Evaluation of intra-articular delivery of hyaluronic acid functionalized biopolymeric nanoparticles in healthy rat knees

Zille, Hervé; Paquet, Joseph; Henrionnet, Christel; Scala‐Bertola, Julien; Léonard, Michèle; Six, Jean‐Luc; Deschamp, Frantz; Netter, Patrick; Vergés, J.; Gillet, Pierre; Grossin, Laurent

doi:10.3233/bme-2010-0637

Cited by 30 publications

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“…By presenting IL-1Ra on the particle surface, we provide cells with easy access to the protein and increase the potency of our particle system. Although effective protein delivery from particles is still a challenge, this work adds to promising strategies, such as the phage-panned peptide-targeted nanoparticles [48], hyaluronic acid-coated PLGA particles [49], and chondroitin sulfate-coated gelatin particles [32]. …”

Section: Discussionmentioning

confidence: 99%

Self-assembling nanoparticles for intra-articular delivery of anti-inflammatory proteins

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Self-assembling nanoparticles for intra-articular delivery of anti-inflammatory proteins

et al. 2012

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“…Many types of particulate carriers have been investigated for increasing the retention time of therapeutic agents within the knee cavity, including the following:liposomes (6–11);microparticles of poly(lactide) (PLA), poly-L-lactide acid (PLLA), and poly(lactide-co-glycolide) (PLGA), including hyaluronate-functionalized PLA/PLGA (12–18);albumin microparticles (19,20);chitosan (21);magnetic nanoparticles (22,23);solid lipid nanoparticles (24);thermally responsive elastin-like polypeptides (ELPs) (25);pH-sensitive gels (26); andnanoparticles targeted to the cartilage using phage display peptides (27). …”

Section: Introductionmentioning

confidence: 99%

“…microparticles of poly(lactide) (PLA), poly-L-lactide acid (PLLA), and poly(lactide-co-glycolide) (PLGA), including hyaluronate-functionalized PLA/PLGA (12–18);…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles for Improved Local Retention after Intra-Articular Injection into the Knee Joint

Morgen¹,

Tung²,

Boras

et al. 2012

Pharm Res

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PurposeTo evaluate using cationic polymeric nanoparticles that interact with hyaluronate to form ionically cross-linked hydrogels to increase the intra-articular retention time of osteoarthritis drugs in the synovial cavity.MethodsIn vitro tests included nanoparticle release from cross-linked hydrogels using syringe and membrane dissolution tests, viscosity measurement of synovial fluid containing hydrogels, and release-rate measurement for a model active conjugated to a cationically substituted dextran using a hydrolyzable ester linkage in a sink dissolution test. Nanoparticle retention after intra-articular injection into rat knees was measured in vivo using fluorescence molecular tomography.ResultsDiffusional and convective transport of cationic nanoparticles from ionically cross-linked hydrogels formed in synovial fluid was slower in vitro than for uncharged nanoparticles. Hydrogels formed after the nanoparticles were mixed with synovial fluid did not appreciably alter the viscosity of the synovial fluid in vitro. In vitro release of a conjugated peptide from the cationic nanoparticles was approximately 20% per week. After intra-articular injection in rat knees, 70% of the nanoparticles were retained in the joint for 1 week.ConclusionsThis study demonstrates the feasibility of using cationic polymeric nanoparticles to increase the retention of therapeutic agents in articular joints for indications such as osteoarthritis.

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“…This leads to chemotactic activities, which trigger the complement system cascade and inflammatory cell response to the site of nanoparticle deposition. 19 Zille et al 22 have also reported the slight inflammatory cell response due to the presence of nanoparticles. In this work, poly (D, Llactic-co-glycolic acid) nanoparticles, coated with esterified amphiphilic hyaluronate showed limited hyperplasia as well as slight inflammatory state of the synovial membrane, when delivered one or two times weekly at intra-articular site in vivo.…”

Section: Discussionmentioning

confidence: 96%

Flow Cytometry Analysis of Cytotoxicity In Vitro and Long‐Term Toxicity of HA‐40 wt% BaTiO₃ Nanoparticles In Vivo

2015

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The development of new implantable biomaterials requires bone‐mimicking physical properties together with desired biocompatible property. In continuation to our earlier published research to establish compositional dependent multifunctional bone‐like properties and cytocompatibility response of hydroxyapatite (HA)‐BaTiO3 composites, the toxicological property evaluation, both in vitro and in vivo, were conducted on HA‐40 wt% BaTiO3 and reported in this work. In particular, this work reports in vitro cytotoxicity of mouse myoblast cells as well as in vivo long‐term tissue and nanoparticles interaction of intra‐articularly injected HA‐40 wt% BaTiO3 and BaTiO3 up to the concentration of 25 mg/mL in physiological saline over 12 weeks in mouse model. The careful analysis of flow cytometry results could not reveal any statistically significant difference in terms of early/late apoptotic cells or necrotic cells over 8 d in culture. Extensive histological analysis could not record any signature of cellular level toxicity or pronounced inflammatory response in vital organs as well as at knee joints of Balb/c mice after 12 weeks. Taken together, this study establishes nontoxic nature of HA‐40 wt% BaTiO3 and therefore, HA‐40 wt% BaTiO3 can be used safely for various biomedical applications.

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Evaluation of intra-articular delivery of hyaluronic acid functionalized biopolymeric nanoparticles in healthy rat knees

Cited by 30 publications

References 0 publications

Self-assembling nanoparticles for intra-articular delivery of anti-inflammatory proteins

Self-assembling nanoparticles for intra-articular delivery of anti-inflammatory proteins

Nanoparticles for Improved Local Retention after Intra-Articular Injection into the Knee Joint

Flow Cytometry Analysis of Cytotoxicity In Vitro and Long‐Term Toxicity of HA‐40 wt% BaTiO₃ Nanoparticles In Vivo

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Evaluation of intra-articular delivery of hyaluronic acid functionalized biopolymeric nanoparticles in healthy rat knees

Cited by 30 publications

References 0 publications

Self-assembling nanoparticles for intra-articular delivery of anti-inflammatory proteins

Self-assembling nanoparticles for intra-articular delivery of anti-inflammatory proteins

Nanoparticles for Improved Local Retention after Intra-Articular Injection into the Knee Joint

Flow Cytometry Analysis of Cytotoxicity In Vitro and Long‐Term Toxicity of HA‐40 wt% BaTiO3 Nanoparticles In Vivo

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Product

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Flow Cytometry Analysis of Cytotoxicity In Vitro and Long‐Term Toxicity of HA‐40 wt% BaTiO₃ Nanoparticles In Vivo