Evaluation of inhibitory effect and feasible utilization of dilute acid-pretreated rice straws on succinate production by metabolically engineered Escherichia coli AS1600a

Jampatesh, Surawee; Sawisit, Apichai; Wong, Nonthaporn; Jantama, Sirima Suvarnakuta; Jantama, Kaemwich

doi:10.1016/j.biortech.2018.11.002

Cited by 20 publications

(16 citation statements)

References 47 publications

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“…The total inhibitors increased from 5.56 g/L to 14.20 g/L when the dry biomass loading range from 5% (w/v) to 35% (w/v). The rice straw pretreated with H 3 PO 4 showed signi cantly lower total inhibitors when compared to those of the pretreatments with H 2 SO 4 and HCl [17]. The total inhibitors of 5.10 g/L were obtained when 1 N H 2 SO 4 pretreated rice straw with a dry biomass loading of 10% (w/v) for 60 min.…”

Section: Sugars and Inhibitors Liberated During Pretreatment Of Cs By Dilute H 2 Somentioning

confidence: 92%

“…A high xylose concentration of 45.22 g/L with glucose concentration of 8.30 g/L and arabinose concentration of 6.07 g/L was obtained at a dry biomass loading of 35% (w/v). This may be due to hemicellulose has a lower molecular weight and is less ligni ed and highly amorphous compared to cellulose, thus it is easily hydrolyzed by acids [17]. The percentages of hemicellulose sacchari cation and cellulose sacchari cation decreased as the increase of corn stover loading during diluted acid pretreatment.…”

Section: Sugars and Inhibitors Liberated During Pretreatment Of Cs By Dilute H 2 Somentioning

confidence: 99%

“…The inhibitors derived from the pretreatment of CS have adverse impact on sacchari cation by cellulase. The effect could be eliminated via the operation of washing, biodetoxi cation, sodium bisul te addition, etc [7,10,17]. However, washing would generate a large quantity of waste water while biodetoxi cation need a long period.…”

Section: Optimization Of Sscf Processmentioning

confidence: 99%

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The Advanced Performance of Microbial Consortium for Simultaneous Utilization of Glucose and Xylose to Produce Lactic Acid Directly from Dilute Sulfuric Acid Pretreated Corn Stover

Sun¹,

Li²,

Wu³

et al. 2021

Preprint

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Background: Lignocellulosic feedstocks have attracted much attention as an alternative carbon source for lactic acid (LA) production with the advantages of ready availability, sustainability, and renewability. However, the production of LA from lignocellulose faces at least two major technical obstacles. The inhibitors derived from pretreatment of lignocellulose inhibit the growth of microorganism used in downstream hydrolysis and fermentation processes. In addition, most LA producers cannot to ferment pentose sugars and have carbon catabolite repression (CCR) effect. Microbial consortium with great robustness can use complex feedstocks displaying high bioconversion efficiency and has received great attention nowadays.Results: in this study, a thermophilic LA producing consortium DUT50 was enriched and employed to enhance the utilization of corn stover (CS). Enterococcus was the dominant family, accounting for 93.66% abundance in DUT50, and the abundance of Lactobacillus, Bacillus, Lactococcus and Trichococcus, accounted for 2.68% in total. This consortium was highly resistant to inhibitors up to 10.90 g/L derived from pretreatment of CS, metabolized hexose and pentose simultaneously without CCR effect. Based on consortium promising features, an efficient process of simultaneous saccharification and co-fermentation (SSCF) was developed to produce LA from acid-pretreated corn stover. The economical route avoided the operations of solid–liquid separation and detoxification. The key influential factors, including dry biomass and cellulase loading, corn steep liquor powder concentration, and the pre-hydrolysis time were investigated and optimized. The highest LA titer of 71.04 g/L with a yield of 0.49 g/g-CS was achieved at a dry biomass loading of 20% (w/v). This is the reported highest LA production from non-detoxified acid-pretreated corn stover via the SSCF process without wastewater generation. The interaction mode of Enterococcus was collaboration while the low abundance of Lactobacillus and Bacillus might metabolize xylose efficiently via the pentose phosphate pathway.Conclusions: Our results demonstrated the potential advantage of symbiosis and provided a feasible and economical route to produce LA from lignocellulosic biomass in industrial scale.

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Section: Sugars and Inhibitors Liberated During Pretreatment Of Cs By Dilute H 2 Somentioning

confidence: 92%

Section: Sugars and Inhibitors Liberated During Pretreatment Of Cs By Dilute H 2 Somentioning

confidence: 99%

Section: Optimization Of Sscf Processmentioning

confidence: 99%

See 1 more Smart Citation

The Advanced Performance of Microbial Consortium for Simultaneous Utilization of Glucose and Xylose to Produce Lactic Acid Directly from Dilute Sulfuric Acid Pretreated Corn Stover

Sun¹,

Li²,

Wu³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Numerous techniques, such as hot water, dilute sulphuric etc., have been utilized to eliminate the recalcitrant lignin in lignocellulosic waste focusing on the feedstock being beneficiated to mitigate environmental pollution. Several lignocellulosic waste pretreatment approaches have been impractical and wasteful due to some technical challenges, which include the low yield and the formation of inhibitory by-products, e.g., furfural (up to 34.5 g/Kg), hydroxymethyl furfural (up to 29.5 g/Kg), total phenolics compounds (up to 4.1 g/Kg) and weak organic acids (up to 114.9 g/Kg), including total furans (up to 34.5 g/Kg) [51,52]. The processes involved bio-physico-chemical ligninolysis and holocellulolysis of lignocellulosic waste include physical (milling), thermal (hot water), chemical (dilute acid, caustic) and microbial-based processes [53,54].…”

Section: Current Beneficiation Of Lignocellulosic Waste Using Different Pretreatment Techniquesmentioning

confidence: 99%

“…The processes involved bio-physico-chemical ligninolysis and holocellulolysis of lignocellulosic waste include physical (milling), thermal (hot water), chemical (dilute acid, caustic) and microbial-based processes [53,54]. Formation of by-product toxicants is usually observed during dilute acid and alkaline pretreatment as lignin is being partially degraded [48,51]; however, it is still assumed to be the ideal pretreatment method for an industrialized approach to date. Some common types of acid used in pretreating lignocellulosic waste from agriculture and forestry include dilute sulphuric (H 2 SO 4 ), phosphoric (H 3 PO 4 ), hydrochloric (HCl), and nitric (HNO 3 ) acid [55].…”

Section: Current Beneficiation Of Lignocellulosic Waste Using Different Pretreatment Techniquesmentioning

confidence: 99%

Lignocellulosic Waste Pretreatment Solely via Biocatalysis as a Partial Simultaneous Lignino-Holocellulolysis Process

et al. 2021

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Human endeavors generate a significant quantity of bio-waste, even lignocellulosic waste, due to rapid industrialization and urbanization, and can cause pollution to aquatic ecosystems, and contribute to detrimental animal and human health because of the toxicity of consequent hydrolysis products. This paper contributes to a new understanding of the lignocellulosic waste bio-pretreatment process from a literature review, which can provide better biorefinery operational outcomes. The simultaneous partial biological lignin, cellulose and hemicellulose lysis, i.e., simultaneous semi-lignino-holocellulolysis, is aimed at suggesting that when ligninolysis ensues, holocellulolysis is simultaneously performed for milled lignocellulosic waste instead of having a sequential process of initial ligninolysis and subsequent holocellulolysis as is currently the norm. It is presumed that such a process can be solely performed by digestive enzyme cocktails from the monkey cups of species such as Nepenthes, white and brown rot fungi, and some plant exudates. From the literature review, it was evident that the pretreatment of milled lignocellulosic waste is largely incomplete, and ligninolysis including holocellulolysis ensues simultaneously when the waste is milled. It is further proposed that lignocellulosic waste pretreatment can be facilitated using an environmentally friendly approach solely using biological means. For such a process to be understood and applied on an industrial scale, an interdisciplinary approach using process engineering and microbiology techniques is required.

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Engineering Escherichia coli for a high yield of 1,3-propanediol near the theoretical maximum through chromosomal integration and gene deletion

Wong

Jantama

2022

Appl Microbiol Biotechnol

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Evaluation of inhibitory effect and feasible utilization of dilute acid-pretreated rice straws on succinate production by metabolically engineered Escherichia coli AS1600a

Cited by 20 publications

References 47 publications

The Advanced Performance of Microbial Consortium for Simultaneous Utilization of Glucose and Xylose to Produce Lactic Acid Directly from Dilute Sulfuric Acid Pretreated Corn Stover

The Advanced Performance of Microbial Consortium for Simultaneous Utilization of Glucose and Xylose to Produce Lactic Acid Directly from Dilute Sulfuric Acid Pretreated Corn Stover

Lignocellulosic Waste Pretreatment Solely via Biocatalysis as a Partial Simultaneous Lignino-Holocellulolysis Process

Engineering Escherichia coli for a high yield of 1,3-propanediol near the theoretical maximum through chromosomal integration and gene deletion

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