The association of age, stature, and trellis height (TH) with the upper body musculoskeletal symptoms (MSS) in Japanese vineyard farmers was investigated to provide basic information for MSS health improvement and the creation of new version of mechanical wearable supports, such as the agri-robot suits. A total of 151 vineyard farmers responded to the questionnaire-based survey about workload and MSS with age, stature, and TH. Logistic regression, linear regression, and correlation analysis was used to test the association of MSS with age, stature, and TH in shoulder, upper back, and low back regions. Different significant risk factors for the upper back, shoulder, and lower back were noted. Older age, high TH, and small height difference (HD) between the stature and TH significantly increased the risk of shoulder, upper back, and lower back MSS, respectively. MSS were higher in the upper body than the lower body. These risk factors vary depending on the specific body region.High incidence rate (100%) was reported in the study population. Among farmers who desired to buy the robot suit (58%), asked for support of the whole body (42%), upper body (46%), and lower body (12%). The risks of MSS in vineyard farmers are high. Intervention measures such as relaxing postural stress by taking a regular break every hour might be beneficial. Lightening the muscle strain of the high risk body parts by reducing considerable exertion with mechanical support is also a great option.The wearable agri-robot suit should be improved, mainly focusing on the specific risk factors of the shoulder, upper back, and lower back regions to reduce physical strain. It is also worth to develop the suit in detachable design which can support whole body, upper part only, and lower part only.