Evaluation of insertion-deletion markers suitable for genetic diversity studies and marker-trait correlation analyses in cultivated peanut (Arachis hypogaea L.)

Meng, Shuo; Xl, Yang; Pm, Dang; Sl, Cui; Gj, Mu; Cy, Chen; Lf, Liu

doi:10.4238/gmr.15038207

Cited by 2 publications

(1 citation statement)

References 33 publications

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“…Only four RGAs with different size indels (3-9 bp), representing synonomous and inframe indels, were discovered. In peanuts, expressed genes with indels can be associated with disease resistance [12] and peanut agronomic traits [45]. In rice, a major rice blast resistance gene Pi54 (Pikh), is associated with an NBS-LRR containing protein with a 144-bp insertion/ deletion (indel) [46].…”

Section: Discussionmentioning

confidence: 99%

Identification of expressed R-genes associated with leaf spot diseases in cultivated peanut

et al. 2018

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Peanut (Arachis hypogaea L.) is an important food and oilseed crop worldwide. Yield and quality can be significantly reduced by foliar fungal diseases, such as early and late leaf spot diseases. Acceptable levels of leaf spot resistance in cultivated peanut have been elusive due to environmental interactions and the proper combination of QTLs in any particular peanut genotype. Resistance gene analogs, as potential resistance (R)-genes, have unique roles in the recognition and activation of disease resistance responses. Novel R-genes can be identified by searches for conserved domains such as nucleotide binding site, leucine rich repeat, receptor like kinase, and receptor like protein from expressed genes or through genomic sequences. Expressed R-genes represent necessary plant signals in a disease response. The goals of this research are to identify expressed R-genes from cultivated peanuts that are naturally infected by early and late spot pathogens, compare these to the closest diploid progenitors, and evaluate specific gene expression in cultivated peanuts. Putative peanut R-genes (381) were available from a public database (NCBI). Primers were designed and PCR products were sequenced. A total of 214 sequences were produced which matched to proteins with the corresponding R-gene motifs. These R-genes were mapped to the genome sequences of Arachis duranensis and Arachis ipaensis, which are the closest diploid progenitors for tetraploid cultivated peanut, A. hypogaea. Identification and association of specific gene-expression will elucidate potential disease resistance mechanism in peanut and may facilitate the selection of breeding lines with high levels of leaf spot resistance.

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Section: Discussionmentioning

confidence: 99%

Identification of expressed R-genes associated with leaf spot diseases in cultivated peanut

et al. 2018

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AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought

Liu

et al. 2018

Sci Rep

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Peanut is an important edible oil crop plant whose quality and yield are greatly affected by drought. The process and molecular mechanisms of recovery from drought are also critical to its productivity, but are currently poorly characterized. Here, we investigate the involvement of peanut AhGLK1 in recovery from drought, and in particular its relationship with AhPORA, which encodes a key enzyme in chlorophyll biosynthesis. We found that chlorophyll content, chlorophyll fluorescence, AhPORA protein level and genes related to chlorophyll biosynthesis and photosynthesis declined markedly under drought conditions, but all increased during recovery. Consistent with this, AhGLK1 expression decreased during water stress and increased when the stress was removed. When AhGLK1 was transformed into Arabidopsis glk1glk2 mutant, it increased the survival rate of the mutant during recovery from drought and fully rescued the mutant’s pale-green phenotype. In addition, chlorophyll content and fluorescence, and the expression of genes related to chlorophyll biosynthesis and photosynthesis, were all increased. Bioinformatics analysis and experimental evidence suggested that AhGLK1 augments the expression of AhPORA by binding to its promoter. Our findings confirm that AhGLK1 plays a role as a transcription factor that upregulates expression of AhPORA during post-drought recovery, thereby stimulating chlorophyll biosynthesis and photosynthesis.

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Evaluation of insertion-deletion markers suitable for genetic diversity studies and marker-trait correlation analyses in cultivated peanut (Arachis hypogaea L.)

Cited by 2 publications

References 33 publications

Identification of expressed R-genes associated with leaf spot diseases in cultivated peanut

Identification of expressed R-genes associated with leaf spot diseases in cultivated peanut

AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought

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