Evaluation of in vivo antitumor effects of low‐frequency ultrasound‐mediated miRNA‐133a microbubble delivery in breast cancer

Ji, Yanlei; Han, Zhen; Shao, Limei; Zhao, Yuehuan

doi:10.1002/cam4.840

Cited by 46 publications

(44 citation statements)

References 19 publications

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“…Numerous miRNAs reportedly function either as oncogenes or anti-oncogenes. Studies have indicated that miRNAs are often aberrantly regulated and play prohibitive or oncogenic roles in breast cancer (16,17). Recently, the clinical significance of miR-1254 has been verified, along with its role in tumorigenesis and progression, including lung cancer, thyroid cancer and oral squamous cell carcinoma (18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-1254 exerts�oncogenic effects by directly targeting RASSF9 in human breast cancer

Chen

Wang

et al. 2018

Int J Oncol

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MicroRNAs (miRNAs or miRs) play crucial roles in human breast cancer. Although miR-1254 has been shown to have oncogenic activity in several cancer types, its biological function in breast cancer and its mechanisms of action remain unclear. In this study, we investigated the role of miR-1254 in human breast cancer and sought to elucidate the relevant underlying mechanisms. We found that miR-1254 expression was markedly increased in breast cancer tissues and cell lines. Additionally, miR-1254 overexpression accelerated breast cancer cell proliferation, cell cycle G1-S phase transition and inhibited apoptosis. Nevertheless, the inhibition of miR-1254 suppressed cell proliferation and induced apoptosis. Further analyses revealed that miR-1254 expression negatively correlated with RASSF9 expression in breast cancer tissues. We verified that RASSF9 was a direct target of miR-1254 using a luciferase reporter assay. The overexpression of miR-1254 reduced the RASSF9 mRNA and protein levels, and the suppression of miR-1254 promoted RASSF9 expression. Notably, the knockdown or overexpression of RASSF9 corroborated the biological effects observed upon miR-1254 overexpression or inhibition. Taken together, these results demonstrate that miR-1254 accelerates breast cancer cell growth by activating the AKT signaling pathway and suppresses apoptosis by inhibiting p53 expression through the targeting of RASSF9. The data indicate that miR-1254 plays a crucial role in human breast cancer, and may represent a novel therapeutic target for this malignancy.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-1254 exerts�oncogenic effects by directly targeting RASSF9 in human breast cancer

Chen

Wang

et al. 2018

Int J Oncol

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“…These methods are used in combination with other methods, such as lipofection, to protect nucleic acid against degradation by nucleases. To increase gene delivery efficiency of sonoporation, microbubbles were shown to be effective [43] and applied for delivery to cancer cells [44,45] and the central nervous system [46,47]. Clinical trials in phases I and II have been reported for the treatment of melanoma [48][49][50] and solid tumors [51].…”

Section: Sonoporation Electroporation and Magnetofectionmentioning

confidence: 99%

Nucleic Acid-Based Therapy: Development of a Nonviral-Based Delivery Approach

Yokoo¹,

Kamimura²,

Kanefuji³

et al. 2019

In Vivo and Ex Vivo Gene Therapy for Inherited and Non-Inherited Disorders

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Gene therapy returns to the center stage of medicine to treat patients with diseases that are unable to be cured with the conventional therapeutic strategies. This development is due to various reasons, including vector development and significant achievement in nextgeneration sequencing. Among the various methodologies of gene therapy, nucleic acidbased therapy has been considered to be promising in various diseases. The development of delivery methods to target cells in vivo, however, remains critical. These include viral vector-based and nonviral vector-based gene delivery methods as well as physical approaches such as hydrodynamic gene delivery (HGD). HGD is a simple and effective in vivo gene transfer method for the functional analysis of therapeutic genes and regulatory elements in small animals. Moreover, this chapter outlines the principle of HGD, gene expression studies in rodents, and recent advances in clinical application of HGD and provides future perspectives in developing a safe and efficient method for nucleic acidbased therapy.

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“…The therapeutic potential of miRNA-based agents has been investigated in in vivo models of many types of cancers [68][69][70][71][72][73][74] . Systemic delivery methods, such as encapsulation of miRNAs in nanoparticles or chemical modification of miRNAs, could be an effective technique for personalized therapies and cancer treatments [68] .…”

Section: Mirnas In Neuroblastoma Differentiation Therapymentioning

confidence: 99%

MicroRNAs in neuroblastoma differentiation and differentiation therapy

Sousares¹,

Partridge²,

Weigum³

et al. 2017

Adv Mod Oncol Res

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Neuroblastoma is one of the most common and aggressive types of pediatric cancers, making up about 7% of all childhood cancers. Neuroblastoma arises from the failure of neural crest cell precursors to differentiate, and inducing cell differentiation is one of the most important treatment approaches for neuroblastoma. MicroRNAs regulate gene expression by performing post-transcriptional gene modification by mainly translational suppression and mRNA degradation. The dysregulation of these molecules has been shown to be related to tumor development, tumor metastasis and drug resistance, and the promise of developing microRNA-based therapeutics for cancers has been demonstrated. Many recent studies have also provided evidence for the involvement of microRNAs in differentiation of neuroblastoma cells, suggesting the potential of developing microRNA-based differentiation therapies for neuroblastoma. Here we review the recent findings on the role of microRNAs in regulating cell differentiation, with a main focus on neuroblastoma cells. The investigations on the therapeutic potential of microRNAs in neuroblastoma therapy and differentiation therapy are also reviewed.

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Evaluation of in vivo antitumor effects of low‐frequency ultrasound‐mediated miRNA‐133a microbubble delivery in breast cancer

Cited by 46 publications

References 19 publications

MicroRNA-1254 exerts�oncogenic effects by directly targeting RASSF9 in human breast cancer

MicroRNA-1254 exerts�oncogenic effects by directly targeting RASSF9 in human breast cancer

Nucleic Acid-Based Therapy: Development of a Nonviral-Based Delivery Approach

MicroRNAs in neuroblastoma differentiation and differentiation therapy

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