Evaluation of intra- and inter-lab variability in quantifying SARS-CoV-2 in a state-wide wastewater monitoring network

Abstract

In December 2019, SARS-CoV-2, the virus that causes coronavirus disease 2019, was first reported and subsequently triggered a global pandemic. Wastewater monitoring, a strategy for quantifying viral gene concentrations from...

Ohio Coronavirus Wastewater Monitoring Network: Implementation of Statewide Monitoring for Protecting Public Health

“…In general, the average N2 gene fragments/L spanned 1.5 log 10 among the laboratories in the network during each trial (range, 0.7-2.2 log 10 ). 22 Like other studies, some network laboratories observed differences between N1 and N2 gene fragment copies/L, while other laboratories did not see differences between these 2 N markers. 12,15,[23][24][25][26][27] In July 2022, the OCWMN transitioned the sample analyses from the multiple research laboratories to ODH Public Health Laboratory (PHL).…”

“…From the point of network initiation until December 2021, 8 split-sample trials were performed. In general, the average N2 gene fragments/L spanned 1.5 log 10 among the laboratories in the network during each trial (range, 0.7-2.2 log 10 ) 22. Like other studies, some network laboratories observed differences between N1 and N2 gene fragment copies/L, while other laboratories did not see differences between these 2 N markers 12,15,23–27…”