Evaluation of Loop-Mediated Isothermal Amplification Assay for Detection and Typing of Human Papilloma Virus 16 and 18 from Endocervical Samples

Mudhigeti, Nagaraja; Kalawat, Usha; Hulikal, Narendra; Kante, Meenakshi

doi:10.4103/ijmm.ijmm_19_58

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…LAMP technique uses Bst polymerase and four to six primers to rapidly amplify either DNA or RNA (under 1 h) at elevated temperatures between 55°C and 70°C. It is commonly used for amplification of bacterial and viral nucleic acids, and thus HPV has been targeted by LAMP in various studies 60–73 . Despite its advantages, LAMP is highly sensitive to contamination (similarly to PCR), requiring ideally sterile DNA‐free and RNA‐free workspace.…”

Section: Isothermal Amplification Techniques As Rapid Alternatives To...mentioning

confidence: 99%

“…for amplification of bacterial and viral nucleic acids, and thus HPV has been targeted by LAMP in various studies. [60][61][62][63][64][65][66][67][68][69][70][71][72][73] Despite its advantages, LAMP is highly sensitive to contamination (similarly to PCR), requiring ideally sterile DNA-free and RNA-free workspace.…”

Section: Isothermal Amplification Techniques As Rapid Alternatives To...mentioning

confidence: 99%

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Advanced technologies towards improved HPV diagnostics

Bartosik,

Moranova,

Izadi

et al. 2024

Journal of Medical Virology

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Persistent infection with high‐risk types of human papillomaviruses (HPV) is a major cause of cervical cancer, and an important factor in other malignancies, for example, head and neck cancer. Despite recent progress in screening and vaccination, the incidence and mortality are still relatively high, especially in low‐income countries. The mortality and financial burden associated with the treatment could be decreased if a simple, rapid, and inexpensive technology for HPV testing becomes available, targeting individuals for further monitoring with increased risk of developing cancer. Commercial HPV tests available in the market are often relatively expensive, time‐consuming, and require sophisticated instrumentation, which limits their more widespread utilization. To address these challenges, novel technologies are being implemented also for HPV diagnostics that include for example, isothermal amplification techniques, lateral flow assays, CRISPR‐Cas‐based systems, as well as microfluidics, paperfluidics and lab‐on‐a‐chip devices, ideal for point‐of‐care testing in decentralized settings. In this review, we first evaluate current commercial HPV tests, followed by a description of advanced technologies, explanation of their principles, critical evaluation of their strengths and weaknesses, and suggestions for their possible implementation into medical diagnostics.

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Section: Isothermal Amplification Techniques As Rapid Alternatives To...mentioning

confidence: 99%

Section: Isothermal Amplification Techniques As Rapid Alternatives To...mentioning

confidence: 99%

Advanced technologies towards improved HPV diagnostics

Bartosik,

Moranova,

Izadi

et al. 2024

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“… Pathogen Time Sensitivity Detection Method References Zika 20 min 10 −5 PFU Colorimetric ( Calvert et al., 2017 ) Ebola 15–20 min 256 copies Real-time fluorometer ( Oloniniyi et al., 2017 ) HIV 60 min 10 4 RNA copies/mL RT Quencher probe ( Rudolph et al., 2015 ) Influenza 60 min 0.1 RNA copies Phenol-red pH indicator ( Ahnet al. et al., 2019 ) Hepatitis B 15 min 2.2fg/µl Fluorometer ( Quoc et al., 2018 ) Dengue 15 min 0.8 fg/µl Colorimetric (SYBR Green I) ( Mendes et al., 2019 ) HPV 60 min 10 copies Colorimetric (SYBR Green I) ( Mudhigeti et al., 2019 ) H1N1 <40 min 10 copies RNA Real-time turbidimeter, UV ( Kubo et al., 2010 ) Encephalitis 70 min 50 pg RNA Lateral flow dipstick ( Deng et al., 2015 ) MERS-CoV 60 min 4 RNA copies Colorimetric (EvaGreen) ( Lee et al., 2017 ) West Nile virus 17 min 0.1 PFU Real-time turbidimeter ( Parida et al., 2004 ) …”

Section: Reverse Transcription Lamp (Rt-lamp)mentioning

confidence: 99%