Evaluation of isolated probiotics on the efficacy of immune system in male and female Wistar rats

Bibi, Zuhra; Ashraf, Khadija; Shehzadi, Areeba; Rehman, Abdul; Bukhari, Dilara Abbas

doi:10.1016/j.jsps.2023.04.023

Cited by 3 publications

(1 citation statement)

References 49 publications

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“…Probiotics to be used were available in Cell and Molecular Biology Laboratory that were previously isolated from dairy and non-dairy products ( Bibi et al 2023 ). These strains included Lactiplantibacillus plantarum MZ707748 , L. plantarum MZ710117 , L. plantarum MZ735961 , and Weisella confusa MZ727611 .…”

Section: Methodsmentioning

confidence: 99%

Effect of probiotics on hematological parameters of male and female Wistar rats

Shehzadi,

Bibi,

Qadeer Sarwar

et al. 2023

Saudi Journal of Biological Sciences

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Section: Methodsmentioning

confidence: 99%

Effect of probiotics on hematological parameters of male and female Wistar rats

Shehzadi,

Bibi,

Qadeer Sarwar

et al. 2023

Saudi Journal of Biological Sciences

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Long-Term Treatment with Alcaligenes faecalis A12C Improves Host Resistance to Pathogens in Septic Rats: Possible Contribution of Curdlan-Like Immune Trainer

Martel-Benítez,

Alayón-Afonso,

Castillo

et al. 2024

Probiotics & Antimicro. Prot.

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Curdlan, a β-1,3/1,6-glucan found in Alcaligenes faecalis (A. faecalis) wall, activates innate and humoral immunity. The aim of this study is to evaluate whether pretreated rats with A. faecalis A12C could prevent sepsis disturbances and identify the immunomodulatory mechanisms involved. Experiments occurred in two stages: a survival study with 16 rats randomly divided into septic (SC) (n = 8) and septic pretreated (SA) (n = 8) groups and 45 rats divided into four groups: healthy (AGUSAN) (n = 9), septic (AGUIC) (n = 13), septic pretreated (AGUIA) (n = 14), and healthy pretreated (AGUSTO) (n = 9). Sepsis was induced by cecal ligation and puncture after 30 days of A. faecalis A12C pretreatment or without. SA group had a higher survival rate of 58% vs. 16% for SC group (P < 0.05). Overall, AGUIA showed better status than AGUIC (P < 0.01). Higher monocytosis was found in AGUIA and AGUSTO vs. AGUIC and AGUSAN, respectively (P < 0.05). A gradual increase in curdlan fecal concentration was observed in AGUIA during pretreatment. Fecal concentrations of Escherichia coli significantly decreased in AGUIA and AGUSTO. Bacterial load in urine, peritoneal lavage fluid (PLF), and bronchoalveolar lavage fluid (BALF) decreased (P < 0.05) in AGUIA vs. AGUIC. Finally, lower inflammation was observed in serum, BALF, and PLF, with reduced IL-6, IL-10, IL-1β, and TNF-α, along with less damage in lungs and peritoneum in AGUIA vs. AGUIC. These findings suggest the connection between curdlan—produced by A. faecalis A12C—with the immune system and the reduction in severity of experimental sepsis.

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Long-term treatment with Alcaligenes faecalis A12C improves host resistance to pathogens in septic rats: possible contribution of curdlan-like immune-trainer

Martel-Benítez,

Alayón-Afonso,

Castillo

et al. 2023

Preprint

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Curdlan, a β-1,3/1,6-glucan found in Alcaligenes faecalis (A. faecalis) wall, activates innate and humoral immunity. The aim of this study is to evaluate whether pre-treated rats with A.faecalis A12C could prevent sepsis disturbances and identify the immunomodulatory mechanisms involved. Experiments occurred in two stages: a survival study with 16 rats randomly divided into septic (SC) (n = 8) and septic pre-treated (SA) (n = 8) groups; and 45 rats divided into four groups: healthy (AGUSAN) (n = 9), septic (AGUIC) (n = 13), septic pre-treated (AGUIA) (n = 14), and healthy pre-treated (AGUSTO) (n = 9). Sepsis was induced by cecal ligation and puncture after 30 days of A.faecalis A12C pre-treatment or without. SA group had a higher survival rate (58%) vs SC group (16%) (P < 0.05). Overall, AGUIA showed better status than AGUIC (P < 0.01). Higher monocytosis was found in AGUIA and AGUSTO vs AGUIC and AGUSAN, respectively (P < 0.05). A gradual increase in curdlan fecal concentration was observed in AGUIA during pre-treatment. Fecal concentrations of E. coli significantly decreased in AGUIA and AGUSTO. Bacterial load in urine, peritoneal, and bronchoalveolar lavage fluids (PLF and BALF) decreased (P < 0.05) in AGUIA vs AGUIC. Finally, lower inflammation was observed in serum, BALF, and PLF, with reduced IL-6, IL-10, IL-1β, and TNF-α, along with less damage in lungs and peritoneum in AGUIA vs AGUIC. These findings suggest the connection between curdlan -produced by A. faecalis A12C- with the immune system and the reduction in severity of experimental sepsis.

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Evaluation of isolated probiotics on the efficacy of immune system in male and female Wistar rats

Cited by 3 publications

References 49 publications

Effect of probiotics on hematological parameters of male and female Wistar rats

Effect of probiotics on hematological parameters of male and female Wistar rats

Long-Term Treatment with Alcaligenes faecalis A12C Improves Host Resistance to Pathogens in Septic Rats: Possible Contribution of Curdlan-Like Immune Trainer

Long-term treatment with Alcaligenes faecalis A12C improves host resistance to pathogens in septic rats: possible contribution of curdlan-like immune-trainer

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