Evaluation of immunomodulatory activity of two potential probiotic Lactobacillus strains by in vivo tests

Ren, Dayong; Li, Chang; Qin, Yanqing; Yin, Ronglan; Du, Shouwen; Liu, Hongfeng; Zhang, Yanfang; Wang, Cuiyan; Rong, Fengjun; Jin, Ningyi

doi:10.1016/j.anaerobe.2015.06.008

Cited by 58 publications

(46 citation statements)

References 27 publications

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“…We also demonstrated that these lactic bacteria had an impact on the phagocytic activity of macrophages against extracellular pathogens such as S. aureus and E. coli and intracellular pathogens such as S. typhimurium . In support of these results, a previous study showed that in a murine model supplementing the diet with L. plantarum CGMC 1.557 for 20 days enhanced phagocytic activity in macrophages [37]. In addition, Kausahal et al [38] demonstrated that Lactobacillus acidophilus and Bifidobacterium bifidum improved the phagocytic potential of macrophages in aged mice.…”

Section: Discussionmentioning

confidence: 54%

ProbioticLactobacillusStrains Stimulate the Inflammatory Response and Activate Human Macrophages

Rocha-Ramirez

Pérez-Solano

Castañón-Alonso

et al. 2017

Journal of Immunology Research

183

120

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Lactobacilli have been shown to promote health functions. In this study, we analyzed the mechanism by which four different strains of probiotics affected innate immunity, such as regulation of ROS, cytokines, phagocytosis, bactericidal activity, signaling by NF-κB pp65, and TLR2 activation. The production of ROS was dependent on the concentration and species of Lactobacillus. The results obtained from the tested strains (Lactobacillus rhamnosus GG, L. rhamnosus KLSD, L. helveticus IMAU70129, and L. casei IMAU60214) showed that strains induced early proinflammatory cytokines such as IL-8,TNF-α, IL-12p70, and IL-6. However, IL-1β expression was induced only by L. helveticus and L. casei strains (after 24 h stimulation). Phagocytosis and bactericidal activity of macrophages against various pathogens, such as S. aureus, S. typhimurium, and E. coli, were increased by pretreatment with Lactobacillus. The nuclear translocation NF-κB pp65 and TLR2-dependent signaling were also increased by treatment with the probiotics. Taken together, the experiments demonstrate that probiotic strains of Lactobacillus exert early immunostimulatory effects that may be directly linked to the initial inflammation of the response of human macrophages.

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Section: Discussionmentioning

confidence: 54%

ProbioticLactobacillusStrains Stimulate the Inflammatory Response and Activate Human Macrophages

Rocha-Ramirez

Pérez-Solano

Castañón-Alonso

et al. 2017

Journal of Immunology Research

183

120

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“…It is not yet clear why Lactobacillus , but not Bacteroides , was evident within the spleen, but there are likely multiple factors that contribute. For example, several species of Lactobacillus , including species with probiotic properties, have been shown to stimulate an increase in phagocytosis and to increase bacterial translocation to the mesenteric lymph nodes and Peyer’s patches (58–60). At the same time, it may be more challenging for the innate myeloid cells to break down the Gram-positive cell wall, and subsequently the rRNA, of lactobacilli than it is to break down the Gram-negative cell envelope of Bacteroides .…”

Section: Discussionmentioning

confidence: 99%

Exposure to a Social Stressor Induces Translocation of Commensal Lactobacilli to the Spleen and Priming of the Innate Immune System

Lafuse

Gearinger

Fisher

et al. 2017

The Journal of Immunology

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Studies have shown that exposure to psychological stressors leads to inflammation throughout the body. This has been widely studied using social disruption stress (SDR), a social stressor, which involves repeated social defeat in subordinate mice. Exposure to SDR increases serum cytokine levels, results in accumulation of spleen CD11b+ myeloid cells, and primes macrophages for increased cytokine and microbicidal activity. Our previous studies showed that intestinal microbes are necessary for SDR-enhancement of innate immunity. Here, we show that SDR increases spleen CD11b+Ly6CintermLy6G+ neutrophil and CD11b+Ly6ChiLy6G− monocyte numbers compared to control mice. Further, we found that neutrophils and monocytes from stressor-exposed mice expressed higher levels of IL-1β mRNA. To determine whether bacterial translocation may contribute to these effects, bacterial 16S rRNA was quantified using qRT-PCR with bacterial group-specific primers. Exposure to the SDR stressor specifically increased Lactobacillus RNA in the spleen, which localized in spleen monocytes. The increased spleen levels of Lactobacillus 16S rRNA in SDR mice positively correlated with increased levels of IL-1β and IL-23 mRNA. Our findings indicate that during stressor exposure, Lactobacillus spp. can translocate to the spleen and prime the innate immune system for enhanced reactivity.

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“…Lymphocyte proliferation assay was performed according to Ren et al [44]. Production of cytokines (IFN-α, IFN-γ, IL-10) was measured according to Ren et al [44].…”

Section: Methodsmentioning

confidence: 99%

“…Production of cytokines (IFN-α, IFN-γ, IL-10) was measured according to Ren et al [44]. Gene expression of TLR-2, interferon (IFN)-γ, IL-4, Foxp3 and transforming growth factor (TGF)-β in intestinal mucosa was determined according to Zhu et al [45].…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Fakruddin

Hossain

Ahmed

2017

BMC Complement Altern Med

137

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BackgroundProbiotic yeast has become a field of interest to scientists in recent years.MethodsConventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties.ResultsThis study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme. It can produce organic acid and showed resistance against tetracycline, ampicillin, gentamycin, penicillin, polymixin B and nalidixic acid. It can assimilate cholesterol, can produce killer toxin, vitamin B12, glutathione, siderophore and strong biofilm. It showed moderate auto-aggregation ability and cell surface hydrophobicity. The isolate can produce enzymes such as amylase, protease, lipase, cellulose, but unable to produce galactosidase. The isolate can’t produce gelatinase and DNase. The isolate showed moderate anti-microbial activity against bacteria and fungi and cell lysate showed better antimicrobial activity than whole cell and culture supernatant. Again, the isolate showed better anti-bacterial activity against gram negative bacteria than gram positive. The isolate showed strong antioxidant activity, reducing power, nitric oxide and hydroxyl radical scavenging activity, significant brine shrimp cytotoxicity and acute toxicity and metal ion chelating activity. The isolate did not induce any detectable change in general health of mice upon oral toxicity testing and found to be safe in mouse model. The isolate improve lymphocyte proliferation and cytokine production in treated mice.ConclusionsSuch isolate could be potential as probiotic to be used therapeutically.

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Evaluation of immunomodulatory activity of two potential probiotic Lactobacillus strains by in vivo tests

Cited by 58 publications

References 27 publications

ProbioticLactobacillusStrains Stimulate the Inflammatory Response and Activate Human Macrophages

ProbioticLactobacillusStrains Stimulate the Inflammatory Response and Activate Human Macrophages

Exposure to a Social Stressor Induces Translocation of Commensal Lactobacilli to the Spleen and Priming of the Innate Immune System

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

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