Evaluation of L-index interference limits on Roche cobas c502 and c702 immunoturbidimetric assays using endogenously lipemic specimens and intralipid spiking

Hunsaker, Joshua J.H.; Wyness, Sara P.; Needham, Laura; Genzen, Jonathan R.

doi:10.1016/j.clinbiochem.2019.05.014

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…While clinical devices such as the Afinion Lipid Panel (Abbott) might be best suited for a broad assessment of lipoproteins, their limited availability to all researchers poses problems. There is nonetheless utility in using both quantitative (i.e., flow cytometry, ELISA) and semi‐quantitative methods such as spectrophotometry (turbidimetry) (Hunsaker et al., 2019) or Western blot for these purposes as they can complement downstream assessment of EV preparations (Karimi et al., 2018). Investigators should indicate the method used to detect lipoproteins (item 3.19).…”

Section: Resultsmentioning

confidence: 99%

“…Investigators should indicate the method used to detect lipoproteins (item 3.19). For spectrophotometry, Lindex is commonly used to report lipidaemia, therefore, L-index (item 3.20) and instrument type (item 3.21) should be indicated (Hunsaker et al, 2019). Given MISEV2018 recommends the analysis of at least one marker of apolipoproteins among ApoA1/2, ApoB-100/B-48 to assess lipoprotein abundance in EV samples, it may be appropriate to assess this in the blood derivatives as a means of determining percent reductions (Théry et al, 2018).…”

Section: Platelets ( -)mentioning

confidence: 99%

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MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research

Lucien,

Gustafson,

Lenassi

et al. 2023

J of Extracellular Vesicle

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Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma and serum) are not only donor‐dependent but also influenced by collection and preparation protocols. Since there are hundreds of pre‐analytical protocols and over forty variables, the development of standard operating procedures for EV research is very challenging. To improve the reproducibility of blood EV research, the International Society for Extracellular Vesicles (ISEV) Blood EV Task Force proposes standardized reporting of (i) the applied blood collection and preparation protocol and (ii) the quality of the prepared plasma and serum samples. Gathering detailed information will provide insight into the performance of the protocols and more effectively identify potential confounders in the prepared plasma and serum samples. To collect this information, the ISEV Blood EV Task Force created the Minimal Information for Blood EV research (MIBlood‐EV), a tool to record and report information about pre‐analytical protocols used for plasma and serum preparation as well as assays used to assess the quality of these preparations. This tool does not require modifications of established local pre‐analytical protocols and can be easily implemented to enhance existing databases thereby enabling evidence‐based optimization of pre‐analytical protocols through meta‐analysis. Taken together, insight into the quality of prepared plasma and serum samples will (i) improve the quality of biobanks for EV research, (ii) guide the exchange of plasma and serum samples between biobanks and laboratories, (iii) facilitate inter‐laboratory comparative EV studies, and (iv) improve the peer review process.

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Section: Resultsmentioning

confidence: 99%

Section: Platelets ( -)mentioning

confidence: 99%

MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research

Lucien,

Gustafson,

Lenassi

et al. 2023

J of Extracellular Vesicle

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“…Combinations of lipids and apoB levels are used in the diagnosis of familial chylomicronaemia syndrome (FCS) (ie, TG >10 mmol/L; TG/cholesterol ratio >2.2 (in mmoL/L) and apoB <1 g/L) and to identify patients likely to have apoE variants 39–41. Automated analysers also detect CM particles through the lipaemia index42 and ideally all specimens with a high lipaemic index should have TG measured. Elevated indices indicate TG interference with some assays e.g.…”

Section: Triglyceride-related Laboratory Techniquesmentioning

confidence: 99%

“…Elevated indices indicate TG interference with some assays e.g. plasma sodium or liver transaminases 42…”

Section: Triglyceride-related Laboratory Techniquesmentioning

confidence: 99%

Hypertriglyceridaemia: an update

et al. 2022

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Triglycerides (TGs) form part of the standard lipid profile. Elevations in TGs are associated with increased cardiovascular disease risk through triglyceride-rich lipoprotein particles found as part of non-HDL cholesterol. Many elevations of TGs are secondary to other causes, but primary hypertriglyceridaemia syndromes need to be identified. The genetic causes of hypertriglyceridaemia range from familial combined hyperlipidaemia through the autosomal recessive remnant hyperlipidaemia (related to apolipoprotein E variants) and familial chylomicronaemia syndromes. Patients with primary hypertriglyceridaemia >10 mmol/L require characterisation and specific intervention. Simple lipid profiles do not provide adequate information for detailed diagnosis and additional assays such as apolipoprotein (apo)B100, apoE genotype and next-generation sequencing may be useful. Management of raised TGs includes optimising diet, reducing exacerbating factors as well as lipid-lowering medications such as statins, fibrates, niacin and omega-3 fatty acids. Novel medications for orphan disease indications such as familial chylomicronaemia syndrome include volanesorsen, evinacumab and other antisense therapeutics. Extreme hypertriglyceridaemia syndromes, especially chylomicronaemia syndromes, which can be exposed by pregnancy or other factors are a medical emergency and require admission and specialist management sometimes including plasma exchange.

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“…Most of the interference studies have been done with IVLE and lipemia interference in commercial products is also based on IV-LE-spiked studies. Studies with native lipemic samples are limited (12,(14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Comparison of lipemia interference created with native lipemic material and intravenous lipid emulsion in emergency laboratory tests

Şeneş,

Bolat,

Sürer

et al. 2024

Biochem. med. (Online)

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Evaluation of L-index interference limits on Roche cobas c502 and c702 immunoturbidimetric assays using endogenously lipemic specimens and intralipid spiking

Cited by 7 publications

References 22 publications

MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research

MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research

Hypertriglyceridaemia: an update

Comparison of lipemia interference created with native lipemic material and intravenous lipid emulsion in emergency laboratory tests

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