Evaluation of led light on transgenic Nicotiana benthamiana production of a recombinant antibody fragment scFvanti-BAP1.

Abstract

Plants are an important alternative to produce monoclonal antibodies (mAbs). Although, the plant-based platform still faces several hurdles, that include the choice of plant species to be transformed, the development of suitable plant culture conditions, the genetic lineage of the plant, and the prevention of gene silencing. All of these challenges hinder the enhancement of recombinant productivity. Considering all steps in mAb production, its costs per protein gram are equivalent to that obtained using Chinese hamster ovary cells (CHO cells). Therefore, the construction of a system that optimizes some of these parameters would be welcome. Thus, the development of systems that increase protein production in plant cells are welcome. In the present work, we evaluated three different lineages of a transgenic plant producing mAb scFv-anti-BAP1 under different light conditions. Morphological characteristics, gDNA level and quantitative gene expression were monitored to evaluate the light effects in recombinant protein production. We found that White/Deep Red light increases the total soluble protein production in plants, as well as recombinant yield.