Evaluation of in Vitro Antioxidant and Anti-Diabetic Potential of Kashmiri Pomegranate (Punica Granatum Linn.) Flower Extract

Abstract

Aim: The aim of the present study was to find the effect of two extracts namely, aqueous and methanolic of Punica granatum L. Flowers on in vitro anti-oxidant and anti-diabetic potential Methods: The antioxidant activity of extracts was monitored using DPPH assay, Reducing power, Nitric oxide radical inhibition assay whereas, phenolic content was estimated using Folin-Ciocalteu,s reagent. In vitro anti diabetic activity was evaluated by their effect on alpha-amylase and non-enzymatic glycosylation of haemoglobin. Alpha-Amylase inhibitory activity of Punica granatum L. Flower extracts were evaluated using Porcine pancreatic alpha-amylase (PPA) with starch as a substrate, whereas, in non-enzymatic glycosylation of haemoglobin Glucose (2%), haemoglobin (0.06%) and Gentamycin (0.02%) solution were prepared in phosphate buffer 0.01 M, pH 7.4, 1 ml each of above solution was mixed. 1 ml of each concentration was added to above mixture. Mixture was incubated in dark at room temperature for 72 hrs. The degree of glycosylation of haemoglobin was measured colorimetrically at 520 nm in which Ascorbic acid was used as standard drug. Results: There was significant anti-oxidant activity. Methanolic extract showed the highest inhibitory activity than aqueous extract. Both the extracts were strong inhibitors of alpha-amylase and non-enzymatic glycosylation of haemoglobin. Discussion: The alpha-amylase and non-enzymatic glycosylation of haemoglobin inhibitory activities of Punica granatum L. Flowers correlated to antioxidant activity and phenolic content of extracts. Conclusion: Punica granatum L. flowers can be effective in treatment of diabetes not only through inhibition of alpha-amylase and non-enzymatic glycosylation of haemoglobin inhibitory activities, but also by its antioxidant effect.

Evaluation of phytochemical contents and antioxidant activity of pomegranate flower

“…Eddebbagh et al (24) found that the phenolic content of 80% methanolic extract of dried pomegranate flower is 90.73 mg GAE/ g of dry weight flowers. Abdolahi et al (16) have found 28 mg GAE/ g dry weight in 70% ethanolic extract and Rashid and Shafi (25) have found 190.50 and 103.81 mg GAE/ g in methanolic and aqueous extracts in dried pomegranate flower. These differences in phenolic contents might be due to location, soil and climate.…”

Chemistry of heterocycles as carbonic anhydrase inhibitors: A pathway to novel research in medicinal chemistry review

“…different kinds of naturally occurring chemical compounds has been explored as hCAs inhibitors [15,16] (Figure 1). Nowadays, synthetic organic compounds bearing heterocycle moiety have also been used as effective CA inhibitors [17][18][19][20] (Figure 2).…”