Evaluation of Light Scattering and Autofluorescent Properties of Brewer's Worts for Flow Cytometric Analysis of Yeast Viability

Boyd, Andrew Robert Burke; Attfield, Paul V.; Veal, Duncan; Vincent, Scott F

doi:10.1002/j.2050-0416.2000.tb00072.x

Cited by 11 publications

(10 citation statements)

References 14 publications

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“…Therefore, the viability of a statistically significant number of cells can be determined rapidly. We have shown previously that flow cytometry (FCM) can be used to determine the viability of oxonol‐stained brewer's yeast samples, without interference from wort particles [11].…”

Section: Introductionmentioning

confidence: 99%

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A flow-cytometric method for determination of yeast viability and cell number in a brewery

Boyd

Gunasekera

Attfield

et al. 2003

FEMS Yeast Research

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A flow-cytometric assay, using the fluorescent dye, oxonol, for the simultaneous determination of yeast cell viability and cell number is described. The assay was optimised, and trialed at a brewery for 6 months. The flow-cytometry assay offered a substantially reduced error in viability determination, compared to methylene blue which is the industry standard for measuring viability. Further, by calculating yeast cell number at the same time, this assay provides a reliable method for determining pitching rate, allowing increased quality control of subsequent fermentations.

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Section: Introductionmentioning

confidence: 99%

“…As the cytometer analyses individual cells, cell size is irrelevant. Further, wort particles are ignored by the software [11].…”

Section: Introductionmentioning

confidence: 99%

A flow-cytometric method for determination of yeast viability and cell number in a brewery

Boyd

Gunasekera

Attfield

et al. 2003

FEMS Yeast Research

View full text Add to dashboard Cite

show abstract

“…The majority of FC applications in this area are related to beer production, being a useful instrument for fermentation optimization and quality control analysis [227,[231][232][233][234][235].…”

Section: Saccharomyces Cerevisiaementioning

confidence: 99%

Application of flow cytometry to industrial microbial bioprocesses

Dı́az

Herrero

Garcı́a

et al. 2010

Biochemical Engineering Journal

243

186

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“…[10], ukazující na fyziologický stav populace, a hodnoty viability, stanovené barvením propidium jodidem s vyhodnocením na průtoko-vém cytometru [11].…”

Section: Materiál a Metodyunclassified

Wort sugars uptake rate and cell cycle of brewing yeast. II. Cell cycle of three brewing yeast strains during pilot plant fermentation.

Novák¹,

Basařová²,

Fiala³

2004

Kvasny Prum

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V předložené práci byl sledován buněčný cyklus tří kmenů pivovarských kvasinek a počet buněk ve vznosu během modelové fermentace. Šlo o kmeny č. 2, č. 7 a č. 95 označené podle sbírky VÚPS. Kvasnice byly odebrány v provozech tří velkých pivovarů, a to po druhém provozním nasazení. Jako růstové médium byla použita mladina z provozu jednoho českého pivovaru. Původní koncentrace extraktu mladiny byla 12 % a část sladu v sypání byla nahrazena nesladovaným ječmenem. Kvašení probíhalo při 13 °C. Nejrychlejší vzestup počtu buněk ve vznosu byl pozorován u kmene č. 7, za kterým násle-doval kmen č. 2 a kmen č. 95. Rychlost nárůstu počtu buněk ve vznosu korelovala u sledovaných kmenů s rychlostí zkvašování sacharidů mladiny, tedy metabolickou aktivitou. Sledováním procentuálního zastoupení buněk v jednotlivých fázích buněčného cyklu byla u všech kmenů určena maxima buněk v G2 a M-fázi buněčného cyklu. Během prvních desítek hodin kvašení došlo k výraznému nárůstu procenta buněk v G2 a M-fázi buněčného cyklu se zpožděním pozorovaným u kmene č. 2. Dále bylo zjištěno, že se sledované kmeny liší i z hlediska rychlosti dosažení maxima buněk v G2 a M-fázi buněčného cyklu, stejně jako v hodnotě tohoto maxima. Prudký pokles buněk v G2 a M-fázi resp. prudký nárůst buněk v G1-fázi se překrýval s obdobím maximální rychlosti zkvašování maltosy. U kmene č. 95 byl ná-růst buněk v G1-fázi nejpomalejší, což se projevilo v rychlosti zkvašování maltosy. The objective of this work was to study the cell cycle of three strains of lager yeast in relation to the number of elevated cells in wort and utilization of main wort sugars.Yeast strains No. 2, 7 and 95 (marked in accordance with RIBM 655 -Culture Collection of Brewing Yeasts) were used in the study. The experiments were carried out in pilot plant scale fermenter at the temperature of 13 °C. The original gravity of wort was 12 % and the malt in grist was partly substituted by unmalted barley. During pilot plant fermentation were observed differences between the studied strains in rise number of elevated cells in wort. The strain No. 7 showed the shortest and fastest phase rise number of elevated cells in wort, next was strain No. 2. The strain No. 95 showed the slowest and longest phase rise number of elevated cells in wort. These results were in correlation with wort sugar uptake rate observed for tested strains. Cell cycle was monitored by propidium iodide staining with evaluation by flow cytometry. During pilot plant fermentations were determinate maxim percentage substitution in population G2/M phase of cell cycle. Expressive growth of percentage substitution G2/M cells was observed during first tens hours of fermentation for all tested strains. The fastest maximum G2/M cells in population was observed for strain No. 7, next were strains No. 2 and 95, this was in relation to process number of elevated cells in wort. Decrease of G2/M phase cells in population was in accordance with maximum extract uptake rate (utilisation of maltose). The slowest decrease of G2/M phase cells in population...

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Evaluation of Light Scattering and Autofluorescent Properties of Brewer's Worts for Flow Cytometric Analysis of Yeast Viability

Cited by 11 publications

References 14 publications

A flow-cytometric method for determination of yeast viability and cell number in a brewery

A flow-cytometric method for determination of yeast viability and cell number in a brewery

Application of flow cytometry to industrial microbial bioprocesses

Wort sugars uptake rate and cell cycle of brewing yeast. II. Cell cycle of three brewing yeast strains during pilot plant fermentation.

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