Evaluation of immunogenicity and protection mediated by Lawsonia intracellularis subunit vaccines

Fourie, Kezia R.; Choudhary, Pooja; Ng, Siew Hon; Obradović, Milan; Brownlie, Robert; Anand, Sanjeev K.; Wilson, Heather L.

doi:10.1016/j.vetimm.2021.110256

Cited by 9 publications

(7 citation statements)

References 86 publications

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“…Mucosal immune response, specifically IgA antibodies are also relevant for this microorganism, which invades the mucous membranes, specifically the digestive system [ 77 , 78 ]. Evaluating the stimulation of these immune responses in pigs is necessary to cover the entire immune response essential for this pathogen control.…”

Section: Discussionmentioning

confidence: 99%

Co-Formulation of Recombinant Porcine IL-18 Enhances the Onset of Immune Response in a New Lawsonia intracellularis Vaccine

Hidalgo-Gajardo,

Gutiérrez,

Lamazares

et al. 2023

Vaccines

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Pig is one of the most consumed meats worldwide. One of the main conditions for pig production is Porcine Enteropathy caused by Lawsonia intracellularis. Among the effects of this disease is chronic mild diarrhea, which affects the weight gain of pigs, generating economic losses. Vaccines available to prevent this condition do not have the desired effect, but this limitation can be overcome using adjuvants. Pro-inflammatory cytokines, such as interleukin 18 (IL-18), can improve an immune response, reducing the immune window of protection. In this study, recombinant porcine IL-18 was produced and expressed in Escherichia coli and Pichia pastoris. The protein’s biological activity was assessed in vitro and in vivo, and we determined that the P. pastoris protein had better immunostimulatory activity. A vaccine candidate against L. intracellularis, formulated with and without IL-18, was used to determine the pigs’ cellular and humoral immune responses. Animals injected with the candidate vaccine co-formulated with IL-18 showed a significant increase of Th1 immune response markers and an earlier increase of antibodies than those vaccinated without the cytokine. This suggests that IL-18 acts as an immunostimulant and vaccine adjuvant to boost the immune response against the antigens, reducing the therapeutic window of recombinant protein-based vaccines.

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Section: Discussionmentioning

confidence: 99%

Co-Formulation of Recombinant Porcine IL-18 Enhances the Onset of Immune Response in a New Lawsonia intracellularis Vaccine

Hidalgo-Gajardo,

Gutiérrez,

Lamazares

et al. 2023

Vaccines

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“…2A ) in the ileum of infected mice indicated that L. intracellularis bacteria regulate the expression of chaperonin during the initial stages of colonization. GroEL (also known as Hsp60 ) is a stress response protein that promotes refolding and proper assembly of polypeptides and undergoes changes in cellular localization from cytosol to cell surface making it possible immunogen [ 29 30 ]. The upregulation of groEL expression was associated with the presence of multiple copies of the L. intracellularis 16S rRNA gene in the mouse ileum.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of host and bacterial gene modulation during Lawsonia intracellularis infection in immunocompetent C57BL/6 mouse model

et al. 2022

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Background Proliferative enteritis caused by Lawsonia intracellularis undermines the economic stability of the swine industry worldwide. The development of cost-effective animal models to study the pathophysiology of the disease will help develop strategies to counter this bacterium. Objectives This study focused on establishing a model of gastrointestinal (GI) infection of L. intracellularis in C57BL/6 mice to evaluate the disease progression and lesions of proliferative enteropathy (PE) in murine GI tissue. Methods We assessed the murine mucosal and cell-mediated immune responses generated in response to inoculation with L. intracellularis . Results The mice developed characteristic lesions of the disease and shed L. intracellularis in the feces following oral inoculation with 5 × l0 7 bacteria. An increase in L. intracellularis 16s rRNA and groEL copies in the intestine of infected mice indicated intestinal dissemination of the bacteria. The C57BL/6 mice appeared capable of modulating humoral and cell-mediated immune responses to L. intracellularis infection. Notably, the expression of genes for the vitamin B12 receptor and for secreted and membrane-bound mucins were downregulated in L. intracellularis -infected mice. Furthermore, L. intracellularis colonization of the mouse intestine was confirmed by the immunohistochemistry and western blot analyses. Conclusions This is the first study demonstrating the contributions of bacterial chaperonin and host nutrient genes to PE using an immunocompetent mouse model. This mouse infection model may serve as a platform from which to study L. intracellularis infection and develop potential vaccination and therapeutic strategies to treat PE.

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“…A resposta imune humoral desencadeada pela vacinação ou pela infecção pode ser diferenciada utilizando vacinas baseadas na estratégia DIVA (Differentiating Infected from Vaccinated Animals), as quais podem ser constituídas de epítopos específicos do microrganismo, gerando anticorpos facilmente diferenciáveis daqueles desenvolvidos contra a célula inteira de L. intracellularis durante a infecção (CAPUA et al, 2003;FOURIE et al, 2021).…”

Section: Diagnóstico E Tratamentounclassified

Lawsonia intracellularis: INFECÇÃO E PROFILAXIA

Conrad,

Mazzoleni,

Costa

et al. 2023

SCI. ANIM. HEALTH

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Lawsonia intracellularis é uma bactéria Gram-negativa intracelular obrigatória que causa a Enteropatia Proliferativa Suína (EPS), uma doença economicamente importante para a suinocultura. A infecção é caracterizada pela hiperplasia da mucosa intestinal, desencadeando sinais clínicos como diarreia, perda de peso e, em alguns casos, morte súbita. A prevalência das infecções em escala mundial é difícil de ser estimada devido a presença de animais assintomáticos e difícil diagnóstico diferencial, tornando incalculáveis as perdas econômicas decorrentes da infecção. A compreensão da patogênese de L. intracellularis, especialmente como o hospedeiro responde à infecção e os fatores que influenciam para os diferentes desfechos clínicos é essencial para que intervenções apropriadas sejam realizadas. A vacinação é a melhor medida profilática contra EPS, contudo as vacinas disponíveis apresentam limitações, motivando pesquisadores à busca de novos antígenos, estratégias e sistemas de entrega vacinais que proporcionem a resposta imune protetora à L. intracellularis. Neste estudo revisamos os principais fatores relacionados à patogenicidade de L. intracellularis e os principais aspectos da resposta vacinal. Além disso, a revisão aborda o cenário de vacinas disponíveis e em desenvolvimento contra L. intracelullaris, incluindo os principais epítopos e estratégias que vem sendo estudadas.

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Evaluation of immunogenicity and protection mediated by Lawsonia intracellularis subunit vaccines

Cited by 9 publications

References 86 publications

Co-Formulation of Recombinant Porcine IL-18 Enhances the Onset of Immune Response in a New Lawsonia intracellularis Vaccine

Co-Formulation of Recombinant Porcine IL-18 Enhances the Onset of Immune Response in a New Lawsonia intracellularis Vaccine

Evaluation of host and bacterial gene modulation during Lawsonia intracellularis infection in immunocompetent C57BL/6 mouse model

Lawsonia intracellularis: INFECÇÃO E PROFILAXIA

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