Evaluation of Immunoassay‐Based MMP‐8 Detection in Gingival Crevicular Fluid on a Point‐of‐Care Platform

Munjal, S. K.; Prescher, N.; Struck, Friedhelm; Sorsa, Timo; Maier, Kurt J.; Netuschil, L

doi:10.1196/annals.1384.018

Cited by 23 publications

(16 citation statements)

References 8 publications

(17 reference statements)

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“…We feel that with the advent of point-of-care devices for measuring MMP expression [26,27], the lessons learned here will have broad implications for both civilian and combat casualty care as objective markers of wound failure are acutely needed.…”

Section: Discussionmentioning

confidence: 96%

Metalloproteinase Expression is Associated with Traumatic Wound Failure

Utz

Elster

Tadaki

et al. 2010

Journal of Surgical Research

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Section: Discussionmentioning

confidence: 96%

Metalloproteinase Expression is Associated with Traumatic Wound Failure

Utz

Elster

Tadaki

et al. 2010

Journal of Surgical Research

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“…The diagnostic and prognostic plaque or GCF markers for periodontitis include: 1) the presence of specific bacteria (including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans [previously Actinobacillus actinomycetemcomitans]); 49 2) bacterial products of metabolism (e.g., volatile sulfur compounds 50 or specific proteases; 51 3) biomarkers involved in the disease process but produced by the host (e.g., matrix metalloproteinases, 52 neutrophil elastase, and alkaline phosphatase); 53,54 4) biomarkers of tissue damage (e.g., hydroxyproline/collagen fragments); 53,55 and 5) other markers of the inflammatory process, such as prostaglandin E 2 and interleukin-1. 53 However, despite the complex nature of periodontal diseases, which involve a multifaceted immune and inflammatory reaction to a polymicrobial flora, and the interindividual variations in inflammatory responses, 56 such potential biomarkers are generally studied individually or, rarely, in small numbers.…”

Section: Diagnosis Of Periodontitis Based On Ir Spectra Of Gcfmentioning

confidence: 99%

An Update on Novel Non‐Invasive Approaches for Periodontal Diagnosis

Xiang

Sowa

Iacopino

et al. 2010

Journal of Periodontology

100

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READ THESE TERMS AND CONDITIONS CAREFULLY BEFORE USING THIS WEBSITE.Access and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en An Update on Novel Non-Invasive Approaches for Periodontal DiagnosisXiang, Xiaoming; Sowa, Michael G.; Iacopino , Anthony M.; Maev, Roman G.; Hewko, Mark D.; Man, Angela; Liu, Kan-Zhi Abstract For decades there has been an ongoing search for clinically acceptable methods for the accurate, non-invasive diagnosis and prognosis of periodontitis. There are several well-known inherent drawbacks with current clinical procedures. The purpose of this review is to summarize some of the newly emerging diagnostic approaches, namely, infrared spectroscopy, optical coherence tomography (OCT), and ultrasound. The history and attractive features of these new approaches are briefly illustrated, and the interesting and significant inventions related to dental applications are discussed. The particularly attractive aspects for the dental community are that some of these methods are totally non-invasive, do not impose any discomforts to the patients during the procedure, and require no tissue to be extracted. For instance, multiple inflammatory indices withdrawn from near infrared spectra have the potential to identify early signs of inflammation leading to tissue breakdown. Morphologically, some other non-invasive imaging modalities, such as OCT and ultrasound, could be employed to accurately measure probing depths and assess the status of periodontal attachment, the front-line of disease progression. Given that these methods reflect a completely different assessment of periodontal inflammation, if clinically validated, these methods could either replace traditional clinical examinations for the diagnosis of periodontitis or at least serve as attractive complementary diagnostic tools. However, the potential of these techniques should be interpreted more cautiously given the multifactorial character of periodontal disease. In addition to these novel tools in the field of periodontal inflammatory diseases, other alternative modalities like microbiologic and genetic approaches are only briefly mentioned in this review because they have been thoroughly discussed in other comprehensive reviews.Periodontitis is an endemic infectious disease of the tissues surrounding the teeth occurring in 50% of the population and may result in significant debilitation for about half of these persons. 1As the leading cause of edentulism, periodontitis may also have important implications for systemic health including an increased risk for cardiovascular disease and low birth weight/preterm delivery.2 According to a report by the World Health Organization, 3 severe periodontitis leading to tooth loss was found in 5% to 15% of most populations worldwide. Therefore, it is considered one of the most important global oral health burdens.Periodontitis is a multifactorial disease with microbial dental plaque as the in...

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“…En la actualidad ya existen algunos dispositivos de uso en clínica para determinar experimentalmente los niveles de estos biomarcadores, particularmente para la MMP-8. Al respecto se ha visto que las variaciones en los niveles de esta enzima pueden diferenciar sujetos sanos, de sujetos con gingivitis y periodontitis, como también evaluar la respuesta al tratamiento periodontal e identificar sujetos en riesgo de progresión (26)(27)(28) . De este modo, una herramienta no invasiva para detectar la presencia de inflamación periapical como el FCG sería de gran utilidad como complemento a la evaluación clínico-radiográfica de dientes con PAA, particularmente en aquellos casos en que la interpretación radiográfica sea incierta (29) .…”

Section: Discussionunclassified

Asociación entre niveles de TNF-α en fluido crevicular gingival de dientes con periodontitis apical asintomática

Flores

Vitali

Cabrera

et al. 2011

Rev. Clin. Periodoncia Implantol. Rehabil. Oral

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RESUMENIntroducción: Como consecuencia de necrosis pulpar séptica, el ingreso de productos bacterianos en el periápice induce la producción de citoquinas pro-inflamatorias como el Factor de Necrosis Tumoral (TNF)-, que entre otras funciones participa en la diferenciación y activación de los osteoclastos para inducir reabsorción ósea, fenómeno característico de la periodontitis apical asintomática (PAA). El fluido crevicular gingival (FCG) ofrece un gran potencial como fuente de factores asociados con la actividad osteoclástica. El objetivo del presente estudio fue determinar los niveles de TNFen FCG de dientes con PAA y controles sanos contralaterales. Métodos: Se incluyeron 14 pacientes en la Clínica de Endodoncia de la Facultad de Odontología de la Universidad de Chile con diagnóstico clínico de PAA y se obtuvieron muestras de FCG con tiras de papel a partir de los dientes afectados y sanos contralaterales por un periodo estandarizado de 30 segundos. Se determinaron las concentraciones de proteínas totales mediante el método del ácido bisciconitico y los niveles de TNF-, mediante ensayo ELISA. Los datos se analizaron con Test-t pareado utilizando el programa StataV11. Resultados: Se detectaron niveles de TNF-significativamente mayores en el FCG de dientes con PAA estandarizados, tanto por 30 segundos de toma de muestra como por mg de proteínas totales. Conclusiones: Este estudio provee evidencia preliminar de que los niveles de TNF-en el FCG reflejan la presencia de PAA y podría ser de utilidad como complemento al diagnóstico clínico y monitoreo del estado de salud o enfermedad de los tejidos perirradiculares. ABSTRACT Introduction: As a consequence of septic pulp necrosis, the entry of bacterial products into periapical tissues induces the release of pro-inflammatory cytokines, such as the tumor necrosis factor (TNF)-. This pleiotropic cytokine is involved in the differentiation and activation of osteoclasts to induce bone resorption, a hallmark of asymptomatic apical periodontitis (AAP). Gingival crevicular fluid (GCF) has a great potential as a source of factors associated with osteoclastic activity. The aim of this study was to determine the levels of TNF-in GCF of teeth with PAA and contralateral healthy controls. Methods: A total of 14 patients with clinical diagnosis of AAP were enrolled from the Clinic of Endodontics, Faculty of Dentistry, Universidad de Chile during 2009 and 30-second GCF samples were obtained with paper strips from AAP teeth and contralateral healthy controls. Total protein concentration and TNF-levels were determined through bisciconitic acid method and ELISA assay, respectively. Paired t test and StataV11 software were used for statistical analysis. Results: Levels of TNF-were significantly higher in GCF from teeth with AAP than controls when standardized by either 30s of sampling and total protein content. Conclusions: The present study provides preliminary data supporting that TNF-levels in GCF reflect periapical status. Screening of TNF-levels in GCF might represent a useful sid...

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Evaluation of Immunoassay‐Based MMP‐8 Detection in Gingival Crevicular Fluid on a Point‐of‐Care Platform

Cited by 23 publications

References 8 publications

Metalloproteinase Expression is Associated with Traumatic Wound Failure

Metalloproteinase Expression is Associated with Traumatic Wound Failure

An Update on Novel Non‐Invasive Approaches for Periodontal Diagnosis

Asociación entre niveles de TNF-α en fluido crevicular gingival de dientes con periodontitis apical asintomática

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