Evaluation of Ionic Liquids and Ionic Liquids Active Pharmaceutical Ingredients Inhibition in Elastase Enzyme Activity

Mota, Fátima A R; Pereira, Sarah A.P.; Araújo, André R. T. S.; Saraiva, M. Lúcia M.F.S.

doi:10.3390/molecules26010200

Cited by 13 publications

(10 citation statements)

References 37 publications

(20 reference statements)

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“…Porcine pancreatic elastase inhibition assay was performed according to Mota and co-workers [ 57 ] with slight modifications. Aqueous extracts were prepared in water, while acetone extracts were prepared in DMSO.…”

Section: Methodsmentioning

confidence: 99%

Cyanobacteria Secondary Metabolites as Biotechnological Ingredients in Natural Anti-Aging Cosmetics: Potential to Overcome Hyperpigmentation, Loss of Skin Density and UV Radiation-Deleterious Effects

et al. 2022

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The loss of density and elasticity, the appearance of wrinkles and hyperpigmentation are among the first noticeable signs of skin aging. Beyond UV radiation and oxidative stress, matrix metalloproteinases (MMPs) assume a preponderant role in the process, since their deregulation results in the degradation of most extracellular matrix components. In this survey, four cyanobacteria strains were explored for their capacity to produce secondary metabolites with biotechnological potential for use in anti-aging formulations. Leptolyngbya boryana LEGE 15486 and Cephalothrix lacustris LEGE 15493 from freshwater ecosystems, and Leptolyngbya cf. ectocarpi LEGE 11479 and Nodosilinea nodulosa LEGE 06104 from marine habitats were sequentially extracted with acetone and water, and extracts were analyzed for their toxicity in cell lines with key roles in the skin context (HaCAT, 3T3L1, and hCMEC). The non-toxic extracts were chemically characterized in terms of proteins, carotenoids, phenols, and chlorophyll a, and their anti-aging potential was explored through their ability to scavenge the physiological free radical superoxide anion radical (O2•−), to reduce the activity of the MMPs elastase and hyaluronidase, to inhibit tyrosinase and thus avoid melanin production, and to block UV-B radiation (sun protection factor, SPF). Leptolyngbya species stood out for anti-aging purposes: L. boryana LEGE 15486 presented a remarkable SPF of 19 (at 200 µg/mL), being among the best species regarding O2•− scavenging, (IC50 = 99.50 µg/mL) and also being able to inhibit tyrosinase (IC25 = 784 µg/mL), proving to be promising against UV-induced skin-aging; L. ectocarpi LEGE 11479 was more efficient in inhibiting MMPs (hyaluronidase, IC50 = 863 µg/mL; elastase, IC50 = 391 µg/mL), thus being the choice to retard dermal density loss. Principal component analysis (PCA) of the data allowed the grouping of extracts into three groups, according to their chemical composition; the correlation of carotenoids and chlorophyll a with MMPs activity (p < 0.01), O2•− scavenging with phenolic compounds (p < 0.01), and phycocyanin and allophycocyanin with SPF, pointing to these compounds in particular as responsible for UV-B blockage. This original survey explores, for the first time, the biotechnological potential of these cyanobacteria strains in the field of skin aging, demonstrating the promising, innovative, and multifactorial nature of these microorganisms.

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Section: Methodsmentioning

confidence: 99%

Cyanobacteria Secondary Metabolites as Biotechnological Ingredients in Natural Anti-Aging Cosmetics: Potential to Overcome Hyperpigmentation, Loss of Skin Density and UV Radiation-Deleterious Effects

et al. 2022

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“…Enzyme inhibition assay is considered a good predictor of overall ILs toxicity, and it is widely used for the assessment of the environmental safety of ionic liquids. The methods most commonly referred to are based on acetylcholinesterase (AChE) [ 42 ] and DNA-polymerase enzymes [ 9 ], but also AMP deaminase [ 43 ], monooxygenase P450 [ 44 ] or elastase [ 45 ] based assays have been used.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Virucidal Potential of Morpholinium-Based Ionic Liquids

Michalski

Sommer

Rossmanith

et al. 2023

IJMS

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Witnessed by the ongoing spread of antimicrobial resistant bacteria as well as the recent global pandemic of the SARS-CoV-2 virus, the development of new disinfection strategies is of great importance, and novel substance classes as effective antimicrobials and virucides are urgently needed. Ionic liquids (ILs), low-melting salts, have been already recognized as efficient antimicrobial agents with prospects for antiviral potential. In this study, we examined the antiviral activity of 12 morpholinium based herbicidal ionic liquids with a tripartite test system, including enzyme inhibition tests, virucidal activity determination against five model viruses and activity against five bacterial species. The antimicrobial and enzymatic tests confirmed that the inhibiting activity of ILs corresponds with the number of long alkyl side chains and that [Dec2Mor]+ based ILs are promising candidates as novel antimicrobials. The virucidal tests showed that ILs antiviral activity depends on the type and structure of the virus, revealing enveloped Phi6 phage as highly susceptible to the ILs action, while the non-enveloped phages PRD1 and MS2 proved completely resistant to ionic liquids. Furthermore, a comparison of results obtained for P100 and P001 phages demonstrated for the first time that the susceptibility of viruses to ionic liquids can be dependent on differences in the phage tail structure.

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“…Elastase inhibition assay was determined as reported by Mota et al [ 34 ]. First, 50 μL of extract dilutions (31.2 to 1000.0 μg mL −1 ) plus 87.5 μL of HEPES buffer (0.1 M with NaCl 0.5 M, pH 7.5), 10 μL of the substrate (N-succinyl-Ala-Ala-Ala p-nitroanilide, 1.12 mg mL −1 ), 70 μL of acetate buffer (200 mM, pH 5.5) and 2.5 μL of DMSO were added to a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

Cosmetic Potential of Pigments Extracts from the Marine Cyanobacterium Cyanobium sp.

Pagels

Almeida²,

Vasconcelos

et al. 2022

Marine Drugs

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The current mindset in the cosmetics market about sustainable ingredients had increased the search for new sources of natural active ingredients. Cyanobacteria are a great source of functional ingredients for cosmetics, as a producer of pigments with described bioactive potential (carotenoids and phycobiliproteins). This work aimed to evaluate the cosmetic potential of marine cyanobacterium Cyanobium sp. pigment-targeted extracts (carotenoids and phycobiliproteins), evaluating their in vitro safety through cytotoxicity assays, cosmetic-related enzyme inhibition, ingredient stability, and putative product (serum formulation). Results showed no cytotoxicity from the extracts in skin-related cell lines. Carotenoid extract showed anti-hyaluronidase capacity (IC50 = 108.74 ± 5.74 mg mL−1) and phycobiliprotein extract showed anti-hyaluronidase and anti-collagenase capacity (IC50 = 67.25 ± 1.18 and 582.82 ± 56.99 mg mL−1, respectively). Regarding ingredient and serum stability, both ingredients showed higher stability at low-temperature conditions, and it was possible to maintain the pigment content and bioactive capacity stable during the tested period, although in higher temperatures the product was degraded in a week. As a major conclusion, both extracts can be potential natural and sustainable ingredients for cosmetic uses, with relatively simple formulation and storage, and can be promising natural anti-aging ingredients due to their bioactive capacity.

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Evaluation of Ionic Liquids and Ionic Liquids Active Pharmaceutical Ingredients Inhibition in Elastase Enzyme Activity

Cited by 13 publications

References 37 publications

Cyanobacteria Secondary Metabolites as Biotechnological Ingredients in Natural Anti-Aging Cosmetics: Potential to Overcome Hyperpigmentation, Loss of Skin Density and UV Radiation-Deleterious Effects

Cyanobacteria Secondary Metabolites as Biotechnological Ingredients in Natural Anti-Aging Cosmetics: Potential to Overcome Hyperpigmentation, Loss of Skin Density and UV Radiation-Deleterious Effects

Antimicrobial and Virucidal Potential of Morpholinium-Based Ionic Liquids

Cosmetic Potential of Pigments Extracts from the Marine Cyanobacterium Cyanobium sp.

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