2018
DOI: 10.5511/plantbiotechnology.18.0525a
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Abstract: Quantitative real-time PCR (qRT-PCR) is widely used to analyze the expression profiles of the genes of interest. In order to obtain accurate quantification data, normalization by using reliable internal control genes is essential. In this study, we evaluated the stability and applicability of eight internal control gene candidates for analyzing gene expression during fruit development in dwarf tomato cultivar Micro-Tom. We collected seventeen different samples from flowers and fruits at different developmental… Show more

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Cited by 8 publications
(66 citation statements)
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References 31 publications
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“…The relative transcript abundance calculation utilized the comparative CT method as described previously [96] using ΔCT of WT under HS as a subtrahend factor in the ΔΔCT subtraction formula for comparison with the phy mutants as follows: ΔΔCT (ΔCT − ΔCT, WT (stress)). The tomato EXPRESSED gene was an endogenous control for gene expression analyses [97].…”
Section: Rna Isolation and Quantitative Rt-pcrmentioning
confidence: 99%
“…The relative transcript abundance calculation utilized the comparative CT method as described previously [96] using ΔCT of WT under HS as a subtrahend factor in the ΔΔCT subtraction formula for comparison with the phy mutants as follows: ΔΔCT (ΔCT − ΔCT, WT (stress)). The tomato EXPRESSED gene was an endogenous control for gene expression analyses [97].…”
Section: Rna Isolation and Quantitative Rt-pcrmentioning
confidence: 99%