Evaluation of iNSiGHT VET DXA (Dual-Energy X-ray Absorptiometry) for assessing body composition in obese rats fed with high fat diet: a follow-up study of diet induced obesity model for 8 weeks

Yeu, Jungyun; Ko, Han Jik; Kim, Doyeun; Ahn, Youngbok; Kim, Jae‐Jin; Lee, Wonhee; Jung, IlSin; Suh, Jungkyu; Lee, Seok Jong

doi:10.1186/s42826-019-0004-2

Cited by 10 publications

(6 citation statements)

References 8 publications

(6 reference statements)

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“…DXA was performed in accordance with previous reports [ 16 , 17 ]. The body composition of each mouse was measured using a cone-beam flat-panel DXA detector (iNSiGHT VET DXA, Osteosys, Seoul, Korea).…”

Section: Methodsmentioning

confidence: 99%

Noninvasive monitoring of muscle atrophy and bone metabolic disorders using dual-energy X-ray absorptiometry in diabetic mice

Kishi

Goto

TSURU³

et al. 2023

Exp. Anim.

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Tracking metabolic changes in skeletal muscle and bone using animal models of diabetes mellitus (DM) provides important insights for the management of DM complications. In this study, we aimed to establish a method for monitoring changes in body composition characteristics, such as fat mass, skeletal muscle mass (lean mass), bone mineral density, and bone mineral content, during DM progression using a dual-energy X-ray absorptiometry (DXA) system in a mouse model of streptozotocin (STZ)-induced type 1 DM. In the DM model, STZ administration resulted in increased blood glucose levels, increased water and food intake, and decreased body weight. Serum insulin levels were significantly decreased on day 30 of STZ administration. The DXA analysis revealed significant and persistent decreases in fat mass, lower limb skeletal muscle mass, and bone mineral content in DM mice. We measured tibialis anterior (TA) muscle weight and performed a quantitative analysis of tibial microstructure by micro-computed tomography imaging in DM mice. The TA muscle weight of DM mice was significantly lower than that of control mice. In addition, the trabecular bone volume fraction, trabecular thickness, trabecular number, and cortical thickness were significantly decreased in DM mice. Pearson’s product-moment correlation coefficient analysis showed a high correlation between the DXA-measured and actual body composition. In conclusion, longitudinal measurement of body composition changes using a DXA system may be useful for monitoring abnormalities in muscle and bone metabolism in animal models of metabolic diseases such as DM mice.

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Section: Methodsmentioning

confidence: 99%

Noninvasive monitoring of muscle atrophy and bone metabolic disorders using dual-energy X-ray absorptiometry in diabetic mice

Kishi

Goto

TSURU³

et al. 2023

Exp. Anim.

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“…Mice were randomly divided into four groups after adapting to the laboratory environment for 1 week while being and were given ad libitum access to water and food. Then, the high fat diet (HFD) group was given a diet consisting of 45–60% fat for 12 weeks, which has been previously established as an HFD-induced model of obesity [ 7 , 8 ]. In this study, the Control (Con) group and Control + Exercise (Con + Ex) group were fed a standard chow diet (CD) with 12% kcal fat.…”

Section: Methodsmentioning

confidence: 99%

Aerobic exercise attenuates high-fat diet–induced renal injury through kidney metabolite modulation in mice

Xiong,

Luan,

Yuan

et al. 2024

Renal Failure

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Purpose To investigate the preventive effect of aerobic exercise on renal damage caused by obesity. Methods The mice in the Control (Con) and Control + Exercise (Con + Ex) groups received a standard chow diet for the 21-week duration of the study, while the High-fat diet (HFD) group and High-fat diet + Exercise (HFD + Ex) group were fed an HFD. Mice were acclimated to the laboratory for 1 week, given 12 weeks of being on their respective diets, and then the Con + Ex and HFD + Ex groups were subjected to moderate intensity aerobic treadmill running 45 min/day, 5 days/week for 8 weeks. Results We found that HFD-induced obesity mainly impacts kidney glycerin phospholipids, glycerides, and fatty acyls, and aerobic exercise mainly impacts kidney glycerides, amino acids and organic acids as well as their derivatives. We identified 18 metabolites with significantly altered levels that appear to be involved in aerobic exercise mediated prevention of HFD-induced obesity and renal damage, half of which were amino acids and organic acids and their derivatives. Conclusion Aerobic exercise rewires kidney metabolites to reduce high-fat diet-induced obesity and renal injury.

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“…The mice were scanned using DXA (iNSiGHT VET, Osteosys, Seoul, Republic of Korea) under anesthesia before dissection. Body composition was calculated using an embedded DXA algorithm [23].…”

Section: Body Composition Analysismentioning

confidence: 99%

Chronic Administration of Exogenous Lactate Increases Energy Expenditure during Exercise through Activation of Skeletal Muscle Energy Utilization Capacity in Mice

Jang,

Kyun,

Hwang

et al. 2024

Metabolites

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We compared the effects of chronic exogenous lactate and exercise training, which influence energy substrate utilization and body composition improvements at rest and during exercise, and investigated the availability of lactate as a metabolic regulator. The mice were divided into four groups: CON (sedentary + saline), LAC (sedentary + lactate), EXE (exercise + saline), and EXLA (exercise + lactate). The total experimental period was set at 4 weeks, the training intensity was set at 60–70% VO2max, and each exercise group was administered a solution immediately after exercise. Changes in the energy substrate utilization at rest and during exercise, the protein levels related to energy substrate utilization in skeletal muscles, and the body composition were measured. Lactate intake and exercise increased carbohydrate oxidation as a substrate during exercise, leading to an increased energy expenditure and increased protein levels of citrate synthase and malate dehydrogenase 2, key factors in the TCA(tricarboxylic acid) cycle of skeletal muscle. Exercise, but not lactate intake, induced the upregulation of the skeletal muscle glucose transport factor 4 and a reduction in body fat. Hence, chronic lactate administration, as a metabolic regulator, influenced energy substrate utilization by the skeletal muscle and increased energy expenditure during exercise through the activation of carbohydrate metabolism-related factors. Therefore, exogenous lactate holds potential as a metabolic regulator.

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Evaluation of iNSiGHT VET DXA (Dual-Energy X-ray Absorptiometry) for assessing body composition in obese rats fed with high fat diet: a follow-up study of diet induced obesity model for 8 weeks

Cited by 10 publications

References 8 publications

Noninvasive monitoring of muscle atrophy and bone metabolic disorders using dual-energy X-ray absorptiometry in diabetic mice

Noninvasive monitoring of muscle atrophy and bone metabolic disorders using dual-energy X-ray absorptiometry in diabetic mice

Aerobic exercise attenuates high-fat diet–induced renal injury through kidney metabolite modulation in mice

Chronic Administration of Exogenous Lactate Increases Energy Expenditure during Exercise through Activation of Skeletal Muscle Energy Utilization Capacity in Mice

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