2023
DOI: 10.1590/s1678-9946202365001
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Evaluation of larval surface antigens from infective larvae of Strongyloides venezuelensis for the serodiagnosis of human strongyloidiasis

Abstract: Serodiagnosis of strongyloidiasis is usually performed by ELISA for the detection of IgG antibodies due to its high sensitivity and practicality, but its main limitation is a constant source of S. stercoralis antigens. The use of S. venezuelensis as a heterologous source of antigens has facilitated several published studies on the serodiagnosis and epidemiology of human strongyloidiasis. The main objective of this study was to evaluate the diagnostic accuracy of su… Show more

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Cited by 2 publications
(4 citation statements)
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“…The test has been based on the heterologous extract of S . venezuelensis (CTABL3), with cut-off, sensitivity, and specificity values of 0.286, 95% and 97.8%, respectively, as already established (Gomes et al 2022) [ 33 ]. Positive serum samples for S .…”
Section: Methodsmentioning
confidence: 78%
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“…The test has been based on the heterologous extract of S . venezuelensis (CTABL3), with cut-off, sensitivity, and specificity values of 0.286, 95% and 97.8%, respectively, as already established (Gomes et al 2022) [ 33 ]. Positive serum samples for S .…”
Section: Methodsmentioning
confidence: 78%
“…ELISA for detection of IgG antibodies was based on an established protocol used for research and daily routine use at the Clinical Hospital, School of Medicine, University of São Paulo [ 33 ]. Briefly, commercial 96-well flat bottom microtitration polystyrene plates (Costar, Sigma-Aldrich Chemical Co., St. Louis, MO, USA) were coated (100 μL/well) with the antigenic extracts at a concentration of 10 μg/mL, diluted in carbonate-bicarbonate buffer (0.06 M, pH 9.6), and incubated overnight at 4°C.…”
Section: Methodsmentioning
confidence: 99%
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