Evaluation of Immunomodulatory Activities of Purified Microcin Produced by Klebsiella Pneumoniae in Experimental Animals

Abdul-Zahraa, Aqeela; Al-Joofy, Ikbal Khudhur; Khelkal, Intesar Nadhum

doi:10.36872/lepi/v50i2/201005

Cited by 2 publications

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“…E. coli was a stronger inducer of IL-production of IL-10 at early stages of acute inflammation may result in inhibition of TNF-and may be responsible for ineffective elimination of infectious agents [24]. The results came in consistence to a study by Abdul-Zahraa et al [25] reported that the administration of microcin to mice, elevated Serum level of TNF-α to 21.86 (pg/ml) when compared to control 14.32(pg/ml) and IL-10 was 27.24 (pg/ml) compared to control 19.54(pg/ml).…”

Section: Discussionsupporting

confidence: 60%

The Role of Microcin B17-Producing Escherichia Coli, Isolated from Patients with Inflammatory Bowel Disease on Rat Intestine

AL-Baroody,

Ahmed,

Alsaadi

2023

Azb Pharm Pharmaco J

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Background: Inflammatory Bowel Disease (IBD) is a chronic gastrointestinal disease, Crohn’s disease (CD) and ulcerative colitis (UC) are two distinct kinds of IBD. Recent researches have linked between Escherichia coli which produce the toxin microcin B17 (E. coli/+mB17) and the development of inflammatory bowel disease (IBD). Objective: This study aimed to demonstrate the relationship between these E. coli isolates and the emergence of IBD in an experimental animal models. Materials and Methods: Twenty female rats, five-weeks-old were divided in to four groups; first group, were orally given E. coli/+mB17 isolated from crohn’s patients stool, the second group rats were orally given E. coli/+mB17 isolated from patients stool with ulcerative colitis, in the third group were orally given E. coli non-producing microcin B17 (E. coli/-mB17) isolated from patients stool with IBD, and the last group received normal saline orally, as control. After fifteen weeks of administration, Blood was drawn for serological tests which include interlukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) and the rats sacrificed for evaluation of histological changes in their intestine. Results: All groups that were administrated bacteria, whether they were toxin producer or not, showed a decrease in their weights with a reduction of appetite. Serological changes observed included an increase in IL-10 level and an increase in TNF-α level in their sera. Regarding the histological examination, there were noticeable histological changes in the large intestines of all rats given bacterial suspension but not in small intestine. Conclusion: All E.coli isolates which were isolated from IBD patients stool (whether it was microcin B17 producing or not) were able to induce inflammatory responses and histological changes in the intestines of rats . Accordingly, other virulence factors (in addition to Microcin B17) may play role in the pathogenesis of IBD.

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Section: Discussionsupporting

confidence: 60%

The Role of Microcin B17-Producing Escherichia Coli, Isolated from Patients with Inflammatory Bowel Disease on Rat Intestine

AL-Baroody,

Ahmed,

Alsaadi

2023

Azb Pharm Pharmaco J

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“…K lebsiella pneumoniae (K. pneumoniae) is a common Gram-negative opportunistic bacterium of the family Enterobacteriaceae that grows optimally at pH (6)(7)(8) and at a temperature of 37°C [1], [2]. It presents as normal flora in the human oropharynx and gastrointestinal mucous membrane, which act as primary reservoirs for K. pneumoniae in humans [3], [4]. It causes hospital-acquired infections such as pneumonia, wound infections, and urinary tract infections (UTIs) as well as communityacquired infections such as liver abscess, bone infections, pneumonia, meningitis, and soft tissue infections [5]- [7].…”

Section: Introductionmentioning

confidence: 99%

The Effect of pH Variation on Antibiotic Susceptibility of MDR <i>Klebsiella pneumoniae</i> Isolates

Hassan,

Khelkal,

Al Marjani

et al. 2024

Al-Mustansiriyah Journal of Science

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Background: Klebsiella pneumoniae is a significant opportunistic pathogen responsible for various nosocomial infections in humans. The emergence of multi-drug resistant strains poses a significant challenge in clinical settings, necessitating a deeper understanding of factors influencing antimicrobial resistance. Objective: This research aimed to investigate the impact of pH variation on the resistance patterns of multi-resistant K. pneumoniae isolated from Iraqi patients with urinary tract infections and wound infections against different antibiotics. Methods: Forty K. pneumoniae isolates were obtained from urine samples and wound swabs, and their identification was confirmed using the VITEK ® 2 compact system and molecular identification of the rpoB housekeeping gene. Antibiotic susceptibility testing was performed using the Kirby Bauer’s disk diffusion method under varying pH conditions (pH 5, 7, 9, and 11) at 37°C for 18 to 24 hours. Results: The study findings indicated that K. pneumoniae isolates exhibited differential susceptibility to antibiotics based on pH conditions. Cefotaxime demonstrated increased efficacy under alkaline pH, while tetracycline showed optimal efficacy under acidic conditions. However, ciprofloxacin displayed resistant phenotypes at acidic pH 5 and either resistant or intermediate phenotypes at alkaline pH 9. Conclusions: The results suggest a potential influence of pH on the antibiotic susceptibility profiles of K. pneumoniae isolates. Understanding the role of pH in antimicrobial resistance can inform strategies for better managing infections caused by multi-resistant pathogens. Further research is warranted to elucidate the underlying mechanisms and implications for clinical practice.

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Evaluation of Immunomodulatory Activities of Purified Microcin Produced by Klebsiella Pneumoniae in Experimental Animals

Cited by 2 publications

References 0 publications

The Role of Microcin B17-Producing Escherichia Coli, Isolated from Patients with Inflammatory Bowel Disease on Rat Intestine

The Role of Microcin B17-Producing Escherichia Coli, Isolated from Patients with Inflammatory Bowel Disease on Rat Intestine

The Effect of pH Variation on Antibiotic Susceptibility of MDR <i>Klebsiella pneumoniae</i> Isolates

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