Evaluation of inhibitory activities of plant extracts on production of LPS-stimulated pro-inflammatory mediators in J774 murine macrophages

Verma, Nandini; Tripathi, Subhash; Sahu, D. R.; Das, Hasi R.; Das, Rakha H.

doi:10.1007/s11010-009-0263-6

Cited by 65 publications

(51 citation statements)

References 35 publications

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“…Action due à l'extrait fluide, un peu de la teinture mère et de la fraction éthylacétate, de la résine ; action due à la présence de composés diterpéniques dérivés de l'acide grindélique [5] Action antispasmodique Action antispasmodique dans les spasmes induits à l'acétylcholine Activité spasmolytique de l'extrait et de la fraction polyphénolique : acides phénols et flavonoïdes (mais absence d'activité antispasmodique bronchique induite par la sérotonine ou l'histamine in vivo) [5] Effet expectorant Due à la présence de saponosides [5] Action anti-inflammatoire Activité anti-inflammatoire [15] : test à la carragénine, Inhibe l'élastase des neutrophiles (quercétine-3-méthyléther et 6-OH-kaempférol-3,6-diméthyléther) [5,8] Inhibition de 50 % des sécrétions d'IL-1bêta et IL-12. Elle s'oppose préventivement à la translocation nucléaire du (NF-kappa B) induite par le lipopolysaccharide [15] Antiallergique Au niveau de la peau, Grindélia abaisserait le seuil de sensibilité cutanée. Sous Grindélia, des régressions de dermatite due au Rhus toxidodendron ont été observées (poison ivy, poison oak et sumac vénéneux, famille des Anacardiacées) [3] La toxicité de cette plante pour l'Homme est infime [2].…”

Section: Tableau 3 Propriétés Pharmacologiques De Grindelia Squarrosaunclassified

Grindélia: Grindelia squarrosa (Pursh) Dunal (Asteraceae)

2010

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Notes de botanique [17]Code nomenclatural (nn) : BDNFFnn7948. Code taxonomique (nt) : BDNFFnt8495. Série taxonomique Itis : 531211. Grindelia robusta var. angustifolia (Gray) M.A. Lane n'est plus accepté aujourd'hui et se voit remplacé par Grindelia camporum var. camporum Greene, taxon 37470. Classification botaniqueCette classification figure dans le Tableau 1. Répartition géographiqueSelon la United States Department of Agriculture Plants database, Grindelia squarrosa est une plante qui se distribue sur toutes les régions des États-Unis et du Canada en dehors des États de Floride, Alabama, Virginie, Caroline du Sud et Yukon, Alaska, New Brunswick [17].

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Section: Tableau 3 Propriétés Pharmacologiques De Grindelia Squarrosaunclassified

Grindélia: Grindelia squarrosa (Pursh) Dunal (Asteraceae)

2010

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“…It is used as herbal medicine for the treatment of various diseases like gangrene, ulcers, indigestion, and diarrhea (Pullaiah, 2006;Kane, 2006). Recently it has been reported that the bark extract of Salix nigra can be used as anti-inflammatory agent (Verma et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Promising inhibition of krait snake’s venom acetylcholinesterase by <italic>Salix nigra</italic> and its role as anticancer, antioxidant agent

Ahmed

Ahmad

Khan

et al. 2016

IJAR

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Snakebites are considered a neglected tropical disease that affects thousands of people worldwide. The available antivenoms are associated with numerous side effects. As an alternative, researchers have extensively studied the plants in order to obtain some unusual treatment. The current study was conceded to evaluate the effects of salix nigra (Black willow) against krait snake venom acetylcholinesterase and its role as anti-cancer, anti-oxidant agent. Standard protocols of Ellman, Meyer, Gyamfi and Ruch were used for enzyme inhibition, anti-cancer and free radicals scavenging assays respectively. The methanolic extract of Salix nigra proved to possess neutralization properties against krait snake (Bungarus Sindanus) venom acetylcholinesterase. Statistical data of the results showed that Salix nigra extract inhibited the krait venom acetylcholinesterase through concentration dependent manner. Kinetic analysis using Lineweaver Burk plot revealed that Salix nigra caused competitive type of inhibition i.e. k m value increased while V max remain constant with an increase of extract's concentration. The calculated IC 50 value of Salix nigra was found to be 177µg/ml. In DPPH free radical scavenging assay, extract showed good scavenging potential with IC 50 value of 317µg/ml. Similarly, using H 2 O 2 free radicals, the % scavenging of the extract at 100, 250, 500 and 1000µg/ml was 41, 54, 69 and 74% in comparison with ascorbic acid. Moreover, the extract exhibited good cytotoxic activity against brine shrimps in a dose dependent manner. The present work suggests, for the first time, that Salix nigra extract can be used not only as a potent inhibitor of snake venom acetylcholinesterase but also for the eradication of free radicals along with significant anticancer activity.

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“…11 In unstimulated cells, NFjB remains in the cytoplasm as a heterodimer by corporal association with an inhibitory protein, inhibitor jB (IjB). The release of NF-jB from IjB can occur because of numerous external or internal stimuli.…”

Section: Introduction Hmentioning

confidence: 99%

Artemisia asiaticaNakai Attenuates the Expression of Proinflammatory Mediators in Stimulated Macrophages Through Modulation of Nuclear Factor-κB and Mitogen-Activated Protein Kinase Pathways

Kim

Tang

Cha

et al. 2015

Journal of Medicinal Food

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The present study aimed to examine the anti-inflammatory effects and potential mechanism of action of Artemisia asiatica Nakai (A. asiatica Nakai) extract in activated murine macrophages. A. asiatica Nakai extract showed dosedependent suppression of lipopolysaccharide (LPS)-induced nitric oxide, inducible nitric oxide synthase, and cyclooxygenase-2 activity. It also showed dose-dependent inhibition of nuclear factor-jB (NF-jB) translocation from the cytosol to the nucleus and as an inhibitor of NF-jB-alpha phosphorylation. The extract's inhibitory effects were found to be mediated through NF-jB inhibition and phosphorylation of extracellular signal-regulated kinase 1/2 and p38 in LPS-stimulated J774A.1 murine macrophages, suggesting a potential mechanism for the anti-inflammatory activity of A. asiatica Nakai. To our knowledge, this is the first report of the anti-inflammatory effects of A. asiatica Nakai on J774A.1 murine macrophages; these results may help develop functional foods possessing an anti-inflammatory activity.

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Evaluation of inhibitory activities of plant extracts on production of LPS-stimulated pro-inflammatory mediators in J774 murine macrophages

Cited by 65 publications

References 35 publications

Grindélia: Grindelia squarrosa (Pursh) Dunal (Asteraceae)

Grindélia: Grindelia squarrosa (Pursh) Dunal (Asteraceae)

Promising inhibition of krait snake’s venom acetylcholinesterase by <italic>Salix nigra</italic> and its role as anticancer, antioxidant agent

Artemisia asiaticaNakai Attenuates the Expression of Proinflammatory Mediators in Stimulated Macrophages Through Modulation of Nuclear Factor-κB and Mitogen-Activated Protein Kinase Pathways

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