Evaluation of in vitro cytotoxicity and genotoxicity of copper–zinc alloy nanoparticles in human lung epithelial cells

Kumbıçak, Ümit; Çavaş, Tolga; Çinkılıç, Nilüfer; Kumbıçak, Zübeyde; Vatan, Özgür; Yılmaz, Dilek Demirezen

doi:10.1016/j.fct.2014.07.040

Cited by 30 publications

(18 citation statements)

References 51 publications

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“…This result is consistent with previous findings in mammalian cells that nanoparticles induce single/double stranded DNA breaks as well as chromosomal damage using micronucleus, comet and γ-H 2 AX assays. 39 …”

Section: Resultsmentioning

confidence: 99%

Cu Nanoparticles Have Different Impacts in Escherichia coli and Lactobacillus brevis than Their Microsized and Ionic Analogues

et al. 2015

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Copper formulations have been used for decades for antimicrobial and antifouling applications. With the development of nano-formulations of copper that are more effective than their ionic and micron-sized analogs, a key regulatory question is whether these materials should be treated as new or existing materials. To address this issue, here we compare the magnitude and mechanisms of toxicity of a series of Cu species (at concentration ranging from 2–250 µg/mL), including nano Cu, nano CuO, nano Cu(OH)2 (CuPro and Kocide), micro Cu, micro CuO, ionic Cu2+ (CuCl2 and CuSO4) in two species of bacteria (Escherichia coli and Lactobacillus brevis). The primary size of the particles studied ranged from 10 nm to 10 µm. Our results reveal that Cu and CuO nanoparticles (NPs) are more toxic than their micron-sized counterparts at the same Cu concentration, with toxicities approaching that of the ionic Cu species. Strikingly, these NPs showed distinct differences in their mode of toxicity when compared to the ionic and micron-sized Cu, highlighting the unique toxicity properties of materials at the nanoscale. In vitro DNA damage assays reveal that both nano Cu and micron-sized Cu are capable of causing complete degradation of plasmid DNA but electron tomography results show that only nanoformulations of Cu are internalized as intact intracellular particles. These studies suggest that nano Cu at the concentration of 50 µg/mL may have unique genotoxicity in bacteria compared to ionic and micron-sized Cu.

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Section: Resultsmentioning

confidence: 99%

Cu Nanoparticles Have Different Impacts in Escherichia coli and Lactobacillus brevis than Their Microsized and Ionic Analogues

et al. 2015

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“…For example, Cu is thought to be more easily removed from the human body than noble metals (e.g., gold and silver) and hence should be biocompatible, but some studies call this understanding into question. One of these studies has been performed in human lung epithelial cells, in which Cu‐zinc alloy nanoparticles have been found to induce not only chromosomal damage but also damage to single‐ and double‐stranded DNA . Cu(I) oxide nanoparticles have also been reported to display systemic toxicity, partly due to their ability to target mitochondria to activate the activity of caspase‐3/9 in treated cells .…”

Section: Discussionmentioning

confidence: 99%

Development of Copper Nanoclusters for In Vitro and In Vivo Theranostic Applications

2020

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Theranostics refers to the incorporation of therapeutic and diagnostic functions into one material system. An important class of nanomaterials exploited for theranostics is metal nanoclusters (NCs). In contrast to gold and silver NCs, copper is an essential trace element for humans. It can be more easily removed from the body. This, along with the low cost of copper that offers potential large‐scale nanotechnology applications, means that copper NCs have attracted great interest in recent years. The latest advances in the design, synthesis, surface engineering, and applications of copper NCs in disease diagnosis, monitoring, and treatment are reviewed. Strategies to control and enhance the emission of copper NCs are considered. With this synopsis of the up‐to‐date development of copper NCs as theranostic agents, it is hoped that insights and directions for translating current advances from the laboratory to the clinic can be further advanced and accelerated.

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“…TEM technique uses the dry state of the sample to determine its particle size, whereas DLS method implies the measurement of the particle size in the hydrated state. The observed size differences between TEM and DLS results could be due to hydration spheres surrounding the particles in aqueous DLS samples [27]. Since the diameter measured by the light scattering method is a hydrodynamic diameter, which appears always an overestimate due to the strong interactions between solvent and nanoparticles constituents [28].…”

Section: Synthesis and Characterization Of Ov/cs Nanocompositesmentioning

confidence: 98%

Green Synthesis of Oxovanadium(IV)/chitosan Nanocomposites and Its Ameliorative Effect on Hyperglycemia, Insulin Resistance, and Oxidative Stress

Liu

Guo

et al. 2015

Biol Trace Elem Res

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In this paper, the preparation, characterization, and ameliorative effect on high-fat high-sucrose diet-induced hyperglycemia, insulin resistance, oxidative stress in mice of novel oxovanadium(IV)/chitosan (OV/CS) nanocomposites were investigated. The nanobiocomposite was produced by chemical reduction by chitosan and L-ascorbic acid using microwave heating, under environment-friendly conditions, using aqueous solutions, and notably, by using both mediators as reducing and stabilizing agents. In addition, OV/CS nanocomposites were characterized by transmission electron microscopy, energy dispersive spectroscopy, particle size, and zeta potential measurements. In vivo experiments were designed to examine whether the OV/CS nanocomposites would provide additional benefits on oxidative stress, hyperglycemia, and insulin resistance in mice with type 2 diabetes. The results rendered insulin resistant by treating with OV/CS nanocomposites alleviate insulin resistance and improve oxidative stress. Such nanocomposite seem to be a valuable therapy to achieve and/or maintain glycemic control and therapeutic agents in the treatment arsenal for insulin resistance and type 2 diabetes.

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Evaluation of in vitro cytotoxicity and genotoxicity of copper–zinc alloy nanoparticles in human lung epithelial cells

Cited by 30 publications

References 51 publications

Cu Nanoparticles Have Different Impacts in Escherichia coli and Lactobacillus brevis than Their Microsized and Ionic Analogues

Cu Nanoparticles Have Different Impacts in Escherichia coli and Lactobacillus brevis than Their Microsized and Ionic Analogues

Development of Copper Nanoclusters for In Vitro and In Vivo Theranostic Applications

Green Synthesis of Oxovanadium(IV)/chitosan Nanocomposites and Its Ameliorative Effect on Hyperglycemia, Insulin Resistance, and Oxidative Stress

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