Evaluation of insecticidal activity of a bacterial strain, Serratia sp. EML-SE1 against diamondback moth

Jeong, Hyung Uk; Mun, Hye Yeon; Oh, Hyung Keun; Kim, Seung Bum; Yang, Kwang-Yeol; Kim, Iksoo; Lee, Hyang Burm

doi:10.1007/s12275-010-0221-9

Cited by 25 publications

(11 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liquid culture of Serratia sp. EML-SE1 uses as a biocontrol agent to control the diamondback moth, Plutella xylostella (Jeong et al 2010). These combined results demonstrate that this species acts as a bioinsecticidal agent.…”

Section: Discussionmentioning

confidence: 75%

Prodigiosin produced by Serratia marcescens NMCC46 as a mosquito larvicidal agent against Aedes aegypti and Anopheles stephensi

Patil

Salunke

et al. 2011

Parasitol Res

View full text Add to dashboard Cite

Microbial control agents offer alternatives to chemical pest control as they can be more selective than chemical insecticides. The present study evaluates the mosquito larvicidal potential of microbial pigment prodigiosin produced by Serratia marcescens NMCC46 against Aedes aegypti and Anopheles stephensi. The pigment of S. marcescens NMCC46 was extracted after 24 h from mannitol containing nutrient broth media. The effects of crude extracted pigment on the growth, survival, development, and other life cycle aspects were studied. The LC(50) and LC(90) values of second, third, and fourth instars of A. aegypti (LC(50) = 41.65, 139.51, 103.95; LC(90) = 117.81, 213.68, 367.82) and A. stephensi (LC(50) = 51.12, 105.52, 133.07; LC(90) = 134.81, 204.45, 285.35) were determined. At higher concentration (500 ppm), mortality starts within first 6 h of exposure. More than 50% mortality occurs within the first 24 h. The overall observed effects against A. aegypti and A. stephensi larvae after 48 h were increasing percent survival larvae, survival pupation, adult emergence with decreasing crude pigment extract concentration. These ensure that the resultant mosquito population reduction is substantial even where the larvicidal potential is minimal. The UV (λ (max) = 536 nm), TLC (Rf = 0.9), HPLC, and FTIR analysis of crude pigment shows the presence of prodigiosin as active compound. Thus, the active compound produced by this species would be more useful against vectors responsible for diseases of public health importance. This is the first report on mosquito larvicidal activity of prodigiosin produced by Serratia species.

show abstract

Section: Discussionmentioning

confidence: 75%

Prodigiosin produced by Serratia marcescens NMCC46 as a mosquito larvicidal agent against Aedes aegypti and Anopheles stephensi

Patil

Salunke

et al. 2011

Parasitol Res

View full text Add to dashboard Cite

show abstract

“…In the larval gut of other lepidopterous insects, such as the small white butterfly (Pieris rapae L.), Proteobacteria was the most highly represented phylum (Robinson et al, 2010). Because red or pink colored Serriatia were lethal to insect pests when ingested, these dominant bacterial species even could kill their host insect and might be used as an insecticide (Jeong et al, 2010). The larval diet influenced the microbial composition of these lepidopteran insects (Brinkmann et al, 2008;Pinto-Tomás et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Bacteria abundance and diversity of different life stages of Plutella xylostella (Lepidoptera: Plutellidae), revealed by bacteria culture‐dependent and PCR‐DGGE methods

et al. 2014

View full text Add to dashboard Cite

Microbial abundance and diversity of different life stages (fourth instar larvae, pupae and adults) of the diamondback moth, Plutella xylostella L., collected from field and reared in laboratory, were investigated using bacteria culture-dependent method and PCR-DGGE analysis based on the sequence of bacteria 16S rRNA V3 region gene. A large quantity of bacteria was found in all life stages of P. xylostella. Field population had higher quantity of bacteria than laboratory population, and larval gut had higher quantity than pupae and adults. Culturable bacteria differed in different life stages of P. xylostella. Twenty-five different bacterial strains were identified in total, among them 20 strains were presented in larval gut, only 8 strains in pupae and 14 strains in adults were detected. Firmicutes bacteria, Bacillus sp., were the most dominant species in every life stage. 15 distinct bands were obtained from DGGE electrophoresis gel. The sequences blasted in GenBank database showed these bacteria belonged to six different genera. Phylogenetic analysis showed the sequences of the bacteria belonged to the Actinobacteri, Proteobacteria and Firmicutes. Serratia sp. in Proteobacteria was the most abundant species in larval gut. In pupae, unculturable bacteria were the most dominant species, and unculturable bacteria and Serratia sp. were the most dominant species in adults. Our study suggested that a combination of molecular and traditional culturing methods can be effectively used to analyze and to determine the diversity of gut microflora. These known bacteria may play important roles in development of P. xylostella.

show abstract

“…Jeong et al . showed that S. marcescens produces insecticidal oral toxins [122]. Interestingly, there is a synergistic insecticidal effect between S. marcescens chitinases [123] and Bt Cry1Ac toxin [90].…”

Section: Insecticidal Genes and Toxins Shared By Entomopathogenic mentioning

confidence: 99%

Common Virulence Factors and Tissue Targets of Entomopathogenic Bacteria for Biological Control of Lepidopteran Pests

Castagnola

Stock

2014

Insects

View full text Add to dashboard Cite

This review focuses on common insecticidal virulence factors from entomopathogenic bacteria with special emphasis on two insect pathogenic bacteria Photorhabdus (Proteobacteria: Enterobacteriaceae) and Bacillus (Firmicutes: Bacillaceae). Insect pathogenic bacteria of diverse taxonomic groups and phylogenetic origin have been shown to have striking similarities in the virulence factors they produce. It has been suggested that the detection of phage elements surrounding toxin genes, horizontal and lateral gene transfer events, and plasmid shuffling occurrences may be some of the reasons that virulence factor genes have so many analogs throughout the bacterial kingdom. Comparison of virulence factors of Photorhabdus, and Bacillus, two bacteria with dissimilar life styles opens the possibility of re-examining newly discovered toxins for novel tissue targets. For example, nematodes residing in the hemolymph may release bacteria with virulence factors targeting neurons or neuromuscular junctions. The first section of this review focuses on toxins and their context in agriculture. The second describes the mode of action of toxins from common entomopathogens and the third draws comparisons between Gram positive and Gram negative bacteria. The fourth section reviews the implications of the nervous system in biocontrol.

show abstract

Evaluation of insecticidal activity of a bacterial strain, Serratia sp. EML-SE1 against diamondback moth

Cited by 25 publications

References 27 publications

Prodigiosin produced by Serratia marcescens NMCC46 as a mosquito larvicidal agent against Aedes aegypti and Anopheles stephensi

Prodigiosin produced by Serratia marcescens NMCC46 as a mosquito larvicidal agent against Aedes aegypti and Anopheles stephensi

Bacteria abundance and diversity of different life stages of Plutella xylostella (Lepidoptera: Plutellidae), revealed by bacteria culture‐dependent and PCR‐DGGE methods

Common Virulence Factors and Tissue Targets of Entomopathogenic Bacteria for Biological Control of Lepidopteran Pests

Contact Info

Product

Resources

About