Evaluation of Japakusumadi Yoga for safety profile - Acute Toxicity Profile

Abstract

Japakusumadi Yoga is an Ayurvedic formulation, indicated for contraception in Ayurveda. This study determines acute toxicity of ‘Japakusumadi Yoga’ an oral formulation in wister albino rats. Single dose acute toxicity was assessed by employing OECD guidelines 425 using AOT software. Test formulations was administered to overnight fasted animals and 14 days observation of dosed (up down as per requirement) rats was done for general appearance, cage side behaviour including increased or decreasing motor activity, convulsions, straub’s reaction, catatonia, muscle spasm, spasticity, ophisthotonus, hyperesthesia, muscle relaxation, anaesthesia, arching and rolling, lacrimation, salivation, diarrhoea, writhing movement, mode of respiration and changes in skin colour etc., with mortality and autopsy finding in case of dead animal. Based on the observation made and recorded it can be concluded that the test drug is without any toxic potential even at the dose of 2000 mg/kg in animals equivalent to 22.4g for human being.