Evaluation of Liver Toxicological Effects Induced by Polyalkylcyanoacrylate Nanoparticles

Fernández-Urrusuno, Rocío; Fattal, Elias; Porquet, Dominique; Féger, Jeanne; Couvreur, Patrick

doi:10.1006/taap.1995.1032

Cited by 50 publications

(15 citation statements)

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“…Measurements were made up to eight months after NP synthesis. Our findings related to particle size are in agreement with reports from several other authors that have submitted NPs to similar conditions [19][20][21][22].…”

Section: Nps Characterizationsupporting

confidence: 96%

Three-Dimensional Systems in Polybutylcyanoacrylate Nanoparticles Safety Evaluation

Santos

Tiago

Maria‐Engler

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

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It is generally accepted that three-dimensional (3D) cell culture systems better represent cell physiology and morphology than two-dimensional (monolayer) cell cultures. The aim of this work was to develop a 3D model that could efficiently evaluate polymeric materials biocompatibility using polybutylcyanoacrylate nanoparticles (PBCA-NP). Normal human fibroblasts and melanoma cells were cultured both in monolayer and on type I collagen matrix. Trypan blue and MTT assays were used to evaluate PBCA-NP cytotoxicity, which was shown to be dose-and time-dependent to all cell types. These results were confirmed by cell death analysis with flow cytometry. There is evidence that treatment with PBCA-NP in concentrations close to the IC 50 inhibit autophagy in fibroblasts and melanoma cells when cultured in monolayer, but this response could not be observed in the 3D system. These findings strongly suggest that PBCA-NP can modify cell response depending on the concentration used and the conditions of cell culture.

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Section: Nps Characterizationsupporting

confidence: 96%

Three-Dimensional Systems in Polybutylcyanoacrylate Nanoparticles Safety Evaluation

Santos

Tiago

Maria‐Engler

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

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“…It has been demonstrated that, after intravenous administration, biodegradable polyalkylcyanoacrylate or poly (lactide-co-glycolide) (PLGA) nanoparticles can trigger inflammation and, in the case of poly (alkylcyanoacrylate), oxidative stress during both acute and chronic treatments. [17][18][19] However, these effects were reversible after interruption of the treatment, which was not the case for non-biodegradable polystyrene nanoparticles. Nevertheless, the question about a similar deleterious effect on the lung remains open, and the goal of the present paper is to answer this question.…”

Section: Introductionmentioning

confidence: 99%

Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells

Mura¹,

Hillaireau²,

Nicolas³

et al. 2011

IJN

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Background Because of the described hazards related to inhalation of manufactured nanoparticles, we investigated the lung toxicity of biodegradable poly (lactide-co-glycolide) (PLGA) nanoparticles displaying various surface properties on human bronchial Calu-3 cells. Methods Positively and negatively charged as well as neutral nanoparticles were tailored by coating their surface with chitosan, Poloxamer, or poly (vinyl alcohol), respectively. Nanoparticles were characterized in terms of size, zeta potential, and surface chemical composition, confirming modifications provided by hydrophilic polymers. Results Although nanoparticle internalization by lung cells was clearly demonstrated, the cytotoxicity of the nanoparticles was very limited, with an absence of inflammatory response, regardless of the surface properties of the PLGA nanoparticles. Conclusion These in vitro results highlight the safety of biodegradable PLGA nanoparticles in the bronchial epithelium and provide initial data on their potential effects and the risks associated with their use as nanomedicines.

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“…Toriumi et al [5] determined that Histoacryl (butyl-2-cyanoacrylate) caused minimal if any inflammation when small amounts of glue was used in the nonvascular region between bone graft and cartilage; however, subcutaneous implantation contacting well-vascularized soft tissue resulted in increased acute inflammation and prolonged foreign-body giant-cell response. Fernandez-Urrusuno et al [6] determined that chronic intravenous administration of drug-loaded polyalkylcyanoacrylate nanoparticles led to hepatotoxicity, which was reversible when the treatment was stopped. Upon contact, toxicity may manifest itself as urticaria, contact dermatitis and other dermatoses [1].…”

Section: Discussionmentioning

confidence: 98%

Accidental cyanoacrylate glue ingestion

Yılmaz

2005

International Journal of Pediatric Otorhinolaryngology

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Evaluation of Liver Toxicological Effects Induced by Polyalkylcyanoacrylate Nanoparticles

Cited by 50 publications

References 0 publications

Three-Dimensional Systems in Polybutylcyanoacrylate Nanoparticles Safety Evaluation

Three-Dimensional Systems in Polybutylcyanoacrylate Nanoparticles Safety Evaluation

Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells

Accidental cyanoacrylate glue ingestion

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