Evaluation of Intestinal Permeability of Vicenin-2 and Lychnopholic Acid from Lychnophora salicifolia (Brazilian Arnicão) Using Caco-2 Cells

Abstract

Lychnophora salicifolia, commonly known as "arnicão", is used as an anti-inflammatory agent and as a flavoring agent in the Brazilian traditional spirit "cachaça". In this work, the permeation process of vicenin-2 (1) and lychnopholic acid (2) (major secondary metabolites from the hydroalcoholic extract) was investigated using Caco-2 cells. For this investigation, a new HPLC-DAD method was developed and validated for the quantification step. It was observed that 2 crosses the Caco-2 cell monolayer by passive diffusion. On the other hand, 1 was not transported, suggesting no absorption and no efflux of this compound in Caco-2 cells.

An UPLC-MS/MS Method for Determination of Vicenin-2 and Lychnopholic Acid in Rat Plasma and its Application to a Pharmacokinetic Study

“…VIC and LA were isolated from L. salicifolia leaves as described before. 14 The internal standard (IS) coumarin was purchased from Sigma (USA).…”

Histolocalization of chemotaxonomic markers in Brazilian Vernonieae (Asteraceae)

“…It is well know that Lychnophorinae produce metabolites of therapeutic importance, such as terpenoids (mono‐, sesqui‐, di‐ and triterpenes and sesquiterpene lactones) and phenolic compounds (flavonoids and cinnamic acid derivatives) (Vichnewski & Gilbert, ; Vichnewski et al ., ; Herz et al ., ; Dias Barros et al ., ; Oliveira et al ., ; Grael et al ., ; Dos Santos et al ., ; Kanashiro et al ., ; Taleb‐Contini et al ., ; Silvério et al ., ; Keles et al ., ; Dos Santos et al ., ; Baldin et al ., ; De Souza et al ., ; Abreu et al ., ; Silvério et al ., ; Gouvea et al ., ; Saúde‐Guimarães, Raslan & Oliveira, ). In addition, many studies have investigated the chemotaxonomy of Lychnophorinae because of the chemical richness that characterizes the subtribe (Bohlmann et al ., , b; Herz et al ., ; Bohlmann et al ., ; Zdero et al ., ; Bohlmann et al ., ; Bohlmann & Jakupovic, ; Borella et al ., ; Lunardello et al ., ; Borella et al ., ; Vichnewski et al ., ; Sacilotto, Sartori & Vichnewski, ; Sartori et al ., ; Sakamoto et al ., , ; Keles et al ., ; Sakamoto et al ., ; Souza et al ., ; Lusa et al ., ).…”

Biogenesis, characterization, and the effect of vicenin‐gold nanoparticles on glucose utilization in 3T3‐L1 adipocytes: A bioinformatic approach to illuminate its interaction with PTP 1B and AMPK

“…It is well known that type 2 diabetes is due to several reasons including defect in impaired GLUT 4 vesicle translocation from the cytosolic phase to cell membrane. It has been shown that the VN transport is mediated by GLUT 4 vesicle, (i.e), VN utilizes GLUT 4 system for its intracellular transport . In addition, a recent report has shown that VN inhibits various enzymes linked with diabetes including PTP1B in vitro .…”

“…It has been shown that the VN transport is mediated by GLUT 4 vesicle, (i.e), VN utilizes GLUT 4 system for its intracellular transport. 12,13 In addition, a recent report has shown that VN inhibits various enzymes linked with diabetes including PTP1B in vitro. However, the study observed a low PTP1B inhibitory activity than the standard, urosolic acid.…”

“…It is widely distributed in various plant sources, including fenugreek and shows antioxidant and anticancer activities . The active absorption of VN and other glucosylated flavonoids is mediated via glucose transporter, suggesting that the GLUT 4 system is necessary for its cellular transport. However, there is no report about the antidiabetic effect of VN.…”