Evaluation of in-vitro Antithrombosis Activity of Lees of Korean Traditional Wine

Abstract

In this study, ethanol and hot water extracts of lees from Korean traditional wine (J-B, J-S, J-Y, J-H, and J-W) were prepared, and their effects on blood coagulation, platelet aggregation, and hemolysis of human red blood cells (hRBCs) were investigated to develop functional food ingredients from lees. The pH and brix of the lees ranged from 3.90 to 4.29 and 5.0 to 27.0 o , respectively, and there was a huge difference in the water and ethanol content among the lees. The nuruk and additives used affected the color and physicochemical properties of lees. The J-W takju made from only rice and traditional nuruk, which has 13 o brix and 1.8% of alcohol, has potential as functional food ingredient. With regard to the extraction yields of lees, higher yields were obtained from J-H, which contains different medicinal plants, in ethanol, followed by J-W, J-B, J-S, and J-Y. Higher extraction yields of lees were obtained from J-S in hot water, followed by J-B, J-W, J-H, and J-Y, respectively. The ethanol extract of J-H and the hot water extract of J-Y had the highest contents of total polyphenol and total flavonoids among the lees extracts. The 10 lees extracts did not show hemolysis activity against hRBCs up to 5 mg/ml. In an anticoagulation activity assay, the ethanol extracts of three yakju lees (J-B, J-S, and J-Y) and the hot water extract of J-W inhibited thrombin activity, whereas the hot water extract of J-B, J-S, and J-H inhibited blood coagulation factors. In an antiplatelet aggregation activity assay, only the J-W takju lees showed significant inhibition activity. Our results suggest that lees from traditional wine had high potential as a novel antithrombosis agent.

Application of the Lees of Domestic Traditional Wine and its Useful Biological Activity

“…1A). 한편, 기존의 보고된 주박의 생리활성으로는 항산화 활성 [14,17,20,21,27]이 가장 많으며, 최근에는 항혈전 [14,15,16,17], 항염증 [7,30,31,34,38], 항균 [14,15,20] 및 암세포 성장억제 활성 [7,8,38]들이 보고되고 있다. 그 외, 항주름 및 미백활성 [26,37], 항당뇨 [10,18], 알러지 감소 [9], 항비만 및 고혈압 억제 활성 [22,25,34]도 알려져 있어(Fig.…”

Evaluation of in-vitro Anti-thrombosis and Anti-oxidation Activity of Lees of Takju (Wookukseng)

“…성 [10,13], 항혈전 [9] 및 항산화 효과 [13,31], 항염증 [23] 및 알러지 억제 효과 [8], 고혈압 [14. 15] 및 혈당 강하효과 [16], 항 당뇨 [11,19,20] 및 비만 억제 효과 [30] 등이 알려져 있으며, 최근에는 주박의 보습 [29], 미백 및 주름개선 효과 [4,20,21,32] 등이 보고되면서 화장품 향장소재로의 이용 가능성에 대 한 연구 [24,28] PAA : Platelet Aggregation Activity. Data are presented as representative result relative of independent three determinations.…”