Evaluation of liquid chromatography–negative ion electrospray mass spectrometry for the determination of selected resin acids in river water

Overview of Analytical Procedures for Fatty and Resin Acids in the Papermaking Process

“…In addition, the use of acetonitrile caused high background noise, since carbon deposits were produced in the corona of the APCI interface. Dehydroabietic acid was able to separate from the non-aromatic acids that coeluted in the McMartin et al (2002) study. On the other hand, Rigol et al (2003b) doubled the analysis sensitivity by using APCI and partly separating the coeluting nonaromatic resin acids with a C8 column and adding isopropanol in the mobile phase.…”

“…However, only limited information on HPLC-MS has been reported regarding the analysis of fatty and resin acids in papermaking waters, effluents, or river water samples (McMartin et al 2002;Latorre et al 2003;Rigol et al 2003bRigol et al , 2004Valto et al 2011). Due to the high sensitivity and selectivity of HPLC-MS, the main advantage of analysis is that samples can be directly injected into a column without the need for a derivatization step and that ionization of the compounds takes place in an interface without the need for any post-column addition.…”

“…However, compromises between chromatographic separation and ionization efficiencies must often be made when selecting the eluent composition. McMartin et al (2002) reported the use of the liquid chromatographyelectrospray-mass spectrometric (LC-ESI-MS) method for the analysis of four resin acids in river water. The method used external standard calibration and provided a highly sensitive analysis of dehydroabietic acid and coelution of three structural resin acid isomers (abietic, isopimaric, and pimaric acids).…”

“…This is necessary if, for example, detailed structural information is needed about all abietic acid isomers. The fast HPLC-MS method is suitable for individual fatty and resin acids (McMartin et al 2002;Valto et al 2011). The possible co-eluting compounds from HPLC-MS analysis can be confirmed by HPLC combined with tandem mass spectrometric detection (MS/MS).…”

Rapid Analysis of Abietanes in Conifers

“…Reversed-phase HPLC of underivatized resin acids in river water does not resolve the structural isomers studied (abietic, isopimaric, and pimaric), and detection by negative ion electrospray mass spectrometry does not provide isomeric speciation (McMartin et al, 2002). Determination of dehydroabietic and abietic acids in Chinese medications (Lee et al, 1997) and adhesive (Lee et al, 1994) with UV detection is reported but without analysis for other abietanes.…”