Evaluation of In vitro Cr(VI) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr(VI) polluted industrial landfill

Desai, Chirayu; Jain, Kunal; Madamwar, Datta

doi:10.1016/j.biortech.2007.12.046

Cited by 155 publications

(89 citation statements)

References 43 publications

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“…The observation was made with the pH, having the maximum activity at pH 7. Similar observations of the influence of temperature and pH in the enzyme activity have been made in the literature (Pal et al, 2005;Desai et al, 2008). Suziki et al (1992) reported that some bacteria were known to use chromate as a terminal electron acceptor employing membrane bound enzymes, while others use soluble enzymes.…”

Section: Discussionsupporting

confidence: 76%

Treatment of Chromium Pollution by the Reductase Enzyme Generated by a Chromium-Resistant Bacterium Isolated in Dewatering Sludge

Nguema

Luo²,

Mounir³

et al. 2017

IJB

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An efficient Cr (VI)-resistant and reducing bacterial strain was successfully isolated in dewatering sludge collected from a sewage treatment plant, and characterized in vitro Cr (VI) reduction through a reductase enzyme. Phylogenetic analysis using 16S rDNA gene sequencing revealed that the newly isolated strain namely Pf-1 was closely related to Bacillus cereus. The strain almost reduced 0.17 mM Cr (VI) within 24h incubation and the presence of different substrates such as glucose, sucrose, or acetate significantly enhanced the reduction rate of Cr (VI) to Cr (III). However, addition of the same substrate at the stationary phase of the microbial growth increased the reduction rate as well as bacterial growth. Additionally, raising the concentration of thiosulfate in the medium doubled the reduction rate under similar conditions. Assay with different fractions of the cells demonstrated that the reductase enzyme activity was mainly associated with the cytoplasmic fraction. The maximum activity was 23.3 µM h -1 mg -1 protein and was obtained at the Cr (VI) concentration of 2 mM. The promising strain can be efficiently employed for bioremediation of Cr (VI) polluted sites.

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Section: Discussionsupporting

confidence: 76%

Treatment of Chromium Pollution by the Reductase Enzyme Generated by a Chromium-Resistant Bacterium Isolated in Dewatering Sludge

Nguema

Luo²,

Mounir³

et al. 2017

IJB

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“…. Addition of NADH enhanced Cr(VI) reduction (Desai et al 2008). Another bacterium, Ochrobactrum sp.…”

Section: Bacterial Cr(vi) Reductionmentioning

confidence: 96%

Remediation of chromium contaminants using bacteria

Kanmani¹,

Aravind²,

Preston

2011

Int. J. Environ. Sci. Technol.

118

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Chromium contaminants emanating from industrial activities pose a significant threat to human's wellbeing. Chromium (III) and Chromium (VI) are the forms in which they are commonly encountered, of which the trivalent form is relatively benign. Hence, biological reduction of hexavalent chromium has been widely explored by researchers, yielding fruitful outcomes, opening up exciting avenues and also throwing up new challenges. This article attempts to review this area of research. Microbes, especially bacteria capable of Chromium (VI) reduction, belonging to a heterogeneous group have been isolated from contaminated sites. They exhibit plasmid-mediated chromate resistance and the reduction is enzymatically mediated. Reduction studies have been carried out with free and immobilized enzymes as well as whole cells. Experiments have been carried out in specifically designed bioreactors operated in batch and continuous modes. Although significant progress has been made, much needs to be done for its successful in situ application as the organism may not withstand the Chromium concentration or may be impeded by the presence of other toxicants. With molecular engineering, it may be possible to derive strains with improved performance even under stressful field conditions.

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“…Elangovan and his coworkers observed that chromate reductase activity was associated with soluble proteins and not with the membrane fraction. Desai et al (2008b) suggested that a soluble chromate reductase associated with the cytoplasmic membrane catalyzed Cr (VI) reduction by Pseudomonas sp. G1DM21 by transferring initial one electron to Cr (VI) to form an intermediate Cr (V), followed by two electron transfer to Cr (III) formation.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant

Nguema¹,

Luo²,

Lian³

2014

IJB

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Evaluation of In vitro Cr(VI) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr(VI) polluted industrial landfill

Cited by 155 publications

References 43 publications

Treatment of Chromium Pollution by the Reductase Enzyme Generated by a Chromium-Resistant Bacterium Isolated in Dewatering Sludge

Treatment of Chromium Pollution by the Reductase Enzyme Generated by a Chromium-Resistant Bacterium Isolated in Dewatering Sludge

Remediation of chromium contaminants using bacteria

Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant

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