Evaluation of interleukin-6 and its soluble receptor components sIL-6R and sgp130 as markers of inflammation in inflammatory bowel diseases

Nikolaus, Susanna; Waetzig, Georg H.; Butzin, Sven; Ziolkiewicz, Monika; Al-Massad, Natalie; Thieme, Florian; Lövgren, Ulf; Rasmussen, Birgitte; Reinheimer, Torsten; Seegert, Dirk; Rosenstiel, Philip; Szymczak, Silke; Schreiber, Stefan

doi:10.1007/s00384-018-3069-8

Cited by 41 publications

(37 citation statements)

References 54 publications

(70 reference statements)

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“…We found serum IL-6 significantly higher in CD patients with active than inactive disease ( Table I ). The ratio of median serum IL-6 in active and inactive CD of 2.12 was similar to that reported by Mavropoulou et al [ 25 ], but lower than in a study by Nikolaus et al [ 24 ]. Diagnostic performance of IL-6 in differentiating active and inactive disease was in our study worse than that of CRP ( Table II ).…”

Section: Discussionsupporting

confidence: 88%

Serum inflammatory markers in the diagnosis and assessment of Crohn’s disease activity

et al. 2021

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IntroductionThe aim of our study was to evaluate the diagnostic characteristics of selected inflammatory markers and the results of multiplication of their concentrations in the diagnosis and assessment of Crohn’s disease (CD) activity.Material and methodsWe studied 49 patients with CD and 31 healthy controls. The CD patients were assigned to subgroups with active and inactive disease based on the Crohn’s Disease Activity Index score. Serum interleukins and C-reactive protein (CRP) were measured using immunoassays.ResultsSerum CRP and interleukins: IL-6, IL-17A, IL-23 were significantly higher in the CD group than in controls, with the best diagnostic performance for IL-23. Only serum IL-6 and CRP were significantly higher in active than in inactive disease, with the better performance of CRP. Multiplication results did not perform better than individual multipliers.ConclusionsSerum CRP may be useful in the assessment of CD activity and there is a need for introduction of IL-23 for the CD diagnosis.

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Section: Discussionsupporting

confidence: 88%

Serum inflammatory markers in the diagnosis and assessment of Crohn’s disease activity

et al. 2021

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“…There have been many experimental evidence suggesting that various inflammatory mediators or markers, such as tumor necrosis factor- α (TNF- α ), interleukin-1 β (IL-1 β ), and interleukin-6 (IL-6), are substantially elevated in LPS-stimulated macrophagocytes [ 13 – 15 ]. IL-6, a central proinflammatory cytokine that is considered a key hallmark of inflammation in the body, is known to be promptly produced in response to infectious factors and tissue injuries and can regulate intracellular signal transduction and affect other cytokines [ 16 – 19 ]. Most importantly, the acute expression of IL-6 has been reported to be a critical driving factor for many diseases [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Astragalus membranaceusInjection Suppresses Production of Interleukin-6 by Activating Autophagy through the AMPK-mTOR Pathway in Lipopolysaccharide-Stimulated Macrophages

Zhang

Tang

Yang

et al. 2020

Oxidative Medicine and Cellular Longevity

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Astragalus membranaceus (AM), used in traditional Chinese medicine, has been shown to enhance immune functions, and recently, its anti-inflammatory effects were identified. However, the mechanisms of action remain unclear. Most studies have shown that autophagy might be involved in the immune response of the body, including inflammation. Here, we developed an inflammatory model by stimulating macrophages with lipopolysaccharides (LPS) to explore the anti-inflammatory effect and mechanisms of AM injection from the perspective of the regulation of autophagy. Immunoblot, immunofluorescence, and ELISA were used to determine the effects of AM injection on the production of interleukin-6 (IL-6) and alterations of autophagy markers. It was found that AM injection reduced the expression of IL-6 in LPS-stimulated macrophages and reversed the LPS-induced inhibition of cellular autophagy. After treatment with inhibitors of signaling pathways, it was shown that LPS downregulated autophagy and upregulated the production of IL-6 in macrophages via the protein kinase B (Akt)/mammalian target of rapamycin (mTOR) pathway. AM injection reversed the effects of LPS by activating the AMP-activated protein kinase (AMPK) instead of inhibiting Akt. These results were further confirmed by testing activators and siRNA silencing of AMPK. Hence, these 2 distinct signaling molecules appear to exert opposite effects on mTOR, which integrates information from multiple upstream signaling pathways, negatively regulating autophagy. In addition, we demonstrated that autophagy might play a key role in regulating the production of IL-6 by testing activators and inhibitors of autophagy and siRNA silencing of ATG5. These findings showed that AM injection might enhance autophagy by activating AMPK and might further play a repressive effect on the LPS-stimulated expression of IL-6. This study explored the relationship between autophagy, signaling pathways, and the production of inflammatory factors in a model of endotoxin infection and treatment with AM injection.

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“…Another study showed that brosis depends on IL-6 (13) , which increases rapidly under stress, and IL-6 is almost always increased in chronic in ammation (14) . In this study, IL-6 in BO group was signi cantly higher than that in non BO group.…”

Section: Discussionmentioning

confidence: 99%

Bronchiolitis obliterans after severe adenovirus pneumonia:a review of 46 cases

Lan¹,

Yu²,

Lin³

et al. 2020

Preprint

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Background:This study aimed to investigate the risk factors of bronchiolitis obliterans caused by severe adenovirus pneumonia. Methods: The First Affiliated Hospital of Xiamen University in January, 2019 was collected The clinical data of 229 children with severe adenovirus pneumonia from January to January 2020 were divided into obliterative bronchiolitis group (BO group) and non obstructive bronchiolitis group (non BO group) according to the follow-up clinical manifestations and imaging data. The clinical data, laboratory examination and imaging data of the children were retrospectively analyzed. Results: Among 229 children with severe adenovirus pneumonia, 46 cases were in BO group. The number of days of hospitalization, oxygen consumption time, LDH, IL-6, AST, D-dimer and hypoxemia in BO group were significantly higher than those in non BO group; The difference was statistically significant (P < 0.05). Univariate logistic regression analysis showed that there were significant differences in the blood routine neutrophil ratio, platelet level, Oxygen supply time, hospitalization days, AST level, whether there was hypoxemia, timing of using hormone, more than two bacterial feelings were found in the two groups, levels of LDH, albumin and Scope of lung imaging (P < 0.05). Conclusions: The related risk factors of BO caused by severe adenovirus pneumonia were long hospitalization days and oxygen consumption time, elevated platelet levels, AST and LDH levels in laboratory tests, and more than two kinds of bacterial infections, double lung involvement, and hypoxemia. Early use of glucocorticoid had a significant effect on reducing the formation of BO.

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Evaluation of interleukin-6 and its soluble receptor components sIL-6R and sgp130 as markers of inflammation in inflammatory bowel diseases

Cited by 41 publications

References 54 publications

Serum inflammatory markers in the diagnosis and assessment of Crohn’s disease activity

Serum inflammatory markers in the diagnosis and assessment of Crohn’s disease activity

Astragalus membranaceusInjection Suppresses Production of Interleukin-6 by Activating Autophagy through the AMPK-mTOR Pathway in Lipopolysaccharide-Stimulated Macrophages

Bronchiolitis obliterans after severe adenovirus pneumonia:a review of 46 cases

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