Evaluation of KI-67 expression in uterine leiomyoma and in healthy myometrium: a pilot study

Filho, Walberto Monteiro Neiva Eulálio; Soares, Eduardo Augusto Sousa; Lima, Maria Simone Oliveira; Brazil, Emerson Davi do Nascimento; Rodrigues, Rodolfo Myronn de Melo; Zeron, Rubens Moura Campos; Silva, Benedito Borges; Costa, Pedro Vítor Lopes

doi:10.1590/1806-9282.65.12.1459

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…However, already on Day 3, the proliferation marker is downregulated to more physiological rates found in healthy tissues. For example, in healthy myometrium, only 1.6% of all cells were found to be Ki67 positive (Eulálio Filho et al, 2019). In conclusion, spheroids are more suitable for reflecting the situation of cells in tissues better than 2D‐cultures.…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric quantification of apoptotic and proliferating cells applying an improved method for dissociation of spheroids

Metzger

Rösch

Sossong

et al. 2021

Cell Biology International

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Spheroids are a promising tool for many cell culture applications, but their microscopic analysis is limited. Flow cytometry on a single cell basis, which requires a gentle but also efficient dissociation of spheroids, could be an alternative analysis.Mono-culture and coculture spheroids consisting of human fibroblasts and human endothelial cells were generated by the liquid overlay technique and were dissociated using AccuMax as a dissociation agent combined with gentle mechanical forces. This study aimed to quantify the number of apoptotic and proliferative cells.We were able to dissociate spheroids of differing size, age, and cellular composition in a single-step dissociation protocol within 10 min. The number of single cells was higher than 95% and in most cases, the viability of the cells after dissociation was higher than 85%. Coculture spheroids exhibited a higher sensitivity as shown by lower viability, higher amount of cellular debris, and a higher amount of apoptotic cells. Considerable expression of the proliferation marker Ki67 could only be seen in 1-day-old spheroids but was already downregulated on Day 3. In summary, our dissociation protocol enabled a fast and gentle dissociation of spheroids for the subsequent flow cytometric analysis. The chosen cell type had a strong influence on cell viability and apoptosis. Initially high rates of proliferative cells decreased rapidly and reached values of healthy tissue 3 days after generation of the spheroids. In conclusion, the flow cytometry of dissociated spheroids could be a promising analytical tool, which could be ideally combined with microscopic techniques.

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Section: Discussionmentioning

confidence: 99%

Flow cytometric quantification of apoptotic and proliferating cells applying an improved method for dissociation of spheroids

Metzger

Rösch

Sossong

et al. 2021

Cell Biology International

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“…Surgery can trigger the development of new myomatous nodes, because damage to the myometrium activates the expression of mitogenic and angiogenic growth factors. High level of Ki-67 (Ki-67), progesterone receptor (PgR) and vascular endothelial growth factor (VEGF) in the myometrium and fibroids are pathogenetic factors of UF recurrence 22 – 24 .…”

Section: Introductionmentioning

confidence: 99%

Alterations in lipid profile upon uterine fibroids and its recurrence

Tonoyan

Chagovets

Стародубцева

et al. 2021

Sci Rep

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Uterine fibroids (UF) is the most common (about 70% cases) type of gynecological disease, with the recurrence rate varying from 11 to 40%. Because UF has no distinct symptomatology and is often asymptomatic, the specific and sensitive diagnosis of UF as well as the assessment for the probability of UF recurrence pose considerable challenge. The aim of this study was to characterize alterations in the lipid profile of tissues associated with the first-time diagnosed UF and recurrent uterine fibroids (RUF) and to explore the potential of mass spectrometry (MS) lipidomics analysis of blood plasma samples for the sensitive and specific determination of UF and RUF with low invasiveness of analysis. MS analysis of lipid levels in the myometrium tissues, fibroids tissues and blood plasma samples was carried out on 66 patients, including 35 patients with first-time diagnosed UF and 31 patients with RUF. The control group consisted of 15 patients who underwent surgical treatment for the intrauterine septum. Fibroids and myometrium tissue samples were analyzed using direct MS approach. Blood plasma samples were analyzed using high performance liquid chromatography hyphened with mass spectrometry (HPLC/MS). MS data were processed by discriminant analysis with projection into latent structures (OPLS-DA). Significant differences were found between the first-time UF, RUF and control group in the levels of lipids involved in the metabolism of glycerophospholipids, sphingolipids, lipids with an ether bond, triglycerides and fatty acids. Significant differences between the control group and the groups with UF and RUF were found in the blood plasma levels of cholesterol esters, triacylglycerols, (lyso) phosphatidylcholines and sphingomyelins. Significant differences between the UF and RUF groups were found in the blood plasma levels of cholesterol esters, phosphotidylcholines, sphingomyelins and triacylglycerols. Diagnostic models based on the selected differential lipids using logistic regression showed sensitivity and specificity of 88% and 86% for the diagnosis of first-time UF and 95% and 79% for RUF, accordingly. This study confirms the involvement of lipids in the pathogenesis of uterine fibroids. A diagnostically significant panel of differential lipid species has been identified for the diagnosis of UF and RUF by low-invasive blood plasma analysis. The developed diagnostic models demonstrated high potential for clinical use and further research in this direction.

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Predicting recurrence of uterine myoma after carrying out a selective uterine arteries embolization

et al. 2022

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Aim. To establish clinic and anamnestic predictors for recurrence of uterine myomas growth after carrying out a selective uterine arteries embolization (s-UAE). Materials and methods. The one-center longitudinal retrospective cohort study including 366 women with symptomatic uterine myoma, after carrying out s-UAE at 20132017. The Control group 315 patients after s-UAE with absolute reduction of blood-circulation and strong remission for 3 years. The main group 51 cases of restoration of clinical symptoms and blood-groove after ultrasonography registered absolute reduction and knocking over of clinical symptoms more than two years period. The median of age of control group patients has made 40 years (95% confidence interval 95% CI 39.041.1), the main 42 years (95% CI 39.043.0). In group of comparison it is carried out the analysis of 103 potential risk factors for uterine myomas relapse after s-EMA. For the correlation between uterine myomas relapse and subset quantitative and qualitative signs the model of the logistic regressions was used with a step-by-step elimination of a sign. Results. Statistically significant risk factors for recurrence of uterine myomas after s-EMA are established: body mass index (BMI) 22,3 (odds ratio OR 4.03); previous plentiful menstrual bleedings (OR 3.56); the previous hormonal therapy (OR 3.06); hypertension (OR 2.58); chronic cystitis (OR 2.18); dyspareunia (OR 2.22); the conglomerate including 23 uterine myomas (OR 1.79); an edge localization of uterine myomas (OR 2.24). Use a logit-models, with inclusion of these specific signs allows to predict uterine myomas growth recurrence after s-EMA in 78.8% cases (95% Cl 0.760.83; р0.0001). Conclusion. At preoperative consultation stage to patients with symptomatic uterine myoma establishment a combination of criteria "body mass index" (BMI)22.3, previous plentiful menstrual bleedings and previous hormonal therapy, assumes to refuse carrying out s-EMA in advantage alternative method of treatment.

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Evaluation of KI-67 expression in uterine leiomyoma and in healthy myometrium: a pilot study

Cited by 3 publications

References 7 publications

Flow cytometric quantification of apoptotic and proliferating cells applying an improved method for dissociation of spheroids

Flow cytometric quantification of apoptotic and proliferating cells applying an improved method for dissociation of spheroids

Alterations in lipid profile upon uterine fibroids and its recurrence

Predicting recurrence of uterine myoma after carrying out a selective uterine arteries embolization

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