Evaluation of in vitro antioxidant and brine shrimp lethality activities of different stem extracts of Zizyphus rugosa Lam

Hossain, Md. Sazzad; Uddin, Nizam; Islam, A. F. M. Mahmudul; Hasan, A. H. M. Nazmul; Hasan, Mohammad Raquibul; Khalik, Md. Farhan; Rana, Md. Sohel

doi:10.1007/s11694-015-9253-4

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…The brine shrimp bioassay has been established as a safe, practical and economic method for determination of bioactivities of synthetic compound as well as plant products 1 . In the brine shrimp lethality, bioassay all the extracts showed considerable lethality and seed extract was found most potent.…”

Section: Discussionmentioning

confidence: 99%

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2017

IJPSR

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ABSTRACT:Aegle marmelos(L.), family rutaceae is highly reputed medicinal tree commonly known as the bael. All parts of the plant have medicinal properties and extensively used by the traditional medicine practitioners of Bangladesh in different health ailments like Diabetes, Diarrhea, Jaundice, typhoid. The present study was dedicated to investigate phytochemical and pharmacological properties of ripe fruit, half-ripe fruit, leaf and seed of the plant extracted with methanol solvent. Initial phytochemical screening confirmed the presence of different phytoconstituents including alkaloid, flavonoids, carbohydrate, glycoside, saponin, tannin, glucoside and steroids in different extracts. Antioxidant potential was evaluated using total phenol and total flavonoid contents determination assays, total antioxidant capacity, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay, NO radical scavenging assay, reducing power assessment, CUPRAC (Cupric Reducing Antioxidant Capacity), total alkaloid determination assay. All the plant parts were found to possess moderate amounts of phenolics and flavonoids, expressed as galic acid equivalent (GAE) and quercetin equivalent (QE) respectively. The methanolic extract of half-ripe fruit demonstrated highest phenolic content (6.05 mg/gm GAE) while methanol extract of seed exhibited highest flavonoid content (4.29 mg/gm QE). In addition, the plant parts displayed total antioxidant capacity expressed as ascorbic acid equivalent (AAE) with methanol extract of leaf being the most potent one (10.31 mg/gm AAE). In DPPH radical and NO radical scavenging methods, a dose dependent scavenging of DPPH and NO radical was observed by all the extractives. Highest DPPH radical scavenging was demonstrated by methanol extract of half-ripe fruit with IC 50 value of 251.2 μg/ml whereas IC 50 value of standard ascorbic acid was noted as 18.4 μg/ml. In case of NO radical scavenging method, highest NO radical scavenging was also demonstrated by methanol extract of half-ripe fruit with IC 50 values of 46.364 μg/ml. However in reducing power and CUPRAC assays, methanol extract of half-ripe fruit and leaf were found to exhibit moderate but concentration dependent reducing power respectively. In total alkaloid content determination methanol extract of seed showed the highest alkaloid content expressed as atropine equivalent (4.86 mg/gm).In Brine Shrimp Lethality Bioassay, all the extracts produced dose dependent cytotoxicity effect to brine shrimp nauplii with methanol extract of seed exhibiting highest toxicity having LC 50 value 86.77 μg/ml where standard vincristine sulphate had the LC 50 value of 2.47 μg/ml.

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Section: Discussionmentioning

confidence: 99%

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2017

IJPSR

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“…AE and ME extracts of leaves of Zizyphus lotus appear richer in polyphenols. In a study by Hossain, Uddin and Islam (2015), the extract of Zizyphus lotus contained a total phenolic content of 97.18± 12.81 mg of gallic acid equivalents /g DW. Zizyphus lotus extracts showed the higher flavonoids content (14.88 mg CE/gDW) in the methanolic extract than in the aqueous extract (11.30 mg CE/gDW).…”

Section: Phytochemical Analysismentioning

confidence: 99%

Zizyphus lotus (L.) extracts as prebiotics in the aggregation and adhesion of probiotic and inhibition of pathologic bacteria from patients with colorectal cancer

2020

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The mucosal surfaces of the intestinal tract harbor a complex microbiota. Bacteria enter in contact with intestinal cells and bestow important nutritional, metabolic and protective functions which benefit the host. Various factors are able to alter the balances between different intestinal bacteria. Dysbiosis has been described in various pathologies and metabolic diseases in humans, such as cancer. Colorectal cancer pathology can benefit from gut microbial imbalance. Its overgrowth may lead to acute symptoms. An alternative strategy to restore this balance is the use of plant extracts which exhibit a prebiotic activity by stimulating of probiotic bacteria and antimicrobial activity against pathogenic bacteria colonization. The present study aims to evaluate the in vitro antioxidant and antibacterial activity of phenolic extracts (Aqueous extract "AE" and Methanolic extract "ME" ) from Zizyphus lotus. It aims also to investigate the effect of this extract as a prebiotic on the aggregation of probiotic and inhibitory effects of pathogenic bacteria isolated from faeces of patients with colorectal cancer. Phytochemical analysis of different extracts from Zizyphus lotus by HPLC showed that all are rich in phenolic compounds (225.40 mgAGE/gDW for AE and 63.04 mgAGE/gDW for ME extracts). Moreover, all extracts showed an important antioxidant activity (227 µg/ml for AE and 195 µg/ml for ME). These extracts also exhibited a significant prebiotic effect and antibacterial activity. Characterization of the in vitro effect of the aqueous extract showed that the percentage of autoaggregation and adhesion of probiotic and inhibitory effects of pathogenic bacteria increased in the presence of aqueous extracts.

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Ziziphus rugosa Leaf: Pharmacognostical Characters and Anti-Inflammatory Properties against Carrageenan-Induced Paw Edema

Krishna,

Saravanan,

Jays

2024

Borneo J Pharm

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Ziziphus rugosa belongs to the family Rhamnaceae, which includes many flowering species, primarily trees and shrubs, and sometimes vines. This study aims to describe the pharmacognostic characteristics and potential anti-inflammatory properties of Z. rugosa leaves. The pharmacognostical and preliminary phytochemical studies were performed following standard procedures. Acetone, ethanol, and aqueous extracts were screened for anti-inflammatory potential using the carrageenan-induced paw edema model. Ziziphus rugosa was identified by its evergreen nature, recurved hooks, and drupe-type fruits. Leaves are elliptic/rounded with cordate base exhibiting a dark green glossy upper surface and pubescent lower surface. They have a dorsiventral nature covering trichomes, collenchyma, sclerenchyma patch, and calcium oxalate crystals as a few key histological characters in the transverse section. In contrast, anamocytic stomata, covering trichomes, crystals, and fragments of vessels, are the imperative elements in powder. The extracts contain carbohydrates, alkaloids, glycosides, tannins, saponins, phenolic compounds, proteins, and flavonoids. The acetone extract at 400 and 200 mg/kg displays a maximum inflammation inhibition of 56.96% and 48.77% among the extracts, and the standard diclofenac sodium inhibits inflammation by 65.61% at 24 hours. The altered liver superoxide dismutase, glutathione, and malondialdehyde levels in the positive control group are significantly near normal in the treatment groups. The histopathological studies of treated animals show significant protection against paw and liver tissue damage. Pharmacognostical study outcomes aid in the identification of species along with ascertaining standardization parameters. Further fractionation of acetone extract followed by isolating compounds responsible for the anti-inflammatory activity would provide an alternative to managing inflammation.

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Evaluation of in vitro antioxidant and brine shrimp lethality activities of different stem extracts of Zizyphus rugosa Lam

Cited by 3 publications

References 42 publications

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Zizyphus lotus (L.) extracts as prebiotics in the aggregation and adhesion of probiotic and inhibition of pathologic bacteria from patients with colorectal cancer

Ziziphus rugosa Leaf: Pharmacognostical Characters and Anti-Inflammatory Properties against Carrageenan-Induced Paw Edema

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