Evaluation of lncRNA Expression Pattern and Potential Role in Heart Failure Pathology

Abstract

During the last few decades, the morbidity and mortality of heart failure (HF) have remained on an upward trend. Despite the advances in therapeutic and diagnostic measures, there are still many aspects requiring further research. This study is aimed at finding potential long noncoding RNAs (lncRNAs) that could aid with the diagnosis and treatment of HF. We performed RNA sequencing on the peripheral blood of healthy controls as well as HF patients. The expression of lncRNAs was validated by RT-qPCR. Bioinformatic analysis was performed to investigate the possible mechanism of differentially expressed lncRNAs and mRNAs. The diagnostic value of lncRNAs was analysed by ROC analysis. Finally, a total of 207 mRNAs and 422 lncRNAs were identified. GO and KEGG pathway analyses revealed that biological pathways such as immune response, regulation of cell membrane, and transcriptional regulatory process were associated with the pathological progress of HF. The lncRNA-mRNA coexpression network was conducted, and several mRNAs were identified as key potential pathological targets, while lncRNA CHST11, MIR29B2CHG, CR381653.1, and FP236383.2 presented a potential diagnostic value for HF. These findings provide novel insights for the underlying mechanisms and possible therapeutic targets for HF.

Transcriptomics analysis of long non-coding RNAs in smooth muscle cells from patients with peripheral artery disease and diabetes mellitus

“…Five signi cantly differentially expressed lncRNAs between our study groups with and without DM, namely NEAT1, XIST, MIR222HG, MIR100HG, HIF1A-AS3, and MIR29B2CHG, have already been associated with CVDs 30,[39][40][41][42][43][44][45][46] , mainly with the proliferation, migration, apoptosis, and phenotypic switch of SMCs (Table 3) 39,44,45,[47][48][49] .…”

“…However, because the other isoforms affect the behaviour of SMCs, it can be assumed that HIF1A-AS3, signi cantly downregulated in the DMplus study group, might also affect the function of SMCs. The lncRNAs MIR100HG and MIR29B2CHG have only been mentioned in the context of cardiomyopathy and heart failure 40,46 . So far, there is no information about their potential role in PAD, DM or SMCs.…”

Transcriptomics analysis of long non-coding RNAs in smooth muscle cells from patients with peripheral artery disease and diabetes mellitus

“…However, because the other isoforms affect the behaviour of SMCs, it can be assumed that HIF1A-AS3 , significantly downregulated in the DMplus study group, might also affect the function of SMCs. The lncRNAs MIR100HG and MIR29B2CHG have only been mentioned in the context of cardiomyopathy and heart failure 42 , 48 . So far, there is no information about their potential role in PAD, DM or SMCs.…”