Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle

Scott, Katherine Anne; Rathogwa, N.M.; Capozzo, Alejandra Victoria; Maree, Francois Frederick

doi:10.1016/j.vaccine.2017.02.003

Cited by 10 publications

(12 citation statements)

References 46 publications

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“…Capozzo et al (1997) [38] reported that the ability of a FMD vaccine to raise a stronger IgG1 response correlates with the ability of vaccinated animals to be protected. Our data confirms evidence obtained by Scott et al (2017b) [35] supporting the role of the IgG1 isotype in recovery and protection of cattle from FMD.…”

Section: Discussionsupporting

confidence: 93%

“…The importance of a cell-mediated immune response against FMDV infection has been reported previously [35,39,50,51]. Consequently, the role of regulating the cell-mediated T-lymphocytes was measured by IFN-γ, which is mainly produced by activated cells in anamnestic responses.…”

Section: Discussionmentioning

confidence: 97%

“…Consequently, the role of regulating the cell-mediated T-lymphocytes was measured by IFN-γ, which is mainly produced by activated cells in anamnestic responses. IFN-γ levels measured from whole blood are extremely variable [ 52 ]; however, validation and implementation of the test was based on previous findings [ 39 ] and performed as described previously [ 35 ]. Significantly ( p < 0.05) higher levels of systemic IFN-γ responses were elicited 14 dpv and peaked at 28 dpv in animals vaccinated with the Montanide ISA 206B adjuvanted vaccine (8.9 ± 7.4 ng/mL), followed by SAT2-Quil-A Saponin (6.6 ± 5.6 ng/mL) vaccinated animals.…”

Section: Discussionmentioning

confidence: 99%

“…Total antibody titres in vaccinated cattle were detected with a SAT/ZIM/7/83-specific liquid-phase blocking enzyme-linked immunosorbent assay (ELISA) (LPBE) [ 15 , 35 ]. Antibodies were measured in serum collected on days 0, 2, 4, 7, 9, 11, 14, and 21 pv and thereafter every fortnight until 162 dpv.…”

Section: Methodsmentioning

confidence: 99%

“…The IgG1 and IgG2 isotype ELISAs were performed according to Capozzo et al (1997) [38], except that 100 ng of SDG-purified 146S SAT2/ZIM/7/83 was used per well [35]. A series of two-fold dilutions of test sera starting from 1:50 was prepared.…”

Section: Igg1 and Igg2 Isotyping Elisamentioning

confidence: 99%

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Efficacy of SAT2 Foot-and-Mouth Disease Vaccines Formulated with Montanide ISA 206B and Quil-A Saponin Adjuvants

et al. 2021

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The effective control of foot-and-mouth disease (FMD) relies strongly on the separation of susceptible and infected livestock or susceptible livestock and persistently infected wildlife, vaccination, and veterinary sanitary measures. Vaccines affording protection against multiple serotypes for longer than six months and that are less reliant on the cold chain during handling are urgently needed for the effective control of FMD in endemic regions. Although much effort has been devoted to improving the immune responses elicited through the use of modern adjuvants, their efficacy is dependent on the formulation recipe, target species and administration route. Here we compared and evaluated the efficacy of two adjuvant formulations in combination with a structurally stabilized SAT2 vaccine antigen, designed to have improved thermostability, antigen shelf-life and longevity of antibody response. Protection mediated by the Montanide ISA 206B-adjuvanted or Quil-A Saponin-adjuvanted SAT2 vaccines were comparable. The Montanide ISA 206B-adjuvanted vaccine elicited a higher SAT2 neutralizing antibody response and three times higher levels of systemic IFN-γ responses at 14- and 28-days post-vaccination (dpv) were observed compared to the Quil-A Saponin-adjuvanted vaccine group. Interestingly, serum antibodies from the immunized animals reacted similarly to the parental vaccine virus and viruses containing mutations in the VP2 protein that simulate antigenic drift in nature.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Igg1 and Igg2 Isotyping Elisamentioning

confidence: 99%

See 3 more Smart Citations

Efficacy of SAT2 Foot-and-Mouth Disease Vaccines Formulated with Montanide ISA 206B and Quil-A Saponin Adjuvants

et al. 2021

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Efficacy of a foot-and-mouth disease vaccine against a heterologous SAT1 virus challenge in goats

Lazarus

Peta

Blight

et al. 2020

Vaccine

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Systemic antibodies administered by passive immunization prevent generalization of the infection by foot-and-mouth disease virus in cattle after oronasal challenge

Barrionuevo

Giacomo²,

Bucafusco

et al. 2018

Virology

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The role of passively transferred sera in the protection against aerogenous foot-and-mouth disease (FMD) virus infection in cattle was evaluated using vaccine-induced immune serum preparations obtained at 7 and 26 days post-vaccination (dpv). We showed that circulating antibodies were sufficient to prevent disease generalization after oronasal infection in animals passively transferred with 26-dpv serum but not with the 7-dpv serum. Conversely, conventional FMD vaccination provided clinical protection at 7 dpv, promoting fast and robust antibody responses upon challenge and even though antibody titers were similar to those found in animals passively immunized with 7-dpv serum. These results demonstrate that presence of antigen-specific antibodies is critical to prevent the dissemination of the virus within the animal. Conventional FMD vaccination additionally promoted the deployment of rapid, high titer and isotype-switched antibody responses at systemic and mucosal levels after infection, thus conferring protection even in the presence of low pre-challenge antibody titers.

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Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle

Cited by 10 publications

References 46 publications

Efficacy of SAT2 Foot-and-Mouth Disease Vaccines Formulated with Montanide ISA 206B and Quil-A Saponin Adjuvants

Efficacy of SAT2 Foot-and-Mouth Disease Vaccines Formulated with Montanide ISA 206B and Quil-A Saponin Adjuvants

Efficacy of a foot-and-mouth disease vaccine against a heterologous SAT1 virus challenge in goats

Systemic antibodies administered by passive immunization prevent generalization of the infection by foot-and-mouth disease virus in cattle after oronasal challenge

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