viral concentrations were correlated with the number of COVID-19 cases in each WWTP (Spearman' r = 0.23 -0.76). In addition, it is con rmed that several treatment technologies could effectively eliminate the virus RNA from WWTPs in uent (> 90 %). On the other hand, further results revealed that an inverse distance weighted (IDW) interpolation and hot spot model combined with geographic information system (GIS) method could be applied to analyze the spatiotemporal variations of SARS-CoV-2 in wastewater from sewer system. In addition, socioeconomic factors namely population density, land-use, and tax-income were successfully identi ed as the potentials drivers which substantially affect the onset of COVID-19 outbreak in Taiwan. Finally, the data obtained from this study can provide a powerful tool in public health decision-making not only in response to the current epidemic situation but also other epidemic issues in the future.

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Identifying spatiotemporal trends of SARS-CoV-2 RNA in wastewater: from the perspective of upstream and downstream wastewater-based epidemiology (WBE)

Tran,

You,

Liu

et al. 2024

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

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Evaluation of low-cost SARS-CoV-2 RNA purification methods for viral quantification by RT-qPCR and next-generation sequencing analysis: Implications for wider wastewater-based epidemiology adoption

Cited by 1 publication

References 75 publications

Identifying spatiotemporal trends of SARS-CoV-2 RNA in wastewater: from the perspective of upstream and downstream wastewater-based epidemiology (WBE)

Identifying spatiotemporal trends of SARS-CoV-2 RNA in wastewater: from the perspective of upstream and downstream wastewater-based epidemiology (WBE)

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