Evaluation of in vitro inhibition of mycelial growth of Fusarium solani f. sp. piperis by different products in Brazil

Dâ€TMAddazio, Veronica; Santos, Renata Aparecida Ahnert dos; Leitao, Arthur Salomao Bastos; Silva, Marcelo Barreto da; Fernandes, Adriano A.; Falqueto, Antelmo Ralph

doi:10.5897/ajmr2016.8292

Cited by 3 publications

(1 citation statement)

References 37 publications

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“…Root rot disease in Brazil has been responsible for the elimination of entire plantations of Piper nigrum L., with economic losses of millions of dollars per year and there is no efficient control measure against the pathogen and no disease-resistant cultivars (D'Addazio et al, 2016). The genetic variation between pepper genotypes in Brazil and the degree of susceptibility of currently available cultivars to the disease remain unknown, which makes it difficult to perform genetic control of fusariosis (Castro et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

By-products of Piper aduncum in the control of fusariosis in black pepper plant

Benchimol

Sutton

Bastos

et al. 2017

Agraria

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Fusariosis (Fusarium solani f.sp. piperis) is one of the most damaging diseases of black pepper crop in the Amazon zone and the application of organic materials to the soil may be an alternative to control this disease. Leaf residues of Piper aduncum were evaluated before and after the extraction of essential oil, as additives to the soil inoculated with Fusarium solani, in the survival and morphophysiological behavior of black pepper seedlings. The experiments were conducted at Embrapa Eastern Amazônia, Belém, PA. The effects of the by-products of the distillation of the essential oil of P. aduncum on the survival to fusariosis in black pepper seedlings and the effect of dry and crushed leaves of P. aduncum, preincubated in the soil, in the incidence of fusariosis and in the behavior of black pepper seedlings were evaluated. The addition of solid residues from P. aduncum oil extraction to the soil increased the survival of seedlings by 80% and in the presence of dry and crushed leaves of P. aduncum the survival was 83%. The net photosynthesis of the seedlings increased in the presence of residues of P. aduncum. The residues from the extraction of the essential oil of P. aduncum and its dry and crushed leaves, without oil extraction, have potential for use in the control of fusariosis in black pepper plants.Key words: Fusarium solani f. sp. piperis; Piper nigrum; residues from essential oil extraction Sub-produtos de Piper aduncum no controle da fusariose da pimenteira-do-reino RESUMO A fusariose (Fusarium solani f. sp. piperis) é uma das doenças mais prejudiciais à cultura da pimenteira-do-reino na Amazônia e a aplicação de materiais orgânicos no solo pode ser uma alternativa de controle dessa doença. Avaliou-se os resíduos foliares de Piper aduncum, antes e após a extração do óleo essencial, como aditivos ao solo inoculado com Fusarium solani, na sobrevivência e no comportamento morfofisiológico de mudas de pimenteira-do-reino. Os experimentos foram conduzidos na Embrapa Amazônia Oriental, Belém, PA. Foram avaliados os subprodutos da destilação do óleo essencial de P. aduncum na sobrevivência de mudas de pimenteira-do-reino à fusariose e o efeito de folhas secas e trituradas de P. aduncum, pré-incubadas no solo, na incidência de fusariose e no comportamento de mudas de pimenteira-do-reino. A adição de resíduos sólidos da extração de óleo de P. aduncum ao solo aumentou a sobrevivência de mudas à fusariose em 80% e na presença de folhas secas e trituradas de P. aduncum o incremento médio na sobrevivência foi de 83%. A fotossíntese líquida das mudas aumentou na presença dos resíduos de P. aduncum. Os resíduos da extração do óleo essencial de P. aduncum e de suas folhas secas e trituradas, sem extração do óleo, tem potencial de uso no controle da fusariose da pimenteira-do-reino. Palavras-chave:Fusarium solani f. sp. piperis; Piper nigrum; resíduos da extração de óleo essencial

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Section: Introductionmentioning

confidence: 99%

By-products of Piper aduncum in the control of fusariosis in black pepper plant

Benchimol

Sutton

Bastos

et al. 2017

Agraria

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Synthesis of Eugenol Derivatives and Evaluation of their Antifungal Activity Against Fusarium solani f. sp. piperis

Maximino

Dutra

Rodrigues

et al. 2020

CPD

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Background: Fusarium solani f. sp. piperis is a phytopathogen that causes one of the most destructive diseases in black pepper crops, resulting in significant economic and crop production losses. Consequently, the control of this fungal disease is a matter of current and relevant interest in agriculture. Objective: The objective was to synthesize eugenol derivatives with antifungal activity. Methods: In this study, using bimolecular nucleophilic substitution and click chemistry approaches, four new and three known eugenol derivatives were obtained. The eugenol derivatives were characterized and their antifungal and cytotoxic effects were evaluated. Results: Eugenol derivative 4 (2-(4-allyl-2-methoxyphenoxy)-3-chloronaphthalene-1,4-dione) was the most active against F. solani f. sp. piperis and showed acceptable cytotoxicity. Compound 4 was two-fold more effective than tebuconazole in an antifungal assay and presented similar cytotoxicity in macrophages. The in silico study of β-glucosidase suggests a potential interaction of 4 with amino acid residues by a cation-π interaction with residue Arg177 followed by a hydrogen bond with Glu596, indicating an important role in the interactions with 4, justifying the antifungal action of this compound. In addition, the cytotoxicity after metabolism was evaluated as a mimic assay with the S9 fraction in HepG2 cells. Compound 4 demonstrated maintenance of cytotoxicity, showing IC50 values of 11.18 ± 0.5 and 9.04 ± 0.2 μg mL-1 without and with the S9 fraction, respectively. In contrast, eugenol (257.9 ± 0.4 and 133.5 ± 0.8 μg mL-1), tebuconazole (34.94 ± 0.2 and 26.76 ± 0.17 μg mL-1) and especially carbendazim (251.0 ± 0.30 and 34.7 ± 0.10 μg mL-1) showed greater cytotoxicity after hepatic biotransformation. Conclusion: The results suggest that 4 is a potential candidate for use in the design of new and effective compounds that could control this pathogen.

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Effect and Compatibility of Phosphite with Trichoderma sp. Isolates in the Control of the Fusarium Species Complex Causing Pokkah Boeng in Sugarcane

Solis-Palacios¹,

Ramírez

Salinas‐Ruíz³

et al. 2021

Agronomy

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Sugarcane, a highly productive crop, is frequently challenged by different biotic agents, such as the pokkah boeng disease that can cause drastic yield losses of up to 40%. This airborne fungal disease is caused by various Fusarium species integrated into a complex. Integrating novel compounds and biological control agents is of paramount importance to cope with these fungi in sustainable systems. In this study, we aimed to evaluate the effect and compatibility of phosphite (Phi) and Trichoderma sp. in the control of Fusarium sp., in vitro and in planta. Using in vitro tests, we evaluated the effects of Phi (0, 500, 1000, 2000, 4000, and 8000 µg mL−1) and the compatibility of Phi + Trichoderma (isolates Taz 001, 013, and 016) on the pathogen complex. Using in planta tests, we evaluated the compatibility of Phi + Trichoderma (Taz-016) in the control of Fusarium in sugarcane plants under greenhouse conditions. A synergistic effect of Phi + Trichoderma was observed in vitro on the control of the pathogen, especially when combining 1000 µg mL−1 + Trichoderma Taz-016. In the in planta trial, combining 4000 µg mL−1 Phi + Trichoderma Taz-016 showed the best control of Fusarium infection, improving plant height, culm length and leaf dry weight.

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Evaluation of in vitro inhibition of mycelial growth of Fusarium solani f. sp. piperis by different products in Brazil

Cited by 3 publications

References 37 publications

By-products of Piper aduncum in the control of fusariosis in black pepper plant

By-products of Piper aduncum in the control of fusariosis in black pepper plant

Synthesis of Eugenol Derivatives and Evaluation of their Antifungal Activity Against Fusarium solani f. sp. piperis

Effect and Compatibility of Phosphite with Trichoderma sp. Isolates in the Control of the Fusarium Species Complex Causing Pokkah Boeng in Sugarcane

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