Evaluation of lipofuscin-like pigments as an index of lead-induced oxidative damage in the brain

Patková, Jana; Vojtísek, M; Tůma, Jan; Vožeh, F; Knotková, Jana; Santorova, Pavlina; Wilhelm, J.

doi:10.1016/j.etp.2010.06.005

Cited by 18 publications

(8 citation statements)

References 27 publications

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“…The LFLP fluorescence spectra of BSA–ALEs were measured on an F‐2000 fluorescence spectrophotometer (Hitachi, Tokyo, Japan), as described by Patková et al . The 3D contour map showed a better overview of the maximum excitation and emission wavelengths for the LFLP fluorescence spectra, which were measured in the range of 300–410 nm for the excitation wavelength and between 320 and 550 nm for the emission wavelength.…”

Section: Methodsmentioning

confidence: 99%

Three different active aldehydes induce the production of advanced lipoxidation end products upon incubation with bovine serum albumin

Gao

et al. 2015

Euro J Lipid Sci & Tech

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Accumulation of advanced lipoxidation end products (ALEs) has been implicated in many chronic and degenerative diseases. In this study, we evaluated the effects of malondialdehyde (MDA), trans-2-heptenal, and trans,trans-2,4-heptadienal on the properties of native proteins and the formation of bovine serum albumin (BSA)-ALEs. Incubation of BSA with these aldehydes resulted in modification of Lys and Arg residues, conformational changes, and formation of colored, fluorescent, lipofuscin-like pigments (exhibiting strong lipofuscin-like fluorescence for MDA-BSA, but much weaker fluorescence for the other two aldehyde-BSA molecules). Moreover, the generated BSA-aldehyde complexes exhibited ultraviolet-visible (UV-Vis) absorption, i.e., the MDA-BSA adduct exhibited increased UV-Vis absorbance at 290 and 400 nm, while the other two aldehyde-BSA molecules exhibited increased UV-Vis absorbance at 280 and 300-350 nm, respectively, and cross-linking, similar to the characteristics of Maillard reaction products. The formation of aggregates was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The variables measured above showed that heptadienal-BSA and heptenal-BSA samples exhibited both similarities and differences from MDA-BSA samples. These results provide insights into the characteristics and formation of ALEs in terms of their biochemical interactions and applications in food science.Practical applications: Advanced lipoxidation end products (ALEs) are known to be involved in many chronic and degenerative diseases. Here, we propose suitable protocols to characterize and compare ALEs derived from different secondary products of lipid oxidation. These results enhance our knowledge of the characteristics of ALEs and contribute to our understanding of the structure and formation of ALEs derived from different lipid oxidation products. Additionally, our results provide important insights into appropriate preventive or therapeutic measures to inhibit or reduce ALEs.

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Section: Methodsmentioning

confidence: 99%

Three different active aldehydes induce the production of advanced lipoxidation end products upon incubation with bovine serum albumin

Gao

et al. 2015

Euro J Lipid Sci & Tech

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“…There are several studies showing that during oxidative stress with different oxidizing agents biological molecules are damaged and converted into lipofuscin-or age pigment-like fluorescent products, which were used as indicators of oxidative damage to cells [10,14,15,25]. The fluorescent pigments generated in such reactions are usually a complex mixture of distinct compounds with the same or similar fluorescence properties.…”

Section: Discussionmentioning

confidence: 99%

“…Fluorescent lipofuscin-like pigments have been used in many studies as indicators of oxidative stress, which contributes to damage and functional loss of biological macromolecules, mainly in long-lived post-mitotic cells such as cardiac myocytes and neurons [8][9][10][11][12][13]. There are studies that have analyzed production of LFP in erythrocytes after peroxidation of their membrane lipids in order to characterize these fluorescent compounds [14,15].…”

Section: Introductionmentioning

confidence: 99%

HPLC Separation of Fluorescent Products of Lipid Peroxidation in Erythrocytes and Mitochondria

et al. 2011

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Polyunsaturated fatty acids found in membrane phospholipids are readily oxidized by free radicals producing highly reactive aldehydes such as malondialdehyde. These aldehydes react with biological material to form fluorescent lipid peroxidation end products known as lipofuscin-like pigments. We studied fluorescent pigments from beef heart mitochondria incubated with malondialdehyde in vitro and from erythrocytes of patients with Alzheimer's disease to evaluate their potential as markers of oxidative stress and to develop HPLC methods for their qualitative analysis. We used tridimensional fluorescence spectral arrays and synchronous fluorescence spectra in connection with HPLC separation with fluorescence detection. Stable fluorophores were found in both models and were successfully resolved into several distinctive fractions. This creates the basis for further characterization of this relatively less studied group of products of oxidative damage.

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“…The presence of these fluorophores might be a marker of the side effect of maternal treatment with drug on development of the bone matrix. Earlier physiological intracellular accumulation of lipofuscins in the central nervous system with ageing as well as in response to pathological conditions or to toxic compounds were reported [34]. Autofluorescence of lipofuscin in retinal pigment epithelium due to Stargardt’s disease and age-related macular degeneration [35, 36] was also discussed.…”

Section: Discussionmentioning

confidence: 99%

Prenatal Zidovudine Treatment Modifies Early Development of Rat Osteoid – Confocal Microspectroscopy Analysis

2019

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Autofluorescence of the bone extracellular matrix (ECM) has not been widely explored although the ECM plays a very important role in bone development. In our research we focused on examining the bone matrix of very young animals due to the intense growth process during the first month of life. Structure images and fluorescence spectra of the bone surface were carried out using confocal fluorescence microscope Eclipse Ti-S inverted CLSM (NIKON, Japan) for compact tibia of healthy 7-, 14- and 28-day-old rat newborns after prenatal zidovudine administration in comparison with control. Spectral features of ECM autofluorescence were analyzed statistically by taking into consideration p < 0.05. The CLSM technique allows for simultaneous examination of the structure and autofluorescence from selected areas of the bone surface. Excessive autofluorescence of ECM after prenatal zidovudine administration influences bone growth incommensurably to the newborns’ age. Therefore the possibility of an additional non-enzymatic mechanism of collagen cross-linking in the first two weeks of life of newborn rats prenatally treated with zidovudine has been considered. Our results suggest that ECM autofluorescence can be an indicator of bone development in the normal and pathological state.

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Evaluation of lipofuscin-like pigments as an index of lead-induced oxidative damage in the brain

Cited by 18 publications

References 27 publications

Three different active aldehydes induce the production of advanced lipoxidation end products upon incubation with bovine serum albumin

Three different active aldehydes induce the production of advanced lipoxidation end products upon incubation with bovine serum albumin

HPLC Separation of Fluorescent Products of Lipid Peroxidation in Erythrocytes and Mitochondria

Prenatal Zidovudine Treatment Modifies Early Development of Rat Osteoid – Confocal Microspectroscopy Analysis

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