Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry

Plikusienė, Ieva; Mačiulis, Vincentas; Ramanavičienė, Almira; Balevičius, Zigmas; Bužavaitė-Vertelienė, Ernesta; Čiplys, Evaldas; Slibinskas, Rimantas; Simanavičius, Martynas; Žvirblienė, Aurelija

doi:10.1016/j.jcis.2021.02.100

Cited by 41 publications

(41 citation statements)

References 34 publications

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“…This method can be used for the efficient diagnosis of SARS coronavirus. Recently, Plikusiene et al [ 40 ] published a comprehensive article and they used a total internal reflection ellipsometry method for detection of SARS-CoV-2. Although in this article, they used a Au bulk disc for determination and its methodology is behind of the present study; but they have an interesting discussion on kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies immobilized on Au disc.…”

Section: Optical Methodsmentioning

confidence: 99%

A Minireview on Nanoparticle-Based Sensors for the Detection of Coronaviruses

2021

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Coronaviruses (CoVs) are a class of viruses that cause respiratory tract infections in birds and mammals. Severe acute respiratory syndrome and Middle East respiratory syndrome are pathogenic human viruses. The ongoing coronavirus causing a pandemic of COVID-19 is a recently identified virus from this group. The first step in the control of spreading the disease is to detect and quarantine infected subjects. Consequently, the introduction of rapid and reliable detection methods for CoVs is crucial. To date, several methods were reported for the detection of coronaviruses. Nanoparticles play an important role in detection tools, thanks to their high surface-to-volume ratio and exclusive optical property enables the development of susceptible analytical nanoparticle-based sensors. The studies performed on using nanoparticles-based (mainly gold) sensors to detect CoVs in two categories of optical and electrochemical were reviewed here. Details of each reported sensor and its relevant analytical parameters are carefully provided and explained.

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Section: Optical Methodsmentioning

confidence: 99%

A Minireview on Nanoparticle-Based Sensors for the Detection of Coronaviruses

2021

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“…Recently, spectroscopic ellipsometry (SE) in total internal reflection mode (TIRE) was applied for the monitoring the kinetic of interactions between on SAM-modified gold disk immobilized SARS-CoV-2 N-protein and antibodies against it [122]. TIRE allowed detecting biomolecules mass changes at solid-liquid interface by phase shift measurement.…”

Section: Ellipsometry and Spr Based Immunosensorsmentioning

confidence: 99%

“…The high sensitivity of SE TIRE was attained with the support of SPR, what enabled the registration of two kinetic curves Ψ(t) and ∆(t) simultaneously [123,124]. It was reported, that antigenantibody complex is strongly bound and the complex formation has very strict orientation requirements, which was established by meaning of mathematical model building [122]. The main working element of the sensor is the piezoelectric resonator, on which an antigen or antibody is immobilized using SAM-based technology.…”

Section: Ellipsometry and Spr Based Immunosensorsmentioning

confidence: 99%

Affinity Sensors for the Diagnosis of COVID-19

et al. 2021

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The coronavirus disease 2019 (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was proclaimed a global pandemic in March 2020. Reducing the dissemination rate, in particular by tracking the infected people and their contacts, is the main instrument against infection spreading. Therefore, the creation and implementation of fast, reliable and responsive methods suitable for the diagnosis of COVID-19 are required. These needs can be fulfilled using affinity sensors, which differ in applied detection methods and markers that are generating analytical signals. Recently, nucleic acid hybridization, antigen-antibody interaction, and change of reactive oxygen species (ROS) level are mostly used for the generation of analytical signals, which can be accurately measured by electrochemical, optical, surface plasmon resonance, field-effect transistors, and some other methods and transducers. Electrochemical biosensors are the most consistent with the general trend towards, acceleration, and simplification of the bioanalytical process. These biosensors mostly are based on the determination of antigen-antibody interaction and are robust, sensitive, accurate, and sometimes enable label-free detection of an analyte. Along with the specification of biosensors, we also provide a brief overview of generally used testing techniques, and the description of the structure, life cycle and immune host response to SARS-CoV-2, and some deeper details of analytical signal detection principles.

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“…The irst technique is targeted to the N-gene and E-gene of SARS-CoV-2 and engages the Cas12a enzyme, on the other hand, the second one is targeted at the N gene with the utilization of Cas12b enzyme. PCR-based tests take center stage to maintain the standard of diagnostic tests [76,77]. However, a labor-intensive protocol with long waiting for results, and a dwindling reagents supply has led to many scientists looking for alternative CRISPR test.…”

Section: Crispr-based Diagnosticsmentioning

confidence: 99%

Untitled

2021

Int J Clin Virol

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The Coronavirus disease-2019 (COVID-19), has become a worldwide pandemic and the scientifi c communities are struggling to fi nd out the ultimate treatment strategies against this lethal virus, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Presently, there is no potential chemically proven antiviral therapy available in the market which can eff ectively combat the infection caused by this deadly virus. Few vaccines are already developed but it is not clear to the scientifi c community how much effi cient they are to combat SARS-CoV-2. Mode of transmission and symptoms of the disease are two important factors in this regard. Rapid diagnosis of the COVID-19 is very much important to stop its spreading. In this scenario, a complete study starting from symptoms of the disease to vaccine development including various SARS-CoV-2 detection techniques is very much required. In this review article, we have made a partial analysis on the origin, virology, global health, detection techniques, replication pathways, doses, mode of actions of probable drugs, and vaccine development for SARS-CoV-2.

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Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry

Abstract: Graphical abstract

Cited by 41 publications

References 34 publications

A Minireview on Nanoparticle-Based Sensors for the Detection of Coronaviruses

A Minireview on Nanoparticle-Based Sensors for the Detection of Coronaviruses

Affinity Sensors for the Diagnosis of COVID-19

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