Evaluation of lipid removal from animal fats for the determination of organochlorine, pesticides, and polychlorinated biphenyl indicators by gas chromatography with electron capture detector

Abstract

For cleanup of animal fat before GC analysis of organochlorine pesticides and polychlorinated biphenyls, freezing-lipid filtration with solid-phase extraction and matrix solid-phase dispersion were evaluated to replace the official Brazilian methodology that uses preparative alumina column chromatography. General drawbacks associated with this last technique, such as the use of large amounts of solvent, laborious and time-consuming procedure could be avoided by using these alternative approaches. Experiments were carried out to study the performance by using different combinations of sorbents and elution solvents. Efficiency of alternative extraction methods in terms of fat removal and recovery capability was monitored by gravimetry, TLC, and GC with electron capture detection. Freezing-lipid filtration with solid-phase extraction afforded better clean up efficiency with recoveries in a range of 54.5 to 103.6% with the relative standard deviation of less than 10% for all compounds under investigation.

Multi‐walled carbon nanotube modified dummy‐template magnetic molecularly imprinted microspheres as solid‐phase extraction material for the determination of polychlorinated biphenyls in fish

“…Polychlorinated biphenyls (PCBs) are toxic and resistant to degradation. They are persistent in the environment and can accumulate up to elevated concentrations in the aquatic organisms . Since PCBs can cause great harm to human beings through the bioaccumulation of the food chain, it could be of great importance to be able to detect PCBs at ultra‐trace levels.…”

Establishment of analysis method for the quantification of residues of halquinol and its metabolites in livestock and fishery products using liquid chromatography–tandem mass spectrometry

“…In this study, we established the halquinol extraction method based on a multi-veterinary drug residue determination method [22], with the following modifications: a cool-down step was added before the centrifugation step of the existing extraction method; for the pork fat sample extraction method in particular, centrifugation was conducted twice after the cool-down step. By adding these steps, we expected to increase the coagulation and precipitation of the fat in the livestock and fishery product samples, which results in a higher efficiency of fat elimination [23]. Also, we added to EDTA in extraction solution for improving the extraction efficiency [16].…”